Effect of Survivin Gene Expression Inhibition by RNAi on Breast Cancer SKBr-3 Cells

［Abstract］Objective　To observe the effect of survivin gene expression inhibition by small RNA interference （siRNA）on the proliferation and apoptosis of the breast cancer SKBr-3 cells．Methods　We designed and synthetized siRNA targeted survivin gene，and used liposome embedding method to transfect the siRNA into the breast cancer SKBr -3 cells．Then we used MTT method to detect cells proliferation rate，Western blot and RT-PCR to observe survivin mRNA and protein expression；Flow cytometry（FCM） to test cell apoptosis rate． Results　MTT result showed the proliferation of cells in survivin-siRNA group was inhibited obviously（P＜0.01），the highest cell inhibition rate reached（43.1±0.3）%．RT-PCR result showed the survivin mRNA expression in survivin -siRNA groups was downregulated（P＜0.01），the relative expression of mRNA was 0.203±0.018 and 0.229±0.019，the inhibition ratio was 55.2% and 49.4%，respectively. Western blot result showed the survivin protein expression in survivin -siRNA groups was downregulated（P＜0.01），the relative expression was 0.702±0.007 and 0.684±0.016， the inhibition ratio was 34.8% and 36.4%， respectively. Flow cytometry result showed that cell apoptosis rate was increased obviously in survivin-siRNA groups（P＜0.01），which was（14.2±1.4）% and（16.8±0.6）%．Conclusions　Survivin-siRNA can effectively inhibit survivin gene expression，thus inhibit SKBr-3 cell proliferation and induce apoptosis．Survivin gene may be a new target for breast cancer therapy，which provides theory basis for breast cancer gene therapy.