Diagnostic Value of a Modified Acid-fast Staining Protocol for Mycobacterium Tuberculosis Detection in Pleural Fluid Samples from Patients with Tuberculouspleuritis

Dou Quan Li; Zhou Jiu Peng; Yang Yuan Li; Zhang Yong Feng; Dang Li Yun; Sun Hui Ping

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Article Navigation > Journal of Kunming Medical University > 2017 > 38(09): 35-38

Dou Quan Li , Zhou Jiu Peng , Yang Yuan Li , Zhang Yong Feng , Dang Li Yun , Sun Hui Ping . Diagnostic Value of a Modified Acid-fast Staining Protocol for Mycobacterium Tuberculosis Detection in Pleural Fluid Samples from Patients with Tuberculouspleuritis[J]. Journal of Kunming Medical University, 2017, 38(09): 35-38.

Citation:

Dou Quan Li , Zhou Jiu Peng , Yang Yuan Li , Zhang Yong Feng , Dang Li Yun , Sun Hui Ping . Diagnostic Value of a Modified Acid-fast Staining Protocol for Mycobacterium Tuberculosis Detection in Pleural Fluid Samples from Patients with Tuberculouspleuritis[J]. Journal of Kunming Medical University, 2017, 38(09): 35-38.

Citation:

Dou Quan Li , Zhou Jiu Peng , Yang Yuan Li , Zhang Yong Feng , Dang Li Yun , Sun Hui Ping . Diagnostic Value of a Modified Acid-fast Staining Protocol for Mycobacterium Tuberculosis Detection in Pleural Fluid Samples from Patients with Tuberculouspleuritis[J]. Journal of Kunming Medical University, 2017, 38(09): 35-38.

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Diagnostic Value of a Modified Acid-fast Staining Protocol for Mycobacterium Tuberculosis Detection in Pleural Fluid Samples from Patients with Tuberculouspleuritis

Funds:

基金：陕西省科技惠民计划基金资助项目 (2013K14-02-30);

Received Date: 2017-04-21

Abstract

Abstract

Objective To assess the diagnostic value of a modified acid-fast staining method in tuberculouspleuritis.Methods A modified acid-fast staining protocol was used for Mycobacterium tuberculosis in pleural fluid samples.Detection rates of acid-fast bacilli (AFB) from 151 tuberculous pleural fluid samples were compared between the modified-and traditional Ziehl-Neelsen-acid-fast staining protocols.Results from the BACTEC-MGIT 960 culture system, considered the reference standard, were used to determine sensitivity and specificity of the modified staining method.Results With bacterial culture as the reference standard, sensitivity, specificity, positive predictive value (PPV) , and negative predictive value (NPV) were 72.2%, 100.0%, 100.0%, and 52.9%, respectively, for the modified method, and 0%, 100%, 0%, and 23.8%, respectively, for the traditional method.Conclusions The modified acid-fast staining method has a high detection rate for AFB, and can constitute an important diagnostic tool for tuberculouspleuritis.As a simple, rapid and accurate method, the modified acid-fast staining protocol may provide etiologic evidences for detecting tuberculouspleuritis.
- Modified acid-fast stain,
- Intracellular mycobacterium tuberculosis,
- Tuberculous pleuritis,
- Diagnosis,
- Etiology

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References(17)

References

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