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RSS2011 Vol. 32, No. 02
2011, 32(02)
Abstract:
Objective To investigate the expression of NGF,BDNF,NT-3 and their receptors trkA,trkB,trkC in cultured Neural stem cells(NSCs).Methods NSCs were isolated from embryonic hippocampus of mice.They were then cultured in vitro and identified by immunohistochemistry using nestin antibody.The expression of mRNA and protein for NGF,BDNF,NT-3 was detected by RT-PCR and immunohistochemistry.Results Significant levels of mRNA were detected for all three factors and their receptor trkA,trkB,trkC by RT-PCR.The proteins...
Objective To investigate the expression of NGF,BDNF,NT-3 and their receptors trkA,trkB,trkC in cultured Neural stem cells(NSCs).Methods NSCs were isolated from embryonic hippocampus of mice.They were then cultured in vitro and identified by immunohistochemistry using nestin antibody.The expression of mRNA and protein for NGF,BDNF,NT-3 was detected by RT-PCR and immunohistochemistry.Results Significant levels of mRNA were detected for all three factors and their receptor trkA,trkB,trkC by RT-PCR.The proteins...
2011, 32(02)
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Objective To establish a animal model of bile duct strictures by electrocautery injury in Japanese rabbits,for studing the prevention and treatment of bile duct stricture.Methods 30 Japanese rabbits were randomly divided into control group(n=15)and experimental group(n=15),sham operation and electrocautery injury of bile duct were applied respectively.The general condition and survival of animals were observed after operation.The hepatic function of animals were examined before operation and after 30 days.T...
Objective To establish a animal model of bile duct strictures by electrocautery injury in Japanese rabbits,for studing the prevention and treatment of bile duct stricture.Methods 30 Japanese rabbits were randomly divided into control group(n=15)and experimental group(n=15),sham operation and electrocautery injury of bile duct were applied respectively.The general condition and survival of animals were observed after operation.The hepatic function of animals were examined before operation and after 30 days.T...
2011, 32(02)
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Objective To establish the primary culture and induced differentiation to fat cells of human omental adipose derived mesechymal stem cells(ADSCs).Methods The omental adipose tissues were obtained by opration and fibroblast-like cells was primarily cultured by collagenase digestion.The ADSCs were identificated by detecting the expression of CD44,CD34,CD90 and CD45 with flow cytometry,cell growth curve was measured by MTT method,the P3 cells were induced to fat cells with 3-isobutyl-1-methylxanthine,insulin,d...
Objective To establish the primary culture and induced differentiation to fat cells of human omental adipose derived mesechymal stem cells(ADSCs).Methods The omental adipose tissues were obtained by opration and fibroblast-like cells was primarily cultured by collagenase digestion.The ADSCs were identificated by detecting the expression of CD44,CD34,CD90 and CD45 with flow cytometry,cell growth curve was measured by MTT method,the P3 cells were induced to fat cells with 3-isobutyl-1-methylxanthine,insulin,d...
A Clinical Investigation of Compound Dilong Capsule in the Treatment of Type 2 Diabetics Nephropathy
2011, 32(02)
Abstract:
Objective To investigate the clinical value of compound Dilong capsule in the treatment of type 2 diabetics nephropathy.Methods 56 patients with type 2 diabetic nephropathy were randomly divided into compound Dilong group(n=30)and control group(n=26).Haemorheological parameters and total urine protein for 24 h were detected,respectively,before and after treatment.Results Compared with before treatment,the effective rate in compound Dilong capsule group was 85.6%,and 82.5% in the control group.There was no s...
Objective To investigate the clinical value of compound Dilong capsule in the treatment of type 2 diabetics nephropathy.Methods 56 patients with type 2 diabetic nephropathy were randomly divided into compound Dilong group(n=30)and control group(n=26).Haemorheological parameters and total urine protein for 24 h were detected,respectively,before and after treatment.Results Compared with before treatment,the effective rate in compound Dilong capsule group was 85.6%,and 82.5% in the control group.There was no s...
2011, 32(02)
Abstract:
Objective To study the results of the treatment of left hepatolithiasis by left external lobectomy.Method 79 cases of left hepatolithiasis which were treated by left external lobectomy in our department during the past 4 years were retrospectively analyzed.Results All 79 cases were performed lobectomy which included:15 cases of left external lobectomy,48 cases of left external lobectomy together with choledochotomy,16 cases of left external lobectomy,bile duct exploration and cholangioenterostomy.All patien...
Objective To study the results of the treatment of left hepatolithiasis by left external lobectomy.Method 79 cases of left hepatolithiasis which were treated by left external lobectomy in our department during the past 4 years were retrospectively analyzed.Results All 79 cases were performed lobectomy which included:15 cases of left external lobectomy,48 cases of left external lobectomy together with choledochotomy,16 cases of left external lobectomy,bile duct exploration and cholangioenterostomy.All patien...
2011, 32(02)
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Objective To explore the correlation between cancer pain and depression in elderly patients,and the effect of pain and depression on immune function.Methods 49 elderly cancer patients with pain and 60 aged cancer patients without pain controls(diagnosed by pathology)were tested with Self-Rating Depression scale(SDS),Simultaneous venous blood samples were collected from all of the 109 patients for measurement of immune function.Results There were no obvious statistical differences in the incidence rate of de...
Objective To explore the correlation between cancer pain and depression in elderly patients,and the effect of pain and depression on immune function.Methods 49 elderly cancer patients with pain and 60 aged cancer patients without pain controls(diagnosed by pathology)were tested with Self-Rating Depression scale(SDS),Simultaneous venous blood samples were collected from all of the 109 patients for measurement of immune function.Results There were no obvious statistical differences in the incidence rate of de...
2011, 32(02)
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Objective To explore the mental health status and personality of internet game addicts.Method IAT,SCL-90,and EPQ were used to test 79 internet game players from the game of WOW which is most popular in China.Results In SCL-90,the total score and all item's scores apart from psychoticism and others of addicts were higher than nonaddicts'(P<0.01).In EPQ,addict's scores in E demention were lower than nonadicts'(P<0.01),addict's scores in dementions of P,N and L were higher than nonadicts'(P<0.01).Conclusion Th...
Objective To explore the mental health status and personality of internet game addicts.Method IAT,SCL-90,and EPQ were used to test 79 internet game players from the game of WOW which is most popular in China.Results In SCL-90,the total score and all item's scores apart from psychoticism and others of addicts were higher than nonaddicts'(P<0.01).In EPQ,addict's scores in E demention were lower than nonadicts'(P<0.01),addict's scores in dementions of P,N and L were higher than nonadicts'(P<0.01).Conclusion Th...
2011, 32(02)
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Objective To explore the relationship between the expressions of CDK2 and p27kip1 proteinand biological behavior of non-small cell lung cancer in Xuanwei.Methods Immuneohistochemical method was used to detect the expressions of CDK2 and p27kip1 protein in lung cancer tissues and normal pulmonary tissuesof patients with non-small cell lung cancer in Xuanwei and non-Xuanwei regions.Results The expression of CDK2 protein was situated in endochylema in cancer tissues.The expression of CDK2 protein was the highe...
Objective To explore the relationship between the expressions of CDK2 and p27kip1 proteinand biological behavior of non-small cell lung cancer in Xuanwei.Methods Immuneohistochemical method was used to detect the expressions of CDK2 and p27kip1 protein in lung cancer tissues and normal pulmonary tissuesof patients with non-small cell lung cancer in Xuanwei and non-Xuanwei regions.Results The expression of CDK2 protein was situated in endochylema in cancer tissues.The expression of CDK2 protein was the highe...
2011, 32(02)
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Objective To investigate the effect of early swimming on the intelligence development of infants.Methods The objects in this study were infants aging from four hours to thirty days after birth.Every time they swimmed fifteen to twenty minutes and ten days as a course of treatment and at least two courses were required.Twelve intelligence indexex were observed for one to seven months.We evaluated the intelligence development according to the standards from the paidonosology in which the normal time expected ...
Objective To investigate the effect of early swimming on the intelligence development of infants.Methods The objects in this study were infants aging from four hours to thirty days after birth.Every time they swimmed fifteen to twenty minutes and ten days as a course of treatment and at least two courses were required.Twelve intelligence indexex were observed for one to seven months.We evaluated the intelligence development according to the standards from the paidonosology in which the normal time expected ...
2011, 32(02)
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Objective To study the significance of nutritional support in gastric cancer patients with nutritional risk during perioperative period secreened by NRS2002 scoring system.Methods The nutritional risk of gastric cancer patients was secreened by NRS2002 scoring system.The patients with nutritional risk was given enteral nutritional support before operation,and enteral and parenteral nutritional support after operation.The changes in body weight,serum albumin,CRP,the time of passage of gas by anus,the inciden...
Objective To study the significance of nutritional support in gastric cancer patients with nutritional risk during perioperative period secreened by NRS2002 scoring system.Methods The nutritional risk of gastric cancer patients was secreened by NRS2002 scoring system.The patients with nutritional risk was given enteral nutritional support before operation,and enteral and parenteral nutritional support after operation.The changes in body weight,serum albumin,CRP,the time of passage of gas by anus,the inciden...
2011, 32(02)
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Objective To compare the clinical characteristics of newborn respiratory distress syndrome between full-term and premature infants,and to further explore the similarities and differences between them.Methods Eighty-six cases of confirmed respiratory distress syndrome with different gestational age in ICU of the department of Newborn in our hospital from January,2007 to August,2010,were enrolled in this study.They were divided into two groups:the premature infant group(50 cases,<37 weeks)and the full-term gr...
Objective To compare the clinical characteristics of newborn respiratory distress syndrome between full-term and premature infants,and to further explore the similarities and differences between them.Methods Eighty-six cases of confirmed respiratory distress syndrome with different gestational age in ICU of the department of Newborn in our hospital from January,2007 to August,2010,were enrolled in this study.They were divided into two groups:the premature infant group(50 cases,<37 weeks)and the full-term gr...
2011, 32(02)
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Objective To investigate the expression and significance of plasminogen activator inhibitor type-1 in human glioma,and analyze its relationship with malignant biological behavior of human glioma,anddiscuss the role and significance of PAI-1 in human glioma.Methods PAI-1 protein expression was detected by immunohistochemical(ABC) method and ECL-Western blot in surgical samples of 41 patients with human glioma and 10 samples of normal brain tissue obtained from craniocerebral injury surgery.Results Along with...
Objective To investigate the expression and significance of plasminogen activator inhibitor type-1 in human glioma,and analyze its relationship with malignant biological behavior of human glioma,anddiscuss the role and significance of PAI-1 in human glioma.Methods PAI-1 protein expression was detected by immunohistochemical(ABC) method and ECL-Western blot in surgical samples of 41 patients with human glioma and 10 samples of normal brain tissue obtained from craniocerebral injury surgery.Results Along with...
2011, 32(02)
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Objective To investigate the inducement,incidence,treatment,outcome and prognosis of patients with chronic renal failure and pulmonary infection simultaneously,and provide envidences for the treatment of those patients.Methods We retrospectively analyzed the clinical data of 65 patients with chronic renal failure with pulmonary infection from January 2007 to December 2009 in our department.The diagnosis and treatment of patients with chronic renal failure and pulmonary infection was analyzed.Results During ...
Objective To investigate the inducement,incidence,treatment,outcome and prognosis of patients with chronic renal failure and pulmonary infection simultaneously,and provide envidences for the treatment of those patients.Methods We retrospectively analyzed the clinical data of 65 patients with chronic renal failure with pulmonary infection from January 2007 to December 2009 in our department.The diagnosis and treatment of patients with chronic renal failure and pulmonary infection was analyzed.Results During ...
2011, 32(02)
Abstract:
Objective To observe the therapeutic effects of combination of Mecarbinate(Telmisartan)and Hydrochlorothiazide on primary hypertension.Methods Sixty-five patients with mild or moderate primary hypertension were randomly divided into two groups.Patients in the first group(35 persons)were given 80mg of Mecarbinate and 25mg of Hydrochlorothiazide each day,and patients in the second group(30 persons)were given only 80mg Mecarbinate each day.Patient in the two groups were given the treatment for 7 weeks.Results(...
Objective To observe the therapeutic effects of combination of Mecarbinate(Telmisartan)and Hydrochlorothiazide on primary hypertension.Methods Sixty-five patients with mild or moderate primary hypertension were randomly divided into two groups.Patients in the first group(35 persons)were given 80mg of Mecarbinate and 25mg of Hydrochlorothiazide each day,and patients in the second group(30 persons)were given only 80mg Mecarbinate each day.Patient in the two groups were given the treatment for 7 weeks.Results(...
2011, 32(02)
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Objective To compare the positive rate of asthmatic patients in provocative test between 24 h and 72 h after stop using bronchodilators.Method 200 suspected asthmatic patients were given provocative test by inhalating methacholine with Jaeger MASTERS OCOPE APSPRO spirometry 24 h and 72 h after stop using bronchodilators.Result The positive rate of asthmatic patients in provocative test 72h after stop using bronchodilators was higher than 24 h after stop using bronchodilators,the difference had statistical s...
Objective To compare the positive rate of asthmatic patients in provocative test between 24 h and 72 h after stop using bronchodilators.Method 200 suspected asthmatic patients were given provocative test by inhalating methacholine with Jaeger MASTERS OCOPE APSPRO spirometry 24 h and 72 h after stop using bronchodilators.Result The positive rate of asthmatic patients in provocative test 72h after stop using bronchodilators was higher than 24 h after stop using bronchodilators,the difference had statistical s...
2011, 32(02)
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Objective To study the chief inducement and prognosis of acute kidney injury on the basis of chronic kindney disease(AKI on CKD,A/C).Methods From July 2004 to July 2010,the clinical data(including the inducement and prognosis)of 56 patients with AKI on CKD was retrospectively analyzed.Results The major causes of A/C were:severe infection(37.5%),severe hypertension(23.2%),the progression of primary renal disease(19.6%).The prognosis of A/C:the recovery of renal function(82.1%),no change(10.71%),chronic failu...
Objective To study the chief inducement and prognosis of acute kidney injury on the basis of chronic kindney disease(AKI on CKD,A/C).Methods From July 2004 to July 2010,the clinical data(including the inducement and prognosis)of 56 patients with AKI on CKD was retrospectively analyzed.Results The major causes of A/C were:severe infection(37.5%),severe hypertension(23.2%),the progression of primary renal disease(19.6%).The prognosis of A/C:the recovery of renal function(82.1%),no change(10.71%),chronic failu...
2011, 32(02)
Abstract:
Objective To study the association between XAGE-1 gene expression and the clinical characteristics of gastric cancer.Methods Tumor tissue and adjacent tissue specimens from 85 patients with gastric cancer were collected,and the total of RNA was extracted to detect the expression of the four XAGE-1 transcript variants by nest PCR.The correlation between XAGE-1 gene expression and several clinical and pathologic variables were analyzed.Results 49.41%(42/85) of gastric cancer samples were positive for XAGE-1.T...
Objective To study the association between XAGE-1 gene expression and the clinical characteristics of gastric cancer.Methods Tumor tissue and adjacent tissue specimens from 85 patients with gastric cancer were collected,and the total of RNA was extracted to detect the expression of the four XAGE-1 transcript variants by nest PCR.The correlation between XAGE-1 gene expression and several clinical and pathologic variables were analyzed.Results 49.41%(42/85) of gastric cancer samples were positive for XAGE-1.T...
2011, 32(02)
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Objective To study the clinical value of loop electrosurgical excision procedure(LEEP)in the treatment of cervical intraepithelial neoplasia(CIN).Methods LEEP was performed in 206 cases of CIN after multiple punch biopsy under colposcopy treatment in our hospital between August 2006 and August 2009.The symptoms,operative time,volume of bleeding of patients were observed and compared with preoperative cervical biopsy and postoperation pathological examination findings,and patients were followed up to observe...
Objective To study the clinical value of loop electrosurgical excision procedure(LEEP)in the treatment of cervical intraepithelial neoplasia(CIN).Methods LEEP was performed in 206 cases of CIN after multiple punch biopsy under colposcopy treatment in our hospital between August 2006 and August 2009.The symptoms,operative time,volume of bleeding of patients were observed and compared with preoperative cervical biopsy and postoperation pathological examination findings,and patients were followed up to observe...
2011, 32(02)
Abstract:
Objective To evaluate the therapeutic effect of flupentixol and melitraeen combined with non-drug treatment on patients with chronic tension-type headache.Methods 64 patients with CTH were divided into two groups in this study:the treatment group(32 cases,non-drug treatment)and the control group(32 cases,drug treatment).The therapeutic effects were assessed 4 and 8 weeks after treatment.Result The effective rate of the treatment group was 100% and significantly higher than the control group at 4w(P<0.01)and...
Objective To evaluate the therapeutic effect of flupentixol and melitraeen combined with non-drug treatment on patients with chronic tension-type headache.Methods 64 patients with CTH were divided into two groups in this study:the treatment group(32 cases,non-drug treatment)and the control group(32 cases,drug treatment).The therapeutic effects were assessed 4 and 8 weeks after treatment.Result The effective rate of the treatment group was 100% and significantly higher than the control group at 4w(P<0.01)and...
2011, 32(02)
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Objective To evaluate the safety and superiority of the laparoscopic hysterectomy.Methods 138 patients who need to take the hysterectomy were divided randomly into laparoscopic group(A group n=69) and laparotomy group(B group n=69).The operation time,blood loss during operation,postoperative recovery of bowel function,the length of stay in hospital and the complications of patients were compared between the two groups.Results The average operation time of patients in the laparoscopic group was a little long...
Objective To evaluate the safety and superiority of the laparoscopic hysterectomy.Methods 138 patients who need to take the hysterectomy were divided randomly into laparoscopic group(A group n=69) and laparotomy group(B group n=69).The operation time,blood loss during operation,postoperative recovery of bowel function,the length of stay in hospital and the complications of patients were compared between the two groups.Results The average operation time of patients in the laparoscopic group was a little long...
2011, 32(02)
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Objective To observe the effects of target controlled infusion(TCI)of propofol on the blood pressure variability of patients with high altitude hypertension during general anesthesia induction.Methods Forty patients with high altitude hypertension were randomly divided into two groups:TCI group(n=20)and normal group(n=20).Patients in group TCI were given Propofol by using Diprifusor/TCI system according target concentration,and patients in normal group was injected manually with Propofol.Invasive blood pres...
Objective To observe the effects of target controlled infusion(TCI)of propofol on the blood pressure variability of patients with high altitude hypertension during general anesthesia induction.Methods Forty patients with high altitude hypertension were randomly divided into two groups:TCI group(n=20)and normal group(n=20).Patients in group TCI were given Propofol by using Diprifusor/TCI system according target concentration,and patients in normal group was injected manually with Propofol.Invasive blood pres...
2011, 32(02)
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Objective To explore the feasibility and clinical value of trepan stamp graft in lumbar interbody fusion.Methods From January 2000 to April 2008,sixty patients with lumbar disease who need lumbar fusion were randomly divided into 2 groups:observation group and control group.Patients in the observation group were given trepan stamp graft,and patients in the control group were given intervertebral fusion cage.The fusion time,fusion rate,intervertebral height changes,and complications were compared between the...
Objective To explore the feasibility and clinical value of trepan stamp graft in lumbar interbody fusion.Methods From January 2000 to April 2008,sixty patients with lumbar disease who need lumbar fusion were randomly divided into 2 groups:observation group and control group.Patients in the observation group were given trepan stamp graft,and patients in the control group were given intervertebral fusion cage.The fusion time,fusion rate,intervertebral height changes,and complications were compared between the...
2011, 32(02)
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Objective To explore the therapeutic effects of combination of ozone and collagenase on lubar intervertebral disc protrusion.Methods Ninety-seven patients with lubar intervertebral disc protrusion were enrolled in this study.21G atraumatic puncture needle was punctured into astern 1/3 site of intervertebral disc guided by X-ray localization,then ozone at 60 mg/L was injected slowly by 15 mL/disk.CT scaning was used to learn the distribution of ozone in intervertebral disc in 5 min after injection of ozone.2...
Objective To explore the therapeutic effects of combination of ozone and collagenase on lubar intervertebral disc protrusion.Methods Ninety-seven patients with lubar intervertebral disc protrusion were enrolled in this study.21G atraumatic puncture needle was punctured into astern 1/3 site of intervertebral disc guided by X-ray localization,then ozone at 60 mg/L was injected slowly by 15 mL/disk.CT scaning was used to learn the distribution of ozone in intervertebral disc in 5 min after injection of ozone.2...
2011, 32(02)
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Objective To analyze the clinical and pathological characteristics of autoimmune hepatitis.Method The clinical data of 92 patients with autoimmune hepatitis(16 patients with AIH,55 patients with PBC,21 patients with PBC-AIH overlap syndrome)were retrospectively analyzed in our department from January 2004 to May 2009.Results The male to female ratio of 92 patients was 1:4,and the average age was 49.52 years.Laboratory investigations revealed elevated serum ALT,AST,and IgG and positive antinuclear antibodies...
Objective To analyze the clinical and pathological characteristics of autoimmune hepatitis.Method The clinical data of 92 patients with autoimmune hepatitis(16 patients with AIH,55 patients with PBC,21 patients with PBC-AIH overlap syndrome)were retrospectively analyzed in our department from January 2004 to May 2009.Results The male to female ratio of 92 patients was 1:4,and the average age was 49.52 years.Laboratory investigations revealed elevated serum ALT,AST,and IgG and positive antinuclear antibodies...
2011, 32(02)
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Objective To investigate the levels of blood glucose in hospitalized non-diabetic patients with essential hypertension living in Kunming of Yunnan province and provide references for the reasonable treatment of the patients with essential hypertension.Methods The fasting plasma glucose(FPG),glycosylated serum protein(SFA),glycosylated hemoglobin(HbA1c)were detected in 464 patients with essential hypertension without other diseases.The levels of blood glucose in patients with essential hypertension alone(gro...
Objective To investigate the levels of blood glucose in hospitalized non-diabetic patients with essential hypertension living in Kunming of Yunnan province and provide references for the reasonable treatment of the patients with essential hypertension.Methods The fasting plasma glucose(FPG),glycosylated serum protein(SFA),glycosylated hemoglobin(HbA1c)were detected in 464 patients with essential hypertension without other diseases.The levels of blood glucose in patients with essential hypertension alone(gro...
2011, 32(02)
Abstract:
Objective To investigate the therapeutic effect of large dose of mucosolvan on respiratory distress syndrome in premature children.Methods Seventy-two premature children with NRDS were randomly divided into treatment group(37 cases)and control group(35 cases),all cases were given same combined therapy and variant flow rate continuous positive airway by nasal mask.The cases in treatment group were given 30 mg/(kg.d)of mucosolvan for four times.The clinical symptoms and blood gas analysis(PaO2,PaCO2,PaO2/FiO2...
Objective To investigate the therapeutic effect of large dose of mucosolvan on respiratory distress syndrome in premature children.Methods Seventy-two premature children with NRDS were randomly divided into treatment group(37 cases)and control group(35 cases),all cases were given same combined therapy and variant flow rate continuous positive airway by nasal mask.The cases in treatment group were given 30 mg/(kg.d)of mucosolvan for four times.The clinical symptoms and blood gas analysis(PaO2,PaCO2,PaO2/FiO2...
2011, 32(02)
Abstract:
Objective To explore whether the reform of examination system and the experimental teaching methods in pharmacological course can improve the comprehensive ability of students.Methods The method of problem based learning(PBL)was introduced based on the conventional teaching way.The examination system was practiced and the teaching effect was investigated and analyzed after the students had completed all the experimental courses.Results PBL teaching mode significantly improved students' autonomous learning a...
Objective To explore whether the reform of examination system and the experimental teaching methods in pharmacological course can improve the comprehensive ability of students.Methods The method of problem based learning(PBL)was introduced based on the conventional teaching way.The examination system was practiced and the teaching effect was investigated and analyzed after the students had completed all the experimental courses.Results PBL teaching mode significantly improved students' autonomous learning a...
2011, 32(02)
Abstract:
［Abstract］Objective To observe the effect of survivin gene expression inhibition by small RNA interference （siRNA）on the proliferation and apoptosis of the breast cancer SKBr-3 cells．Methods We designed and synthetized siRNA targeted survivin gene，and used liposome embedding method to transfect the siRNA into the breast cancer SKBr -3 cells．Then we used MTT method to detect cells proliferation rate，Western blot and RT-PCR to observe survivin mRNA and protein expression；Flow cytometry（FCM） to test cell apoptosis rate． Results MTT result showed the proliferation of cells in survivin-siRNA group was inhibited obviously（P＜0.01），the highest cell inhibition rate reached（43.1±0.3）%．RT-PCR result showed the survivin mRNA expression in survivin -siRNA groups was downregulated（P＜0.01），the relative expression of mRNA was 0.203±0.018 and 0.229±0.019，the inhibition ratio was 55.2% and 49.4%，respectively. Western blot result showed the survivin protein expression in survivin -siRNA groups was downregulated（P＜0.01），the relative expression was 0.702±0.007 and 0.684±0.016， the inhibition ratio was 34.8% and 36.4%， respectively. Flow cytometry result showed that cell apoptosis rate was increased obviously in survivin-siRNA groups（P＜0.01），which was（14.2±1.4）% and（16.8±0.6）%．Conclusions Survivin-siRNA can effectively inhibit survivin gene expression，thus inhibit SKBr-3 cell proliferation and induce apoptosis．Survivin gene may be a new target for breast cancer therapy，which provides theory basis for breast cancer gene therapy.
［Abstract］Objective To observe the effect of survivin gene expression inhibition by small RNA interference （siRNA）on the proliferation and apoptosis of the breast cancer SKBr-3 cells．Methods We designed and synthetized siRNA targeted survivin gene，and used liposome embedding method to transfect the siRNA into the breast cancer SKBr -3 cells．Then we used MTT method to detect cells proliferation rate，Western blot and RT-PCR to observe survivin mRNA and protein expression；Flow cytometry（FCM） to test cell apoptosis rate． Results MTT result showed the proliferation of cells in survivin-siRNA group was inhibited obviously（P＜0.01），the highest cell inhibition rate reached（43.1±0.3）%．RT-PCR result showed the survivin mRNA expression in survivin -siRNA groups was downregulated（P＜0.01），the relative expression of mRNA was 0.203±0.018 and 0.229±0.019，the inhibition ratio was 55.2% and 49.4%，respectively. Western blot result showed the survivin protein expression in survivin -siRNA groups was downregulated（P＜0.01），the relative expression was 0.702±0.007 and 0.684±0.016， the inhibition ratio was 34.8% and 36.4%， respectively. Flow cytometry result showed that cell apoptosis rate was increased obviously in survivin-siRNA groups（P＜0.01），which was（14.2±1.4）% and（16.8±0.6）%．Conclusions Survivin-siRNA can effectively inhibit survivin gene expression，thus inhibit SKBr-3 cell proliferation and induce apoptosis．Survivin gene may be a new target for breast cancer therapy，which provides theory basis for breast cancer gene therapy.
