Cultivation,Differentiation and Identification of Human Omental Adipose Derived Mesenchymal Stem Cells in Vitro

Objective To establish the primary culture and induced differentiation to fat cells of human omental adipose derived mesechymal stem cells(ADSCs).Methods The omental adipose tissues were obtained by opration and fibroblast-like cells was primarily cultured by collagenase digestion.The ADSCs were identificated by detecting the expression of CD44,CD34,CD90 and CD45 with flow cytometry,cell growth curve was measured by MTT method,the P3 cells were induced to fat cells with 3-isobutyl-1-methylxanthine,insulin,d...