Effect of mTOR Signaling Pathway Inhibition on Bleomycin-induced Pulmonary Fibrosis

Egao YIN; Wen LEI; Mei YANG; Yongjun LIU; Zhaoxing DONG

doi:10.12259/j.issn.2095-610X.S20250608

Volume 46 Issue 6

Jun. 2025

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Article Navigation > Journal of Kunming Medical University > 2025 > 46(6): 64-70

Egao YIN, Wen LEI, Mei YANG, Yongjun LIU, Zhaoxing DONG. Effect of mTOR Signaling Pathway Inhibition on Bleomycin-induced Pulmonary Fibrosis[J]. Journal of Kunming Medical University, 2025, 46(6): 64-70. doi: 10.12259/j.issn.2095-610X.S20250608

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Egao YIN, Wen LEI, Mei YANG, Yongjun LIU, Zhaoxing DONG. Effect of mTOR Signaling Pathway Inhibition on Bleomycin-induced Pulmonary Fibrosis[J]. Journal of Kunming Medical University, 2025, 46(6): 64-70. doi: 10.12259/j.issn.2095-610X.S20250608

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Effect of mTOR Signaling Pathway Inhibition on Bleomycin-induced Pulmonary Fibrosis

doi: 10.12259/j.issn.2095-610X.S20250608

Egao YIN^{1, 2},
Wen LEI²,
Mei YANG³,
Yongjun LIU⁴,
Zhaoxing DONG^{5
,
,}

1.
Respiratory Medicine，Aba Tibetan and Qiang Autonomous Prefecture People’s Hospital，Aba Sichuan 623200
2.
Respiratory and Critical Medicine Department，The Second Affiliated Hospital of Kunming Medical University，Kunming Yunnan 650101
3.
Respiratory and Critical Medicine Department，Affiliated Hospital of Yunnan University，Kunming Yunnan 650101
4.
Yunnan Normal University Hospital，Kunming Yunnan 650101
5.
Respiratory and Critical Care Medicine Department of the Second Hospital of Ningbo，Ningbo Zhejiang 315000，China

Received Date: 2024-05-25
Publish Date: 2025-06-25

Abstract

Abstract

Objective To investigate the effect of mTOR signaling pathway on bleomycin-induced pulmonary fibrosis. Methods 30 healthy male C57BL/6 mice aged 6～8 weeks were fed for 1 week and divided into control group （NC group, n = 5）, bleomycin group （BLM group, n = 5）, and rapamycin + bleomycin group （Rapa+BLM group, n = 5）. Mice were euthanized by cervical dislocation at 7 and 28 days, and lung tissues were collected. HE staining was used to observe inflammatory infiltration in lung tissue, and Masson's staining was used to assess the severity of lung fibrosis. Western blot and qPCR were used to detect the expression levels of collagen I, collagen III and α-SMA to evaluate the degree of lung fibrosis. Western blot was used to detect the expression of mTOR, P70S6K and their phosphorylation levels in each group. Results Compared with the NC group, the BLM group showed thickened alveolar septa, obvious inflammatory changes, and collagen deposition.The protein expression of Collagen I, Collagen III, and α-SMA were significantly increased （P < 0.01）, with increased mRNA expression of Collagen I, Collagen III, and α-SMA （P < 0.05）, and elevated p-mTOR and p-p70S6K expression （P < 0.05）. Compared with the BLM group, the Rapa+BLM group showed improved lung tissue structure, reduced inflammation and collagen deposition, a downward trend in Collagen I, Collagen III, and α-SMA protein expression （P > 0.05）, a downward trend in Collagen I mRNA （P > 0.05）, and decreased Collagen III and α-SMA mRNA expression （P < 0.05）. Conclusion Abnormal mTOR activation was observed in bleomycin-induced pulmonary fibrosis; inhibiting mTOR signaling pathway activation can effectively alleviate the formation of pulmonary fibrosis.
- Pulmonary fibrosis,
- Bleomycin,
- Rapamycin

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References(21)

References

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