Construction and Identification of Short Hairpin RNA Lentiviral Vector of Human Papillomavirus (HPV) 16 E6

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Construction and Identification of Short Hairpin RNA Lentiviral Vector of Human Papillomavirus (HPV) 16 E6[J]. Journal of Kunming Medical University, 2011, 32(03).

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Construction and Identification of Short Hairpin RNA Lentiviral Vector of Human Papillomavirus (HPV) 16 E6[J]. Journal of Kunming Medical University, 2011, 32(03).

Construction and Identification of Short Hairpin RNA Lentiviral Vector of Human Papillomavirus (HPV) 16 E6[J]. Journal of Kunming Medical University, 2011, 32(03).

Citation:

Construction and Identification of Short Hairpin RNA Lentiviral Vector of Human Papillomavirus (HPV) 16 E6[J]. Journal of Kunming Medical University, 2011, 32(03).

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Construction and Identification of Short Hairpin RNA Lentiviral Vector of Human Papillomavirus (HPV) 16 E6

Abstract

Abstract

Objective To establish a lentiviral vector-mediated RNA interference(RNAi)of Human Papillomavirus(HPV)16 E6 gene.Methods Using genetic recombination technology,a pair of complementary small hairpin RNA(shRNA)oligonucleotides targeting the HPV16 E6 gene was cloned into lentiviral expression vector pGCL-GFP,which contained U6 promoter and green fluorescent protein reporter.The recombined plasmid named pGCL-GFP-vshHPV 16 E6 was confirmed by polymerase chain reaction and sequencing,and was cotransfected with pH...
- E6,

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