Identification and Culture of Hippocampus Neurons in Fetal Rats

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Identification and Culture of Hippocampus Neurons in Fetal Rats[J]. Journal of Kunming Medical University, 2011, 32(05).

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Identification and Culture of Hippocampus Neurons in Fetal Rats[J]. Journal of Kunming Medical University, 2011, 32(05).

Identification and Culture of Hippocampus Neurons in Fetal Rats[J]. Journal of Kunming Medical University, 2011, 32(05).

Citation:

Identification and Culture of Hippocampus Neurons in Fetal Rats[J]. Journal of Kunming Medical University, 2011, 32(05).

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Identification and Culture of Hippocampus Neurons in Fetal Rats

Abstract

Abstract

Objective To establish the protocol for culturing hippocampus neurons in vitro and explore the neuronal growth character istics.Methods Tissues from hippocampus of fatal rats were harvested,then digested into cell suspension by using 0.125% trypsin.After planted into wells for 2 hours,cell suspension was transferred into next well for continuing incubation.The cell growth was observed at day 5,11 and 15.Neurons specific Enolase(NSE)antibody,recognized specifically NSE antigen in cultured cells,was used to i...
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