Mechanisms of Ginsenoside Rg1 in Protecting Cardiomyocytes by Sestrin2

Ran LIU; Zhi LIN; Fan YANG; Jikun DUAN

doi:10.12259/j.issn.2095-610X.S20230614

Volume 44 Issue 6

Jun. 2023

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Ran LIU, Zhi LIN, Fan YANG, Jikun DUAN. Mechanisms of Ginsenoside Rg1 in Protecting Cardiomyocytes by Sestrin2[J]. Journal of Kunming Medical University, 2023, 44(6): 19-25. doi: 10.12259/j.issn.2095-610X.S20230614

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Ran LIU, Zhi LIN, Fan YANG, Jikun DUAN. Mechanisms of Ginsenoside Rg1 in Protecting Cardiomyocytes by Sestrin2[J]. Journal of Kunming Medical University, 2023, 44(6): 19-25. doi: 10.12259/j.issn.2095-610X.S20230614

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Mechanisms of Ginsenoside Rg1 in Protecting Cardiomyocytes by Sestrin2

doi: 10.12259/j.issn.2095-610X.S20230614

Dept. of Cardiology，The second Affiliated Hospital of Kunming Medical University， Kunming Yunnan 650031，China

Received Date: 2023-03-16
Available Online: 2023-06-19
Publish Date: 2023-06-25

Abstract

Abstract

Objective To explore the role and mechanisms of Sestrin2 in ameliorating hypoxia-reoxygenation injury of rat’s cardiomyocytes by ginsenoside-rg1 （G-Rg1）. Methods Rat H9c2 cardiomyocytes cultured in vitro were randomly divided into 5 groups according to the experimental requirements: control group, Hypoxia reoxygenation group, G-Rg1 group, hypoxia Inducible Factor-1 （HIF-1, HIF-1α） inhibitor group, and Sestrin2 siRNA interference group. Flow cytometry was used to detect the expression levels of ROS in cardiomyocytes. Western blot was used to detect the protein expression of HIF-1α, Sestrin2, ATF-6, PERK, IRE-1 in cardiomyocytes. Immunofluorescence was used to test the protein expression of Sestrin2, HIF-1α, IRE-1. Results Compared with control group, the level of ROS and protein expression of HIF-1α、Sestrin2、ATF-6、PERK、IRE-1 of hypoxiareoxygenation group were significantly increased （P < 0.05） . Compared with the hypoxiareoxygenation group , the ROS level in G-Rg1 group were significantly reduced and protein expression of HIF-1α、Sestrin2、ATF-6、PERK、IRE-1 in G-Rg1 group were significantly increased （P < 0.05） . Compared with G-Rg1 group, the level of ROS were significantly increased and the protein level of Sestrin2 were significantly reduced, in HIF-1α inhibition group （P < 0.05）. Compared with G-Rg1 group, the level of ROS were significantly increased and the protein level of IRE-1 were significantly upregulated in Sestrin2 siRNA group （P < 0.05）. Conclusions G-Rg1 protects rat cardiomyocytes and maintains endoplasmic reticulum homeostasis in vitro by upregulating expression of Sestrin2.
- Ginsenoside-rg1,
- ATF-6,
- PERK,
- IRE-1,
- Sestrin2

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References(20)

References

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