miR-21-5p Alleviates OGD/R-induced Inflammation and Apoptosis of HT22 Cells and Promotes Proliferation by Inhibiting STAT3

Yanping LI; Xiaolin¹ DONG; Qingyun LI; Hongmei LI; Huan WEI; Yi ZENG

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Yanping LI, Xiaolin¹ DONG, Qingyun LI, Hongmei LI, Huan WEI, Yi ZENG. miR-21-5p Alleviates OGD/R-induced Inflammation and Apoptosis of HT22 Cells and Promotes Proliferation by Inhibiting STAT3[J]. Journal of Kunming Medical University.

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miR-21-5p Alleviates OGD/R-induced Inflammation and Apoptosis of HT22 Cells and Promotes Proliferation by Inhibiting STAT3

1.
Dept. of Neurology，Yan’an Hospital Affiliated to Kunming Medical University，Kunming Yunnan 650051
2.
Dept. of Neurosurgery，Chenggong Hospital of Yan’an Hospital Affiliated to Kunming Medical University，Kunming Yunnan 650500，China

Received Date: 2024-07-11
Available Online: 2024-11-23

Abstract

Abstract

Objective To investigate the effect of miR-21-5p on the injury of OGD/R-induced HT22 cells. Methods OGD / R induced HT22 cells to construct a cell model. The Cell Counting Kit-8（CCK-8） and flow cytometry were used to assess cell proliferation vitality and cell apoptosis. Western blot analysis was used to check STAT3 protein expression, and RT-qPCR was used to detect inflammatory factors expression.The Starbase database predicted the binding sites of miR-21-5p and STAT3. The dual luciferase assay verified the targeting relationship between miR-21-5p and STAT3. Results miR-21-5p was significantly decreased in OGD / R-induced neuronal cells, and p-STAT3 / STAT3 was significantly increased in OGD / R-induced neuronal cells. MiR-21-5p could target the expression of STAT3. Increased expression of miR-21-5p could significantly inhibit apoptosis and inflammation, and promote proliferation. Stattic could reverse the effect of miR-21-5p. Conclusion miR-21-5p alleviates OGD-induced inflammation and apoptosis in HT22 cells by inhibiting STAT3 and promotes proliferation.
- miR-21-5p,
- STAT3,
- Cerebral ischemia,
- Inflammation,
- Apoptosis

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References(20)

References

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