TTF-1和PTEN基因在子宫内膜癌早期病变中的表达意义
Significance of Thyroid Transcription Factor-1 and Phosphatase and Tensin Homolog Genes Expression in Early Endometrial Cancer
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摘要: 目的 分析甲状腺转录因子-1 (TTF-1) 和磷酸酶与张力蛋白同源物 (PTEN) 基因在子宫内膜癌早期病变中的表达意义.方法 选择子宫内膜癌41例、增生性子宫内膜38例和正常子宫内膜13例, 采用荧光定量PCR法 (RT-PCR) 检测3种子宫内膜中TTF-1和PTEN mRNA表达量, 分析其与临床病理特征的关系;RT-PCR法检测3种子宫内膜中mi R-135b、mi R-125b和Snail mRNA表达量, 分析其与TTF-1和PTEN mRNA表达量的相关性.结果 子宫内膜癌组织中TTF-1和PTEN mRNA表达量显著低于其他2组, 正常子宫内膜组织中表达量最高, 差异有统计学意义 (P<0.05) .绝经与否、腺癌和鳞癌间TTF-1和PTEN mRNA表达水平比较, 差异无统计学意义 (P>0.05) ;FIGO分期增加、肌层浸润增加、盆腔淋巴结转移的TTF-1和PTEN mRNA表达水平明显降低, 差异有统计学意义 (P<0.05) .子宫内膜癌中mi R-135b mRNA的表达水平显著高于其他2组, 正常子宫内膜中最低;mi R-125b和Snail mRNA的表达水平显著低于其他2组, 正常子宫内膜中最高;差异有统计学意义 (P<0.05) .TTF-1和PTEN mRNA表达量与mi R-135b mRNA表达量呈负相关, 与mi R-125b和Snail mRNA表达量呈正相关 (P<0.05) .ROC模型得出, TTF-1 mRNA诊断子宫内膜癌的敏感性86.5%, 特异性84.2%, 准确性0.823, 95%CI=0.7620.921, P=0.012;PTEN mRNA诊断子宫内膜癌的敏感性85.3%, 特异性83.6%, 准确性0.842, 95%CI=0.7850.936, P=0.010.结论 TTF-1和PTEN基因可作为子宫内膜癌早期诊断的分子标志物, 与临床特征密切相关, 可能通过调控肿瘤细胞增殖活性影响肿瘤进程.Abstract: Objective To study the significance of thyroid transcription factor-1 (TTF-1) and phosphatase and tensin homolog (PTEN) genes expression in early endometrial cancer. Methods A total of 41 consecutives as endometrial cancer, 38 patients with hyperplastic endometrium and 13 cases of normal endometrium were enrolled, then we detected the levels of TTF-1 and PTEN mRNA in the three endometrium tissues by fluorescent quantitative PCR (RT-PCR) , and analyzed the relationship between them and clinicopathological features;the correlation of TTF-1 and PTEN mRNA to mi R-135 b, mi R-125 b and Snail mRNA levels in the three endometrium tissues by RT-PCR. Results The levels of TTF-1 and PTEN mRNA in endometrial cancer tissue were both significantly lower than the other two, and were the highest in normal endometrium (P<0.05) .The expressions of TTF-1 and PTEN were no relationship to menopause, adenocarcinoma or squamous cell carcinoma (P >0.05) ; while they were lower as FIGO classes, myometrial invasion and pelvic lymph node metastasis were increasing (P <0.05) .The level of mi R-135 b mRNA in endometrial cancer tissue was significantly higher, while mi R-125 b and Snail mRNA were lower than the other two, mi R-135 b mRNA level in normal endometrium was the lowest, mi R-125 b and Snail mRNA was the highest (P<0.05) .The TTF-1 and PTEN mRNA expressions were positively ralted to mi R-135 b mRNA level, while negatively related to mi R-125 b and Snail mRNA.The diagnosed sensitivity of TTF-1 mRNA for endometrial cancer was 86.5%, specificity84.2%, accuracy 0.823, 95% CI=0.7620.921, P=0.012 by ROC model;while they were 85.3%, 83.6%, 0.842, 95% CI=0.7850.936, P=0.010 of PTEN mRNA correspondingly. Conclusion TTF-1 and PTEN genes can be the molecular markers for early diagnosis of endometrial cancer, are related to clinical features, and may influence tumor by regulating tumor cell proliferation activity.
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Key words:
- Endometrial cancer /
- Thyroid transcription factor-1 /
- Phosphatase and tensin homolog /
- miR-135b /
- miR-125b /
- Snail
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