miR-21-5p Targetes STAT3 Reduce the OGD/R-induced Neuronal Injury

Yanping LI; Qingyun LI; Rong HUO; Xiaolin DONG; Hongmei LI; Huan WEI; Yi ZENG

doi:10.12259/j.issn.2095-610X.S20250502

Volume 46 Issue 5

May 2025

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Article Navigation > Journal of Kunming Medical University > 2025 > 46(5): 12-20

Yanping LI, Qingyun LI, Rong HUO, Xiaolin DONG, Hongmei LI, Huan WEI, Yi ZENG. miR-21-5p Targetes STAT3 Reduce the OGD/R-induced Neuronal Injury[J]. Journal of Kunming Medical University, 2025, 46(5): 12-20. doi: 10.12259/j.issn.2095-610X.S20250502

Citation:

Yanping LI, Qingyun LI, Rong HUO, Xiaolin DONG, Hongmei LI, Huan WEI, Yi ZENG. miR-21-5p Targetes STAT3 Reduce the OGD/R-induced Neuronal Injury[J]. Journal of Kunming Medical University, 2025, 46(5): 12-20. doi: 10.12259/j.issn.2095-610X.S20250502

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miR-21-5p Targetes STAT3 Reduce the OGD/R-induced Neuronal Injury

doi: 10.12259/j.issn.2095-610X.S20250502

1.
Department of Neurology，Yan’an Hospital Affiliated to Kunming Medical University，Kunming Yunnan 650051
2.
Department of Neurosurgery，Chenggong Hospital of Yan’an Hospital Affiliated to Kunming Medical University，Kunming Yunnan 650500，China

Received Date: 2024-07-11
Publish Date: 2025-05-30

Abstract

Abstract

Objective To investigate the potential mechanism of miR-21-5p in alleviating cerebral ischemia-reperfusion injury by targeting STAT3. Methods The HT22 cells were induced by OGD/R to construct a cell model of cerebral ischemia reperfusion injury. The expression of miR-21-5p was detected by RT-qPCR. The CCK-8 assay, TUNEL staining and flow cytometry were respectively used to detect the cell viability and apoptosis. ELISA assay was used to determine the contents of inflammatory factors IL-6, IL-10 and TNF-α in the cell supernatant. Western blot was used to detect the expression levels of p-STAT3/STAT3, Cleaved-Caspase-3, Bax and Bcl-2 proteins. The TargetScan database was used to predict the binding sites of miR-21-5p and STAT3. The dual-luciferase reporter gene assay was used to verify the targeting relationship between miR-21-5p and STAT3. Results The relative expression level of miR-21-5p was down-regulated in HT22 cells which induced by OGD/R （P < 0.001）. The cell viability （P < 0.0001） was decreased and the apoptosis rate （P < 0.001） was increased in OGD/R induced-HT22 cells. The contents of pro-inflammatory factors IL-6 （P < 0.001） and TNF-α （P < 0.001） was increased, while the content of anti-inflammatory factor IL-10 （P < 0.001） decreased. After transfection with miR-21-5p mimic, cell viability was enhanced, apoptosis rate was reduced and neuroinflammation was inhibited. MiR-21-5p could target and bind to STAT3. After miR-21-5p inhibitor transfection, cell viability decreased, apoptosis was promoted, and neuroinflammation occurred; STAT3 inhibitor Stattic could reverse the effect of miR-21-5p inhibitor. Conclusion MiR-21-5p could specifically bind to STAT3 and reduce the neuroinflammation and apoptosis of OGD/R induced-HT22 cells.
- Cerebral ischemia reperfusion injury,
- miR-21-5p,
- STAT3,
- Oxygen-glucose deprivation/reoxygenation,
- Apoptosis,
- Neuroinflammation

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