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RSS2025 Vol. 46, No. 1
2025, 46(1): 1-8.
doi: 10.12259/j.issn.2095-610X.S20250101
Abstract:
Thrombotic diseases refer to a group of conditions characterized by abnormal blood coagulation within blood vessels, leading to the formation of blood clots and a series of clinical symptoms. This includes diseases such as deep vein thrombosis in the lower extremities, stroke, atherosclerosis, and diabetes. Thrombosis is a complex, progressive process that can be broadly summarized as the exposure of vascular endothelial damage, activation of the intrinsic coagulation system, platelet adhesion and aggregation, fibrin network formation, blood cell stasis. Macrophages play an important role in this process. They are involved in local inflammatory responses, regulating both the formation and resolution of thrombi. Macrophage polarization is a hot research topic in recent years, which mainly refers to the morphological and functional changes of macrophages in response different environmental stimuli. Macrophage polarization can be classified into classical （M1） and alternative （M2） types, as well as several specialized polarization states. The transition of macrophage polarization states plays an important role in immune responses, pathogen infections, tumor immunity, and autoimmune processes. This review discusses the regulatory relationship of macrophage polarization in thrombotic diseases, providing new directions for the treatment of these conditions.
Thrombotic diseases refer to a group of conditions characterized by abnormal blood coagulation within blood vessels, leading to the formation of blood clots and a series of clinical symptoms. This includes diseases such as deep vein thrombosis in the lower extremities, stroke, atherosclerosis, and diabetes. Thrombosis is a complex, progressive process that can be broadly summarized as the exposure of vascular endothelial damage, activation of the intrinsic coagulation system, platelet adhesion and aggregation, fibrin network formation, blood cell stasis. Macrophages play an important role in this process. They are involved in local inflammatory responses, regulating both the formation and resolution of thrombi. Macrophage polarization is a hot research topic in recent years, which mainly refers to the morphological and functional changes of macrophages in response different environmental stimuli. Macrophage polarization can be classified into classical （M1） and alternative （M2） types, as well as several specialized polarization states. The transition of macrophage polarization states plays an important role in immune responses, pathogen infections, tumor immunity, and autoimmune processes. This review discusses the regulatory relationship of macrophage polarization in thrombotic diseases, providing new directions for the treatment of these conditions.
2025, 46(1): 9-15.
doi: 10.12259/j.issn.2095-610X.S20250102
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Objective To understand the refractive progression of myopic school-aged children in ethnic minority areas of Yunnan and explore related factors. Methods Based on an existing cohort, a first follow-up survey was conducted from October 2020 to June 2021 involving 1, 774 students selected from three ethnic minority areas: Dali City （Bai ethnic group）, Lijiang Ancient Town （Naxi ethnic group）, and Menghai County in Xishuangbanna （Dai and Hani ethnic groups）.Among them, 816 myopic school-aged children at baseline were selected as research subjects to analyze changes in refractive error （△SE） and the degree of refractive progression （classified as rapid △SE and slow △SE） over one year. Binary logistic regression was used to analyze the influencing factors of refractive progression. Results After one year of follow-up, the change in refractive error for myopic school-aged children was -0.63 （-1.00, -0.25） D, with 518 children experiencing rapid △SE and 298 children experiencing slow △SE. Comparisons by educational stage indicated that Dai ethnic group children in primary school were more likely to experience rapid △SE than those in junior high school （P < 0.05）. Logistic regression results showed that school-age children of Naxi ethnic group （OR = 1.879, 95%CI: 1.132～2.999） , and those who used their eyes in the classroom during breaks （OR = 1.541, 95%CI: 1.088～2.181） were at higher risk of rapid refractive progression （P < 0.05）. Children of school age who engage in outdoor activities for at least 3 hours during the day （OR = 0.539, 95%CI: 0.340～0.853） and those who frequently consume animal liver （OR = 0.596, 95%CI: 0.399～0.892） have a lower risk of rapid myopic progression （P < 0.05）. Conclusion Myopic school-aged children in ethnic minority areas of Yunnan exhibit rapid refractive progression, with significant ethnic differences. The refractive progression in these children is closely related to lifestyle habits, highlighting the need for targeted myopia prevention and control measures and research for children and adolescents in ethnic minority areas of Yunnan.
2025, 46(1): 16-22.
doi: 10.12259/j.issn.2095-610X.S20250103
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Objective To explore and analyze adverse drug events （ADE） signals of azithromycin, identify common adverse events and suspected adverse reactions that occur easily in the real clinical application of azithromycin. Methods The adverse drug event reports related to azithromycin recorded in the US FDA Adverse Event Reporting System database from October 1, 2003, to October 1, 2023, were mined using the OpenVigil 2.1 drug surveillance platform. Signal detection and analysis were carried out using the reporting odds ratio （ROR） and Bayesian confidence propagation neural network method （BCPNN）. Results A total of 5498 ADE signals were identified, with a total of 112485 reports. Eventually, 31, 041 azithromycin-associated adverse event reports were extracted, involving 898 positive signals, 27 system organ classes, mainly concentrated in respiratory system diseases, infections, gastrointestinal system diseases, and others. Additionally, some adverse reactions possibly caused by azithromycin were discovered, such as asthma, wheezing, and potential reactions like incomplete abortion. Conclusion When using azithromycin, besides paying attention to the adverse reactions listed in the drug instructions, we should also pay attention to some adverse reactions that have not been timely recorded or updated, in order to prevent the medication safety risks caused by outdated instructions, provide reasonable medication advice, and ensure the medication safety of patients.
2025, 46(1): 23-35.
doi: 10.12259/j.issn.2095-610X.S20250104
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Objective To utilize single-cell and peripheral blood transcriptomic data from 3D brain organoids, combined with machine learning, to analyze the role of mitochondrial autophagy genes in schizophrenia （SCZ）. Methods By integrating two machine learning algorithms, we identified differentially expressed mitochondrial autophagy-related genes between schizophrenia patients and healthy controls using peripheral blood RNA sequencing data. The relationship between mitophagy gene, immune cells and inflammatory factors was further explored. Comprehensive single-cell analysis was used to explore the signaling pathways and specific transcription factors based on mitophagy genes. Results Using machine learning, seven key mitophagy genes expressed in schizophrenia patients were identified. Based on Mitoscore analysis, at the single-cell level, neurons with high mitochondrial autophagy activity （Mitohigh_Neuron） formed new interactions with endothelial cells via the SPP1 signaling pathway. Conclusion This study identified two subtypes of mitophagy and seven key mitophagy genes in schizophrenia, providing new insights into the pathogenesis of the disease.
Galangin Inhibits the Migration and Invasion of Cervical Cancer Hela Cells Through Hippo/YAP Pathway
2025, 46(1): 36-42.
doi: 10.12259/j.issn.2095-610X.S20250105
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Objective To investigate the effects of galangin on the migration and invasion abilities of cervical cancer Hela cells and its potential mechanisms. Methods Hela cells were treated with different concentrations of galangin （0, 5, 10, 20, 40, 60, 80, 100 µmol/L） for 48 hours, and CCK-8 assay was used to assess the impact of galangin on cell viability and to determine the half-maximal lethal concentration （IC50） of galangin. Hela cells were divided into a control group （0 μmol/L） and a galangin group （40 μmol/L treatment）. Scratch wound healing assays and Transwell chamber assays were conducted to evaluate the migration and invasion abilities of the cells in each group. Western Blot was used to detect the protein expression of E-cadherin and N-cadherin. DIA quantitative proteomics technology was used to detect and screen the differentially expressed proteins between the two groups. Biological function enrichment analysis of the differential genes was performed using the KEGG Pathway and Gene Set Enrichment Analysis （GSEA） methods. Western Blot was used to verify the expression levels of Hippo/YAP signaling pathway-related proteins YAP and p-YAP. Results Compared to the control group, galangin （40 μmol/L） significantly inhibited the viability of Hela cells in a concentration-dependent manner （P < 0.001）. Compared with the control group, the scratch healing ability and invasion ability of cervical cancer Hela cells treated with galangin（40 μmol/L） were significantly reduced （P < 0.001）. The expression of E-cadherin protein was increased （P < 0.05） and the expression of N-cadherin protein was decreased （P < 0.001） in the galangin group （40 μmol/L） compared to the control group. KEGG and GSEA enrichment results indicated that the inhibition of malignant progression in cervical cancer by galangin was significantly associated with the Hippo/YAP signaling pathway. Western Blot confirmed that the expression level of the hallmark protein p-YAP in the Hippo signaling pathway was increased （P < 0.01）, while the expression level of YAP protein was decreased （P < 0.05）. Conclusion Galangin inhibits the proliferation, migration and invasion abilities of Hela cells in a dose-dependent manner. The underlying mechanism might be associated with the activation of the Hippo/YAP signaling pathway.
2025, 46(1): 43-50.
doi: 10.12259/j.issn.2095-610X.S20250106
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Objective To explore the clinical efficacy and safety of immediate laparoscopic radical prostatectomy immediately following the diagnosis of locally advanced prostate cancer. Methods A retrospective analysis was performed for 63 patients with locally advanced prostate cancer who met the inclusion criteria diagnosed in The 1st People's Hospital of Qujing City, Yunnan Province from January 2018 to January 2023. All patients were diagnosed via ultrasound-guided transperineal prostate biopsy. The experimental group consisted of 37 patients who underwent immediate laparoscopic radical prostatectomy after pathological diagnosis, followed by postoperative adjuvant hormone therapy. The control group included 26 patients who first received 3 months of neoadjuvant hormone therapy before undergoing radical surgery, followed by adjuvant hormone therapy postoperatively. The two groups were compared in terms of surgical duration, intraoperative blood loss, length of hospital stay, duration of catheterization, positive surgical margin rate, rectal injury, positive lymph node rate, incidence of urinary fistula and lymphatic leakage, changes in PSA level, postoperative urinary control, biochemical recurrence and distant metastasis. Results In the control group, serum PSA levels, prostate volume, and clinical staging significantly decreased after neoadjuvant hormon therapy. The positive surgical margin rate in the experimental group was significantly higher than that in the control group, with a statistically significant difference（P < 0.05）. There were no statistically significant differences between the two groups in terms of surgical duration, intraoperative blood loss, length of hospital stay, duration of catheterization, rectal injury, positive lymph node rate, incidence of urinary fistula and lymphatic leakage （P > 0.05）. Follow-up at 18 to 36 months revealed no statistically significant differences between the two groups in terms of serum PSA levels at one month post-surgery, complete urinary control rate at six months post-surgery, and biochemical recurrence and distant metastasis at one and two years post-surgery （P > 0.05）. Conclusion Immediate laparoscopic radical prostatectomy following the diagnosis of locally advanced prostate cancer is a clinically safe and feasible treatment option. Noadjuvant hormone therapy reduces tumor staging, lowers the positive surgical margin rate, significantly decreases prostate volume, and provides greater operational space without increasing surgical risks.
2025, 46(1): 51-59.
doi: 10.12259/j.issn.2095-610X.S20250107
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Objective To analysis the efficacy and safety of antiviral therapy in patients with chronic hepatitis B virus in indeterminate phase based on the new guidelines （2022 Edition of the Guidelines for the Prevention and Treatment of Chronic Hepatitis B）. Methods A total of 170 patients with newly diagnosed HBV infection who visited the Third People's Hospital of Kunming from August 1, 2020, to July 31, 2024, were selected as study subjects. The clinical indicators of patients with normal ALT in the indeterminate phase were analyzed after 12 weeks, 24 weeks, and 48 weeks of antiviral treatment, as well as those who did not receive antiviral treatment for 48 weeks. Results （1） Among the 170 patients with normal ALT during the indeterminate phase of HBV infection, the treatment group consisted of 125 patients （36 HBeAg positive and 89 HBeAg negative）, while the untreated group had 45 patients. In the treatment group, the HBV-DNA load and HBsAg titer decreased significantly after 48 weeks compared to before treatment, with statistically significant differences （both P < 0.05）. In the untreated group, the HBV-DNA load showed an upward trend, and the HBsAg titer slightly decreased, with statistically significant differences （both P < 0.05）. （2） The CVR rate in the treatment group after 48 weeks was 66.67% （24/36） for HBeAg positive patients and 95.51% （85/89） for HBeAg negative patients, with a statistically significant difference （P < 0.05）. （3） The treatment group showed a significant decrease in GGT and AFP after 48 weeks compared to before treatment, while the untreated group saw an increase in ALT, GGT, and AFP, with statistically significant differences （all P < 0.05）. （4） The fibrosis indicators APRI, FIB-4, and LSM in the treatment group significantly decreased after 48 weeks compared to before treatment, with statistically significant differences （all P < 0.05）. （5） The safety indicators CREA, blood calcium, and blood phosphorus in the treatment group significantly decreased after 48 weeks compared to before treatment, with statistically significant differences （all P < 0.05）. Conclusion Expanding the antiviral treatment indications according to the new guidelines for patients with normal ALT in the indeterminate phase of HBV infection demonstrates good efficacy in controlling HBV-DNA, improving CVR rates, and enhancing fibrosis indicators, while also showing favorable renal safety. However, there may be a risk of osteoporosis due to calcium and phosphorus metabolism disorders, necessitating enhanced monitoring and prevention.
2025, 46(1): 60-67.
doi: 10.12259/j.issn.2095-610X.S20250108
Abstract:
Objective To understand the current status of stigma among lung cancer patients in Yunnan and analyze its influencing factors. Methods A convenience sampling method was employed to select 248 lung cancer patients from a tertiary cancer hospital in Yunnan from July 2022 to July 2023. A demographic questionnaire and the Chinese version of the Lung Cancer Patient Stigma Scale were used to investigate their demographic characteristics and stigma levels. Results The total score of stigma in lung cancer patients was （96.92 ± 14.87）.Multiple linear regression analysis showed that education level, long-term residence, ethnicity and family relationship were the influencing factors of stigma score in lung cancer patients （P < 0.05）. Conclusion The stigma level among lung cancer patients is relatively high. Patients with higher education levels, those living in rural areas for a long time, ethnic minorities, and those with better family relationships exhibited higher stigma levels. Enhancing the accessibility of medical resources, establishing a comprehensive social support system, and paying attention to and respecting the cultural differences and beliefs of lung cancer patients can provide them with greater understanding, support, and encouragement, thereby reducing the stigma levels.
2025, 46(1): 68-77.
doi: 10.12259/j.issn.2095-610X.S20250109
Abstract:
Objective To explore the influencing factors of self injury or suicide in adolescent patients with depression. Methods A total of 96 adolescents who were diagnosed with depressive disorder at Yunnan Psychiatirc Hospital and Kunming Psychiatirc Hospital from January 2023 to May 2024 were selected for the study. The case group consisted of 61 adolescents who had self-injury or suicidal behaviors within 30 days, while the control group consisted of 35 adolescents who had no self-injury or suicidal behaviors within at least 30 days. Assessments were performed using the Patient Health Questionnaire-9 （PHQ-9）, Millon Screening Inventory for Borderline Personality Disorder （MSI-BPD）, House-Tree-Person Drawing Test （HTP）, and the Columbia Suicide Severity Rating Scale （C-SSRS）. Among these, 72 subjects underwent skin pressure pain threshold testing using the Algometer HP-50, with 12 in the self-harm or suicide group within 2 days and 60 in the group beyond 2 days. Results （1） Analysis of gender and age between the two groups showed statistically significant differences （P < 0.05）, with a higher proportion of females and younger ages in the 30-day group; （2） The PHQ-9 score for the 30-day group was （19.59±5.99）, with a score of （2.41±0.97） for item 9 （"Thoughts that it would be better to be dead or to hurt oneself in some way"）, and an MSI-BPD score of （7.43±1.61）. The scores for the group over 30 days were （10.89±7.99）, （1.00±1.11） for item 9, and （5.40±3.13） for MSI-BPD. Independent samples t-test analysis showed significant differences （P < 0.01）; （3） In the 30-day group, five drawing characteristics from the 47 HTP characteristics （shift of center of gravity, hair not disheveled or no hair, no mountains, no sun, and no flowers or grass） were found to occur at a higher rate, with statistical significance （P < 0.05）; （4） In the binary logistic regression analysis of 96 patients with the 30-day suicide or self-injury behavior as the dependent variable, the absence of flowers and PHQ-9 question 9 score are the risk factors for adolescent depression patients to have self-injury or suicide behavior within 30 days （OR no flowers = 7.934, OR score on PHQ-9 question 9 = 3.554）. （5） Among the 72 subjects the skin pressure pain threshold for adolescents who exhibited self-injure or suicide behaviors within 2 days （8.83±4.27 N） was significantly higher than that for patients beyond 2 days （6.48±3.00 N）, with statistical significance （t = -2.201, P < 0.05）. Additionally, the score for item 9 of the PHQ-9 was higher （2.50±0.67 vs 1.87±0.95）, also showing statistical significance （t = -2.201, P < 0.05）. Conclusion The absence of flowers or grass in HTP painting, and stronger suicidal ideation or suicide attempt are risk factors for self-injury or suicidal behavior in adolescents with depression. Furthermore, those who self-injure or commit suicide within 2 days have a higher pain threshold compared to those beyond 2 days.
GJB4 Gene Expression in Relation to Clinical and Pathological Features of Pancreatic Cancer Patients
2025, 46(1): 78-86.
doi: 10.12259/j.issn.2095-610X.S20250110
Abstract:
Objective To investigate the expression of GJB4 gene in pancreatic cancer tissue and its correlation with clinicopathology. Methods The expression levels of GJB4 mRNA in pancreatic cancer and adjacent cancer tissues were analyzed using bioinformatics to analyze the Cancer Genome Atlas （TCGA） and Genotype-Tissue Expression （GTEx） RNA sequencing datasets. A total of 120 pancreatic cancer samples and 40 adjacent cancer samples from the Pathology Department of The Second Affiliated Hospital of Kunming Medical University from January 2019 to December 2023 were collected and sorted. Immunohistochemistry staining method was used to detect the expression difference of GJB4 protein between the two groups. RT-qPCR method was used to detect the expression levels of GJB4 in four pancreatic cancer cell lines. Univariate and multivariate Cox regression and Kaplan-Meier curves were used to analyze the clinical pathological data of GJB4 and pancreatic cancer patients. DAVID functional annotation bioinformatics and GSEA enrichment analysis were used to explore the relevant pathways of GJB4 in pancreatic cancer. Results The expression level of GJB4 mRNA in pancreatic cancer was higher than that in adjacent tissues, and the high expression of GJB4 was significantly associated with poor prognosis of patients （P < 0.05）. Immunohistochemical analysis showed that GJB4 protein was brown-yellow granular in pancreatic cancer tissues, mainly expressed in cytoplasm and cell membrane, and GJB4 protein expression was up-regulated in pancreatic cancer （P < 0.05）. The RT-qPCR test results showed that out of 4 pancreatic cancer cell lines, 3 of them had upregulated expression （P < 0.05）. COX regression analysis showed that GJB4 gene was an independent risk factor in the prognosis of pancreatic cancer patients. The results of GO enrichment analysis showed that GJB4 was related to the transmembrane transport, ion channel, signal release and membrane potential regulation of pancreatic cancer. GSEA analysis showed that GJB4 was enriched in the Wnt/β-catenin signaling pathway. Conclusion In pancreatic cancer, the high expression level of GJB4 is closely related to the clinicopathological features of the patients, which may predict the poor prognosis of the patients. GJB4 may be involved in regulating the Wnt/β-catenin signaling pathway of pancreatic cancer, and is expected to be one of the potential biomarkers of pancreatic cancer prognosis.
2025, 46(1): 87-92.
doi: 10.12259/j.issn.2095-610X.S20250111
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Objective To investigate the refractive development parameters of primary school students aged 7 to 12 years in Chuxiong City, Yunnan Province, analyze their relationship with equivalent spherical power （SE）, and evaluate the monitoring role of refractive parameters in myopia. Methods A total of 1463 primary school students aged 7-12 from Beipu Primary School in Chuxiong City, Yunnan Province were selected. Spherical degree （DS）, cylindrical degree （DC）, and equivalent spherical degree（SE） were obtained using a computer autorefractor. Axial length （AL）, corneal curvature （K1, K2）, anterior chamber depth （ACD）, central corneal thickness （CCT）, lens thickness （LT）, and vitreous depth （VD） were measured using a biometer. The corneal curvature radius （CR）, the ratio of axial length to corneal curvature radius （AL/CR）, and lens power （LP） were calculated. Participants were divided into 7～8 years old group （n = 518）, 9～10 years old group （n = 547）, and 11～12 years old group （n = 398） according to age; and categorized into myopic group （n = 647）, emmetropic group （n = 532）, and hyperopic group （n = 284） based on refractive status to analyze the characteristics of refractive parameters and their impact across different age groups and refractive categories. Results （1）SE, DS, LT, and LP decreased with age （P < 0.05）; （2）AL, AL/CR, ACD, and VD increased with age （P < 0.05）; （3）K, CR and CCT showed no significant change with age （P > 0.05）; （4）In both myopic and hyperopic groups, AL was negatively correlated with SE （rs = -0.617, rs = -0.318, both P < 0.05）, AL/CR was negatively correlated with SE （rs = -0.737, rs = -0.406, both P < 0.05）, LP was positively correlated with SE （rs = 0.412, rs = 0.182, both P < 0.05）, while CR showed no correlation with SE （P > 0.05）. Conclusion The refractive development parameters AL, AL/CR, ACD, and VD increased with age in primary school students aged 7 to 12, while SE, DS, LT, and LP decreased with age, and K, CR, and CCT showed no significant changes. AL/CR and AL can serve as monitoring indicators for myopia, with AL/CR demonstrating a higher correlation with SE than AL.
2025, 46(1): 93-98.
doi: 10.12259/j.issn.2095-610X.S20250112
Abstract:
Objective To analyze the correlation of preoperative collagen lectin-11 （CL-11）, urinary nitrite, and urinary heparin-binding protein （U-HBP） with postoperative urinary sepsis （US） in patients with kidney stones, and to explore their predictive value and warning significance for postoperative US. Methods A total of 190 patients with kidney stones who underwent minimally invasive percutaneous nephrolithotomy at the Second Affiliated Hospital of Kunming Medical University from September 2021 to June 2023 were selected.According to whether they developed US within 7 days after surgery, they were divided into the US group （n = 30） and the non-US group （n = 160）. Baseline data and preoperative levels CL-11, urinary nitrite and U-HBP were compared between the two groups. The correlation between preoperative CL-11, urinary nitrite, U-HBP and postoperative US was analyzed. The predictive efficacy of preoperative CL-11, urinary nitrite, and U-HBP for postoperative US was evaluated. Results The positive rates of CL-11, urinary nitrite, and U-HBP in the US group were （313.68±38.73） ng/mL, 46.67% and （157.82±41.61） ng/mL, respectively, which were higher than those in the non-US group at （234.00±41.25） ng/mL, 12.50%, and （128.59±36.38） ng/mL （P < 0.05）.Logistic regression analysis indicated that preoperative CL-11, urinary nitrite, and U-HBP were influencing factors for the occurrence of US. The area under the curve （AUC） for predicting postoperative US was 0.797 for CL-11, 0.624 for urinary nitrite, and 0.826 for U-HBP, while the combined AUC for predicting postoperative US was 0.923, with a sensitivity of 90.00% and specificity of 88.75%. Conclusion Patients with postoperative US following kidney stone surgery exhibited elevated levels of urinary nitrite, CL-11, and U-HBP, which were positively correlated with the occurrence of postoperative US. These markers have certain predictive value for postoperative US, and their combined detection offers superior predictive efficacy, potentially serving as warning biomarkers for postoperative US, thereby providing a reference for clinical prevention and control measures.
2025, 46(1): 99-104.
doi: 10.12259/j.issn.2095-610X.S20250113
Abstract:
Objective To measure the Young's modulus values of the quadriceps muscles （rectus femoris, vastus intermedius, vastus lateralis, vastus medialis） and tendons in Parkinson's disease （PD） patients using real-time shear wave elastography （SWE） technology, and to analyze the changes in muscle and tendon tone. Methods A total of 60 clinically diagnosed PD patients from Yunnan University Affiliated Hospital between April 2023 and April 2024 were included in the study group. Based on the scores and ratings of PD rating scale, patients were divided into Group A （symptomatic side, n = 60） and Group B （less symptomatic side, n = 60）. An additional 60 healthy subjects were selected as the control group. The shear wave elastography function of the ACUSON Sequoia ultrasound diagnostic device from Siemens USA was used to measure the Young's modulus values （kPa） of the quadriceps muscles and tendons in a relaxed state, and to analyze and compare the changes in muscle and tendon tone. Results The Young's modulus values of quadriceps muscles （rectus femoris, intermedius femoris, vastus lateralis, vastus medialis） in groups A and B were higher than those in control group （P < 0.05）. The Young's modulus values of the intermediate and lateral femoris muscles in group A were higher than that in group B （all P < 0.05）. The Young's modulus values of quadriceps tendon in groups A and B were higher than that in control group. （P < 0.05）. The Young modulus value of quadriceps tendon in group A was higher than that in group B （P < 0.05）. Conclusion Patients with Parkinson's disease exhibit increased muscle and tendon tone in the quadriceps. Notably, the muscle tone of the vastus intermedius and vastus lateralis on the symptomatic side is higher than that on the less symptomatic side, while there is no significant difference in muscle tone between the rectus femoris and vastus medialis on the symptomatic and less symptomatic sides.
2025, 46(1): 105-110.
doi: 10.12259/j.issn.2095-610X.S20250114
Abstract:
Objective To explore the mediating effect of hope between self-perceived burden and quality of life in patients with recurrent malignant tumors. Methods A cross-sectional survey was conducted using convenience sampling, selecting 204 patients with recurrent malignant tumors from the Oncology Department of Xiangyang Central Hospital in Hubei Province from September to December 2023. A general information questionnaire, Self-Perceived Burden Scale （SPBS）, hope level （HHI）, and EORTC QLQ-C30 quality of life questionnaire were utilized for the investigation. Results The SPBS score for patients with recurrent malignant tumors was （28.28±8.92）, the HHI score was （35.83±4.30） and the quality of life score was （59.68±23.96）. Self-perceived burden was negatively correlated with hope and quality of life （P < 0.05; P < 0.01）, while hope was positively correlated with quality of life （P < 0.01）. The mediating effect of hope between the burden of self-perception and quality of life is reflected, with the indirect effect accounting for 21.18% of the total effect. Conclusion Patients with recurrent malignant tumors generally have a low quality of life and moderate levels of hope, both of which require improvement. The self-perceived burden of patients is at a mild to moderate level. Healthcare professionals should take effective measures to enhance patients' levels of hope and reduce their self-perceived burden, thereby improving the quality of life for patients with recurrent malignant tumors.
2025, 46(1): 111-116.
doi: 10.12259/j.issn.2095-610X.S20250115
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Objective To explore the expression changes of KIAA1199 and Linc00673 genes in non-small cell lung cancer patients before and after chemotherapy, and to further evaluate the potential value of these two genes as molecular markers in predicting chemotherapy efficacy. Methods A total of 58 patients with stage Ⅲ-Ⅳ NSCLC admitted to the Third Hospital of Shijiazhuang and Fengfeng General Hospital of North China Medical Health Group from August 2019 to August 2021 were selected. Peripheral blood PBMCs were collected from patients 2 days before treatment and after two cycles of treatment to detect the expression levels of KIAA1199 and Linc00673 genes. The chemotherapy efficacy of patients was statistically analyzed, and the value of KIAA1199 and Linc00673 genes as molecular markers in predicting chemotherapy efficacy was assessed using ROC curve analysis. Results After 2 cycles of chemotherapy, 20 out of 58 patients showed disease progression, accounting for 34.48%. qPCR results showed no statistically significant difference in the expression levels of KIAA1199 and Linc00673 genes between progressed and non-progressed patients before treatment （P > 0.05）. After treatment, the expression levels of KIAA1199 and Linc00673 genes were significantly lower in non-progressed patients compared to progressed patients （P < 0.01）. ROC curve analysis showed that the AUC of combined detection was significantly higher than that of KIAA1199 gene and Linc00673 gene alone （Z = 3.054, 5.178, P < 0.05）. Conclusion In NSCLC patients undergoing chemotherapy, the expression of KIAA1199 and Linc00673 genes shows significant differences based on therapeutic efficacy, with KIAA1199 being more suitable as a biomarker for predicting chemotherapy efficacy.
2025, 46(1): 117-122.
doi: 10.12259/j.issn.2095-610X.S20250117
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Objective To explore the effects of modified prone ventilation combined with bronchoscopic alveolar lavage on respiratory mechanics and hemodynamics in children with Acute Respiratory Distress Syndrome （ARDS）, as well as to evaluate the clinical treatment efficacy. Methods A total of 96 ARDS children receiving mechanical ventilation treatment in the emergency intensive care unit of Kunming Children's Hospital from January 2021 to December 2023 were selected and randomly assigned into three groups: Group A （prone ventilation group, n = 32 ）, Group B （modified prone ventilation group, n = 32）, and Group C （modified prone ventilation combined with bronchoscopic alveolar lavage group, n = 32）. The changes in the following parameters before and after treatment among the three groups were compared: oxygenation indicators: arterial oxygen partial pressure （PaO2）, arterial oxygenation index （PaO2/FiO2）; respiratory mechanics indicators: lung compliance, mean airway pressure, plateau airway pressure, and total airway resistance; hemodynamic indicators: cardiac output, cardiac index, systemic vascular resistance index, and mean arterial pressure; clinical efficacy indicators time to disappearance of rales, mechanical ventilation duration, and length of hospital stay; and incidence of complications: arrhythmia, airway obstruction, pressure injuries, total incidence of catheter dislodgment, and gastric content reflux. Results The oxygenation indicators in Group C after treatment were superior to those in Groups A and B （P < 0.05）. The respiratory mechanics indicators in Group C after treatment were also better than those in Groups A and B （P < 0.05）. In terms of hemodynamics, there were no statistically significant differences in cardiac output, cardiac index, and mean arterial pressure among Groups A, B, and C after treatment （P > 0.05）. However, the SVRI in Group C was better than that in Groups A and B （P < 0.05）. Curative effect for Group C were also better than those for Groups A and B （P < 0.05）.The incidence of complications in Group C showed no significant difference compared to Groups A and B （P > 0.05）. Conclusion The modified prone ventilation combined with bronchoscopic alveolar lavage treatment scheme demonstrates better therapeutic effects compared to traditional treatment methods, significantly improving oxygenation and respiratory mechanics indicators as well as the systemic vascular resistance index in children, and is worthy of clinical promotion.
2025, 46(1): 123-128.
doi: 10.12259/j.issn.2095-610X.S20250118
Abstract:
Objective To explore the expression changes of IGF-1 and IGFBP-3 in childhood obesity and their diagnostic value. Methods Blood samples and corresponding clinical information were collected from patients attending Kunming Children's Hospital from December 2023 and March 2024, and were divided into a control group （healthy children; n = 115） and a study group （obese children; n = 86）. Serum levels of IGF-1 and IGFBP-3 in both groups were measured by using ELISA. Clinical data from both groups were analyzed to assess the correlation between IGF-1 and IGFBP-3 and clinical information, as well as their diagnostic value in childhood obesity. Results Compared to the control group, the study group exhibited a significant increase in weight and BMI（P < 0.001） while IGF-1 and IGFBP-3 levels were significantly lower （P < 0.05） . IGF-1 levels exhibited a negative correlation with total cholesterol, triglycerides, apolipoprotein B, and insulin resistance index （P < 0.05）, and a positive correlation with apolipoprotein A and fasting insulin （P < 0.05）; IGFBP-3 displayed the opposite pattern （P < 0.05）. ROC diagnostic curves indicated that the area under the curve for IGF-1 and IGFBP-3 was 0.598 and 0.665, respectively. Conclusion IGF-1 and IGFBP-3 are expressed at low levels in childhood obesity and are associated with obesity indicators, demonstrating certain diagnostic value.
2025, 46(1): 129-135.
doi: 10.12259/j.issn.2095-610X.S20250119
Abstract:
Objective To explore the characteristics of the cross-sectional area （CSA） of the vagus nerve in a healthy population in Qujing, Yunnan, and its correlation with demographic characteristics. Methods High-resolution nerve ultrasound examinations were conducted on the vagus nerve of 201 healthy volunteers from a hospital in Qujing, Yunnan from September 2022 to March 2023. The CSA was measured through transverse scans of the vagus nerve. The correlation of vagus nerve CSA with age, body mass index （BMI）, and gender was assessed. Results The mean CSA of the bilateral VN were 1.9 mm2 （right） and 1.3 mm2 （left）, respectively. The average CSA of the right vagus nerve was significantly greater than that of the left （P < 0.001）. In the high-age group （ > 50 years）, the average CSA of the right vagus nerve was significantly higher than that of the low-age group （≤50 years） （P = 0.0142 ）. However, there was no statistically significant difference in the average CSA of the right vagus nerve between the BMI > 24 kg/m2 group and the ≤24 kg/m2 group （P = 0.0852 ）. The average CSA of the left vagus nerve showed no statistically significant differences across different age groups and BMI groups （P > 0.05）, but the average CSA of the bilateral vagus nerve in the male group was greater than that in the female group, with a statistically significant difference （P < 0.05）. Correlation analysis further confirmed a negative correlation between the average CSA of the right vagus nerve and age （r = -0.17, P = 0.0139 ）. Multiple linear regression models indicated that age （P = 0.007） and gender （P = 0.001） were independent influencing factors for the average CSA of the right vagus nerve. Conclusion In the healthy population of Qujing, Yunnan, the CSA of the right vagus nerve is significantly greater than that of the left, and males also exhibit a significantly greater CSA than females. With increasing age, the vagus nerve gradually becomes thinner.
2025, 46(1): 136-141.
doi: 10.12259/j.issn.2095-610X.S20250120
Abstract:
Objective To analyze the expression and diagnostic value of procalcitonin （PCT）, white blood cell count （WBC）, and C-reactive protein （CRP） in respiratory infections in the intensive care unit （ICU）, and their correlation with the clinical pulmonary infection score （CPIS）. Methods A total of 105 patients with respiratory infections admitted to the ICU from March 2019 to June 2024 were selected as the observation group, while 117 patients with no respiratory infection in the ICU were selected as the control group. PCT levels in both groups were measured using the Zybio-Q7 immunoquantitative analyzer, WBC levels were measured using XT-4000i white blood cell analyzer, and CRP levels were measured using the immunoturbidimetric method. The relationship between the expression of PCT, WBC and CRP and the clinical characteristics and CPIs score of respiratory infection in ICU were analyzed. Results The proportion of patients in the observation group with ICU stay > 15 days and mechanical ventilation > 7 days was significantly higher than that in the control group （P < 0.05）. Compared to the control group, the levels of PCT, WBC, CRP and CPIS score in the observation group were elevated, with statistically significant differences （P < 0.05）. Multivariate logistic regression analysis showed that ICU stay > 15 days （OR = 4.087）, PCT （OR = 6.543）, WBC （OR = 2.652）, and CRP （OR = 8.964） were risk factors for the occurrence of respiratory infections in the ICU. Pearson correlation analysis indicated a positive correlation between PCT and CPIS scores （r = 0.925, P = 0.001）, a positive correlation between WBC and CPIS scores （r = 0.739, P = 0.001）; and a positive correlation between CRP and CPIS scores （r = 0.948, P = 0.001）. The ROC curve demonstrated that the combined diagnostic value of the three markers for ICU respiratory infections was higher than that of PCT, WBC, or CRP alone （P = 0.002）. Conclusion The combination of PCT, WBC, and CRP has high diagnostic value for ICU respiratory infections and is positively correlated with CPIs scores. Therefore, the above indicators can provide a reliable basis for the early diagnosis of respiratory infection in ICU.
2025, 46(1): 142-147.
doi: 10.12259/j.issn.2095-610X.S20250121
Abstract:
Objective To investigate the expression and significance of CD4+CD25+ regulatory T cells （CD4+CD25+Treg） and soluble CD30 （sCD30） in peripheral blood of lymphoma patients. Methods A total of 83 lymphoma patients admitted to Beijing Aerospace General Hospital from January 2018 to December 2022 were selected as the research subjects, and their peripheral blood CD4+CD25+Treg and sCD30 expressions were statistically analyzed. Spearman analysis was used to investigate the correlation between peripheral blood CD4+CD25+Treg and sCD30 expressions and bone marrow infiltration. At the same time, the research subjects were divided into an infection group （n = 26） and a non-infection group （n = 57） according to the presence or absence of infection after rituximab chemotherapy. The expressions and differences of peripheral blood CD4+CD25+Treg and sCD30 in the two groups were compared, and the receiver operating characteristic （ROC） curve and area under the curve （AUC） were used to analyze the predictive efficacy. Results The expression of CD4+CD25+Treg and sCD30 in peripheral blood of patients with bone marrow infiltration was higher than that of patients without bone marrow infiltration in the research group （P < 0.05）; the expression of CD4+CD25+Treg and sCD30 in peripheral blood of lymphoma patients was positively correlated with bone marrow infiltration （r = 0.612, 0.634, P < 0.05）; the expression of Tregs and sCD30 in peripheral blood of the infection group after chemotherapy was higher than that of the non-infection group, and the difference was higher than that of the non-infection group （P < 0.05）; the ROC curve showed that the combined prediction of the difference of CD4+CD25+Treg and sCD30 in peripheral blood for the infection after chemotherapy in lymphoma patients had an AUC of 0.916 （0.834～0.965）, which was superior to single prediction. Conclusion The high expression of CD4+CD25+Treg and sCD30 in peripheral blood of lymphoma patients is positively correlated with bone marrow infiltration. Combined testing can improve the predictive efficacy of infection after chemotherapy and guide clinical diagnosis and treatment.
2025, 46(1): 148-153.
doi: 10.12259/j.issn.2095-610X.S20250122
Abstract:
Bacteriophages possess the ability to infect and kill bacteria and have now been applied in various oral diseases, providing new insights for the prevention and treatment of oral diseases. They are expected to become a novel biological antibacterial agent for treating oral diseases. This paper comprehensively discusses the application of bacteriophages in oral medicine from six aspects: the concept and application prospects of bacteriophages, four common infectious diseases of the oral cavity and their pathogenic bacteria, existing treatment methods, and the application and outlook of bacteriophages in these diseases. Lay a theoretical basis for the clinical implementation of phage therapy.
Bacteriophages possess the ability to infect and kill bacteria and have now been applied in various oral diseases, providing new insights for the prevention and treatment of oral diseases. They are expected to become a novel biological antibacterial agent for treating oral diseases. This paper comprehensively discusses the application of bacteriophages in oral medicine from six aspects: the concept and application prospects of bacteriophages, four common infectious diseases of the oral cavity and their pathogenic bacteria, existing treatment methods, and the application and outlook of bacteriophages in these diseases. Lay a theoretical basis for the clinical implementation of phage therapy.
2025, 46(1): 154-159.
doi: 10.12259/j.issn.2095-610X.S20250123
Abstract:
Solitay iliac aneurysms are a rare clinical condition characterized by insidious onset. Once they rupture, treatment becomes difficult, and the mortality rate is extremely high. Therefore, it is extremely important to detect the disease, classify it appropriately and select appropriate treatment options. In recent years, endovascular treatment has gradually replaced traditional laparotomy as the first-line therapeutic approach. Some scholars have proposed clinical classification of isolated iliac aneurysms based on anatomical morphology and imaging studies to better select appropriate endovascular treatment strategies. This review aims to summarize the treatment advancements for solitary iliac aneurysms and the classifications based on the published literature.
Solitay iliac aneurysms are a rare clinical condition characterized by insidious onset. Once they rupture, treatment becomes difficult, and the mortality rate is extremely high. Therefore, it is extremely important to detect the disease, classify it appropriately and select appropriate treatment options. In recent years, endovascular treatment has gradually replaced traditional laparotomy as the first-line therapeutic approach. Some scholars have proposed clinical classification of isolated iliac aneurysms based on anatomical morphology and imaging studies to better select appropriate endovascular treatment strategies. This review aims to summarize the treatment advancements for solitary iliac aneurysms and the classifications based on the published literature.
2025, 46(1): 160-166.
doi: 10.12259/j.issn.2095-610X.S20250124
Abstract:
Objective To explore the impact of the clinical internship model at the on-campus rehabilitation outpatient clinic on the clinical practice abilities and employment intentions of students in the physical therapy program. Methods A total of 60 students from the 2020 cohort of the Rehabilitation Physical Therapy program at Kunming Medical University were selected as research subjects. In the first semester of their sophomore year, they were randomly divided into two groups: the three-phase clinical internship group at the on-campus rehabilitation outpatient clinic （n = 30） and the conventional clinical internship group （n = 30）. The three-phase clinical internship group completed their clinical internship in three phases according to the training objectives at the on-campus rehabilitation outpatient clinic, while the conventional clinical internship group completed their internship according to the requirements of the internship syllabus. At the end of all clinical internships, assessments were conducted using a comprehensive theoretical examination of core professional courses, the Assessment of Physiotherapy Practice （APP）, and an employment intention survey to evaluate the outcomes of the internships for both groups. Results After completing all internship tasks, there was no statistically significant difference in the scores of the comprehensive theoretical examination of core professional courses between the two groups （P > 0.05）. Similarly, there was no statistically significant difference in the total scores of the physiotherapy clinical practice assessment between the two groups （P > 0.05）. However, in the detailed assessments, the three-phase internship group outperformed the conventional group in professional behavior, communication, and evaluation （P < 0.05）. There were no statistically significant differences between the two groups in analysis and planning, intervention, evidence-based practice, and risk management （P > 0.05）. In the employment intention survey, 70% of students in the three-phase internship group expressed a willingness to work in rehabilitation outpatient clinics or clinics, while only 46.6% of the conventional group did so. The comparison of employment intentions between the two groups showed a statistically significant difference （P < 0.05）. Conclusion The three-phase apprenticeship model of the on-campus rehabilitation clinic not only improves students' clinical practice ability, but also increases their confidence in seeking employment in the rehabilitation clinic, thus broadening the employment pathway for physiotherapy students.
2025, 46(1): 167-172.
doi: 10.12259/j.issn.2095-610X.S20250125
Abstract:
Objective To investigate the impact of temperature intervention on pain and catheterization outcomes in premature infants undergoing peripherally inserted central catheter （PICC） placement. Methods A total of 70 premature infants who underwent PICC placement and met the inclusion criteria were selected from the Neonatology Department of a tertiary children's hospital in Kunming, Yunnan, from January 2023 to December 2023. They were randomly divided into a control group （n = 35） and an observation group （n = 35） using a random number table. The control group underwent the standard PICC placement procedure; the observation group underwent temperature intervention measures based on the control group, which included using iodine tincture warmed to 37 ℃ for disinfection, applying warm compresses to the catheterization site with heated hands of the operator, and using saline warmed to 37 ℃ during catheter insertion and skin cleansing. Pain scores measured by the Premature Infant Pain Profile （PIPP）, blood oxygen saturation, heart rate, success rates of single puncture and single catheter insertion, incidence of complications, and occurrence of hypothermia post-catheterization were compared between the two groups. Results The PIPP during disinfection, puncture, catheter insertion, and cleansing were significantly lower in the observation group than in the control group （P < 0.05）; the average blood oxygen saturation in the observation group was higher than that in the control group, with a lower difference compared to the control group （P < 0.05）; the difference and average heart rate during catheterization in the observation group were both lower than those in the control group （P < 0.05）; the success rates of single puncture and single catheter insertion, as well as the incidence of hypothermia post-catheterization in the observation group, were both lower than those in the control group （P < 0.05）, while the comparison of complication rates between the two groups showed no statistically significant difference （P > 0.05）. Conclusion Implementing temperature intervention during PICC placement in premature infants can effectively reduce pain, maintain stable vital signs, improve the success rates of single puncture and catheter insertion, and effectively prevent the incidence of hypothermia.
