Influence of Hypoxia on Hepatoma Carcinoma Cell Line SMMC7721 in Vitro

Article Contents

Article Navigation > Journal of Kunming Medical University > 2011 > 32(05):

Influence of Hypoxia on Hepatoma Carcinoma Cell Line SMMC7721 in Vitro[J]. Journal of Kunming Medical University, 2011, 32(05).

Citation:

Influence of Hypoxia on Hepatoma Carcinoma Cell Line SMMC7721 in Vitro[J]. Journal of Kunming Medical University, 2011, 32(05).

Influence of Hypoxia on Hepatoma Carcinoma Cell Line SMMC7721 in Vitro[J]. Journal of Kunming Medical University, 2011, 32(05).

Citation:

Influence of Hypoxia on Hepatoma Carcinoma Cell Line SMMC7721 in Vitro[J]. Journal of Kunming Medical University, 2011, 32(05).

PDF( 0 KB)

Influence of Hypoxia on Hepatoma Carcinoma Cell Line SMMC7721 in Vitro

Abstract

Abstract

Objective To investigate the effect of hypoxia on human hepatoma carcinoma cell line SMMC7721 cultured in vitro.and to explore the possible molecular mechanism.Methods The chemical method using COCl2 to induce hypoxia environment of SMMC7721 cell was performed.The effect of hypoxia on cell growth,cycle and apoptosis,and adhesion and migration ability were detected by MTT assay,flow cytometry,cell adhesion test,and transwell chamber methods,respectively.RT-PCR and immunocytochemistry methods were used to det...

FullText(HTML)

References(0)

References

Relative Articles(20)

Relative Articles

[1]	Jingjing SONG, Wei XIONG, Shuhui YAO, Shuang LIU, Jing ZHANG. Intervention of AKR1C3 on Malignant Biological Behavior of Breast Cancer Cells through PD1/PD-L1 Signaling Pathway. Journal of Kunming Medical University, 2026, 47(1): 1-9.
[2]	Yongli MO, Xiaoping WEI, Henghai YU, Rui LIU. Effects of p16 on the Cell Cycle and Fatty Acid Metabolism of Gallbladder Cancer Cells. Journal of Kunming Medical University, 2025, 46(12): 1-9.
[3]	Yiran YAN, Chengwan SHEN, Xiangyu SHANG, Chan FENG, Jinqiu LI, Hasim AXIANGU. Galangin Inhibits the Migration and Invasion of Cervical Cancer Hela Cells Through Hippo/YAP Pathway. Journal of Kunming Medical University, 2025, 46(1): 36-42. doi: 10.12259/j.issn.2095-610X.S20250105
[4]	Yanping LI, Xiaolin¹ DONG, Qingyun LI, Hongmei LI, Huan WEI, Yi ZENG. miR-21-5p Alleviates OGD/R-induced Inflammation and Apoptosis of HT22 Cells and Promotes Proliferation by Inhibiting STAT3. Journal of Kunming Medical University, 2024, 45(12): 1-7.
[5]	Xiangchuan QIN, Jinqiu LI, Xiaojing HUANG, Kuerban HUTUBIDING·, Hasim AXIANGU·. Effect of HPV E6 on Proliferation，Invasion and Migration of Cervical Cancer Cells Through Rap1 Signaling Pathway. Journal of Kunming Medical University, 2024, 45(9): 9-16. doi: 10.12259/j.issn.2095-610X.S20240902
[6]	Yuru ZHAI, Yan BAI, Yunyun LI. FGF2 Regulates Hypoxia-Induced Proliferation and Collagen Metabolism of Scleral Fibroblasts Through the PERK/EIF2α/ATF4 Signaling Pathway. Journal of Kunming Medical University, 2024, 45(10): 22-28. doi: 10.12259/j.issn.2095-610X.S20241004
[7]	Liang ZHANG, Baoquan WANG, Xifeng LEI, Xu WANG, Yang KE, Wei ZHANG. Effect of miR-29c-3p/IGF1 Molecular Axis on Activation，Proliferation and Apoptosis of Hepatic Stellate Cells. Journal of Kunming Medical University, 2023, 44(9): 7-14. doi: 10.12259/j.issn.2095-610X.S20230926
[8]	Li ZHANG, Xi ZHAO, Huaxiang ZHAO, Bao XIONG, Jie XU, Guangping TANG, Zhuo YU, Peng CHEN. Effects of Euonymine on Proliferation，Migration，and Cell Cycle of HUVECs and VSMCs. Journal of Kunming Medical University, 2023, 44(7): 1-8. doi: 10.12259/j.issn.2095-610X.S20230723
[9]	Bin ZHAO, Yuanpeng DUAN, Guoying ZHANG, Chengwei BI, Libo YANG, Zhiyu SHI, Yong YANG, Jianpeng ZHANG, Ting GAO. CircRNA EZH2 Promotes Proliferation and Migration of Prostate Cancer Cells by Regulating miR-30c-5p. Journal of Kunming Medical University, 2022, 43(7): 25-32. doi: 10.12259/j.issn.2095-610X.S20220731
[10]	Li ZHANG, Qingcen WANG, Luohui ZHOU, Juan YANG, Hong WANG. Luteolin Inhibits HASMCs Migration by Endoplasmic Reticulum Stress. Journal of Kunming Medical University, 2022, 43(5): 12-17. doi: 10.12259/j.issn.2095-610X.S20220518
[11]	Zhoujun LIAO, Shaohua YANG, Lixin LIU, Sheng HU, Yihui CHEN, Qiang KANG, Xiaowen ZHANG. Effect of AK4 on Proliferation and Migration of Intrahepatic Bile Duct Carcinoma Cell HUCCT1. Journal of Kunming Medical University, 2022, 43(6): 1-6. doi: 10.12259/j.issn.2095-610X.S20220611
[12]	Lan YANG, Xiao JIA, Yi-tong JIANG, Qi CUI, Guang-ci LIU, Ying-hong HE. The Effect of Silencing of UBE2C Gene on the Proliferation and Migration of Human Gastric Cancer AGS Cells. Journal of Kunming Medical University, 2021, 42(5): 18-23. doi: 10.12259/j.issn.2095-610X.S20210504
[13]	Ge Jia Yun . Effects of FHIT Overexpression Mediated by Slow Virus on the growth of Hepatoma Lines in Vitro. Journal of Kunming Medical University,
[14]	Liu Li Xin . . Journal of Kunming Medical University,
[15]	A Yong Jun . . Journal of Kunming Medical University,
[16]	Bai Yu . . Journal of Kunming Medical University,
[17]	Liu Tao . . Journal of Kunming Medical University,
[18]	Shi Zhao Kun . . Journal of Kunming Medical University,
[19]	Liu Jia Xin . . Journal of Kunming Medical University,
[20]	Xu Jun . . Journal of Kunming Medical University,