Email
RSSCurrent Issue
2025, Volume 46, Issue 10
2025,
46(10):
1-11.
doi: 10.12259/j.issn.2095-610X.S20251001
Abstract:
Prolonged disorders of consciousness （pDoC） are complex and prolonged conditions that severely impact patient prognosis and remain a clinical treatment challenge. In recent years, neural regulation-based awakening therapies have been widely applied in the assessment and treatment of pDoC patients. Repetitive transcranial magnetic stimulation （rTMS） technology can regulate neural activity and improve patients' consciousness states, demonstrating positive awakening effects for pDoC patients. However, the optimal stimulation parameters and awakening mechanisms of rTMS remain unclear. This article reviews the pathological mechanisms of pDoC, clinical applications of rTMS at different targeting sites and stimulation frequencies, and focuses on exploring how rTMS promotes consciousness recovery through neural mechanisms such as altering neural pathways, reshaping brain networks, promoting synaptic plasticity and neurotransmitter release, regulating neurotrophic factor expression, and modulating cerebral hemodynamics. Based on artificial intelligence, the article also prospects the future clinical research applications of rTMS.
Prolonged disorders of consciousness （pDoC） are complex and prolonged conditions that severely impact patient prognosis and remain a clinical treatment challenge. In recent years, neural regulation-based awakening therapies have been widely applied in the assessment and treatment of pDoC patients. Repetitive transcranial magnetic stimulation （rTMS） technology can regulate neural activity and improve patients' consciousness states, demonstrating positive awakening effects for pDoC patients. However, the optimal stimulation parameters and awakening mechanisms of rTMS remain unclear. This article reviews the pathological mechanisms of pDoC, clinical applications of rTMS at different targeting sites and stimulation frequencies, and focuses on exploring how rTMS promotes consciousness recovery through neural mechanisms such as altering neural pathways, reshaping brain networks, promoting synaptic plasticity and neurotransmitter release, regulating neurotrophic factor expression, and modulating cerebral hemodynamics. Based on artificial intelligence, the article also prospects the future clinical research applications of rTMS.
2025,
46(10):
12-22.
doi: 10.12259/j.issn.2095-610X.S20251002
Abstract:
Objective To explore the epidemiological characteristics and treatment outcomes of venomous snake bites in Yunnan Province from 2023 to 2024. Methods A retrospective analysis was performed on 1, 727 snakebite cases collected from 16 administrative regions in Yunnan Province between January 2023 and December 2024, focusing on the following indicators: （1） demographic characteristics （age and gender distribution）; （2） characteristics of injury events （location, time distribution, and affected body parts）; （3） composition of venomous snake species; （4） clinical features and treatment protocols; （5） efficacy evaluation indicators （time for local symptom relief, duration of hospital stay, and follow-up results）. Results High-incidence areas for snake bites in Yunnan Province included Wenshan （308/1727 ）, Kunming （285/1727 ）, Qujing （270/1727 ）, Lincang （139/1727 ）, Baoshan （120/1727 ）, and Dali （105/1727 ）, with incidents primarily occurring from June to September. The most frequently encountered venomous snake species were Ovophis and Trimeresurus, known for their hemotoxic venom. Bite victims were mainly young adults. Clinical manifestations primarily included pain and swelling, with bites mainly occurring on limbs and in mountainous or forested areas. Anti-venom treatment concentrated on anti-ancistrodon acutus serum. Swelling generally subsided within 2 （2, 3） days, with an average hospital stay of 4 （3, 5） days. 96.46% of patients receiving standardized treatment fully recovered, with a follow-up disability rate of 0.35%. Conclusion The incidents of venomous snake bites in Yunnan Province have significant regional and seasonal concentration, with Wenshan, Kunming, Qujing and other areas being high-risk areas. The peak period of incidents is from June to September in summer, and the main types of snakes causing injuries are those containing blood toxins; The victim group is mainly young people, and bites often occur in the limbs, with typical symptoms of pain and swelling; The main treatment is anti snake serum; Snake bite patients who receive formal diagnosis and treatment have a higher recovery rate and a lower follow-up disability rate, but there is still a need to raise public awareness of standardized diagnosis and treatment for snake bites.
2025,
46(10):
23-31.
doi: 10.12259/j.issn.2095-610X.S20251003
Abstract:
Objective To explore the molecular mechanism of miR-16-5p promoting apoptosis and inflammatory response by targeting GPR30 expression in an in vitro ischemic stroke microglia model （BV-2）. Methods An ischemic stroke cell model was established by subjecting BV-2 cells to oxygen-glucose deprivation （OGD）. qRT-PCR was utilized to assess the levels of miR-16-5p and GPR30 mRNA in OGD cells. A miR-16-5p inhibitor was transfected into OGD cells to silence miR-16-5p expression, and alterations in inflammatory response and apoptosis were measured using ELISA kits and Annexin V-FITC/PI staining. Starbase was employed to predict interactions, and dual-luciferase reporter gene assays were conducted to confirm that miR-16-5p targets the 3'-untranslated region （UTR） sequence of GPR30. Changes in cellular inflammatory response and apoptosis were evaluated by knocking down miR-16-5p and/or GPR30 in OGD cells. Results miR-16-5p expression was significantly elevated （P < 0.01）, while GPR30 expression was notably decreased （P < 0.01） in OGD-induced cells. Knockdown of miR-16-5p reduced the expression levels of inflammatory factors and the cell apoptosis ratio （P < 0.01）. Inhibition of miR-16-5p expression led to an upregulation of GPR30 mRNA and protein levels （P < 0.01）. Simultaneous silencing of both miR-16-5p and GPR30 partially enhanced inflammatory factor expression levels and the cell apoptosis ratio compared to cells transfected solely with the miR-16-5p inhibitor （P < 0.05）. Conclusion In the microglia OGD model, miR-16-5p triggers inflammatory responses and enhances apoptosis by inhibiting GPR30 expression.
2025,
46(10):
32-43.
doi: 10.12259/j.issn.2095-610X.S20251004
Abstract:
Objective To investigate the mechanism by which the SLC7A11 gene regulates the development and progression of hepatocellular carcinoma （HCCLM3） through the ferroptosis pathway, and to evaluate its potential as a therapeutic target. Methods Differentially expressed ferroptosis-related genes in liver cancer were screened based on data from the TCGA and ICGC databases. Detection of mRNA expression levels of TERT, MIOX, MYCN, NOX4, and SLC7A11 in tumor and adjacent non-tumorous tissues from 32 clinical liver cancer samples using qRT-PCR. Further analysis of SLC7A11 and its downstream molecules SLC3A2, GSS, and GPX4 was performed through qRT-PCR, Western blot, and IHC to assess expression levels and tissue distribution. A stable SLC7A11-knockdown HCCLM3 cell line was constructed and used to establish a subcutaneous xenograft tumor model in nude mice to evaluate its effect on tumor growth. Mice were divided into two groups （n = 6 per group）: HCCLM3 + sh-NC and HCCLM3 + sh-SLC7A11. Serum levels of IL-6, IL-1β, and TNF-α were measured using ELISA. Histopathological changes in tumor tissues were examined by H&E staining, and the expression of key genes was validated through multiple approaches. Results Bioinformatics analysis showed high expression of SLC7A11 in hepatocellular carcinoma tissues （P < 0.05）, significantly associated with poor patient prognosis. Clinical sample validation revealed significantly higher expression of SLC7A11, SLC3A2, GSS, and GPX4 in cancer tissues compared to control groups （All P < 0.05）. SLC7A11 knockdown significantly inhibited tumor volume and wet weight （P < 0.05）, and H&E staining showed reduced vascular density in the sh-SLC7A11 group. ELISA results showed elevated serum levels of IL-1β, IL-6, and TNF-α in the sh-SLC7A11 group. qRT-PCR, Western blot, and IHC all showed significantly downregulated expression of SLC7A11, SLC3A2, GSS, and GPX4 in tumor tissues （All P < 0.05）. Conclusion SLC7A11 inhibits ferroptosis by regulating the GSH-GPX4 axis, promoting hepatocellular carcinoma cell growth. Targeted inhibition of SLC7A11 can induce tumor cell ferroptosis and suppress tumor progression, suggesting it may be an important therapeutic target for hepatocellular carcinoma.
2025,
46(10):
44-52.
doi: 10.12259/j.issn.2095-610X.S20251005
Abstract:
Objective To investigate if Shikonin （SKI） can induce ferroptosis via the ROS/JNK pathway to inhibit the malignant behavior of pancreatic cancer. Methods Human pancreatic cancer PANC-1 or BxPC-3 cells were selected. Drug efficacy experiments were established with a blank control group （Con group） and low, medium, and high dose SKI groups （2, 4, 8 μmol/L）. JNK-related mechanism experiments were categorized into a blank control group （Con group）, SKI group, and SKI+JNK inhibitor group （SKI+SP600125 group）. ROS-related mechanism experiments were divided into a blank control group （Con group）, SKI group, and SKI+ROS scavenger group （SKI+NAC group）. Cell viability was assessed using the CCK-8 method to calculate IC50; Transwell experiments evaluated cell migration and invasion capabilities; the C11 BODIPY 581/591 probe was utilized for flow cytometry to detect lipid peroxidation levels, while the FerroOrange fluorescent probe measured ferrous ion levels; ROS levels were determined using a ROS detection kit; the Western blot method identified ferroptosis-related key proteins （SLC7A11, GPX4）, apoptosis-related proteins （Caspase3, PARP）, and JNK pathway proteins （JNK, p-JNK）; an in vivo xenograft tumor model was employed to assess tumor proliferation. Results SKI treatment significantly and dose-dependently inhibited PANC-1 cell viability （IC50: 6.04 μmol/L, P < 0.0001 ） and BxPC-3 cell viability （IC50: 12.27 μmol/L, P < 0.0001 ）, and significantly reduced migrating and invasive cell numbers （P < 0.0001 ）, with migration cell numbers dropping to about 30% of the control group at 8 μmol/L SKI treatment （P < 0.0001 ）. Mechanistically, SKI induced increased intracellular lipid peroxidation, Fe2+ accumulation, and significant ROS production （P < 0.0001 ）, significantly downregulated SLC7A11 and GPX4 protein expression （GPX4 protein expression reduced to 40% of that in the control group, P < 0.0001 ）, and activated JNK phosphorylation （p-JNK/JNK ratio increased to 2.8-fold, P < 0.0001 ）. Pretreatment with the JNK-specific inhibitor SP600125 or ROS scavenger NAC effectively reversed SKI's inhibition of cell viability and downregulation of SLC7A11/GPX4 protein （all P < 0.01）. SKI also inhibited pancreatic cancer tumor cell proliferation in vivo （P < 0.0001 ）. Conclusion SKI induces ferroptosis by activating the ROS/JNK pathway, thereby inhibiting pancreatic cancer proliferation, migration, and invasion.
2025,
46(10):
53-60.
doi: 10.12259/j.issn.2095-610X.S20251006
Abstract:
Objective To investigate the regulatory role of miR-147a in affecting cell proliferation and metastasis of cervical cancer Hela cells through ferroptosis. Methods Hela cells were cultured and passaged, and then a Hela single-cell suspension was prepared and transfected. After transfection, the cells were divided into control group, miR-147a mimics NC group, miR-147a mimics group, miR-147a inhibitor NC group, miR-147a inhibitor group, and miR-147a mimics+Ferrostatin-1 （1 μM） group. MTT assay was used to detect cell proliferation, Transwell assay to assess cell invasion capacity, scratch test to evaluate cell healing ability, flow cytometry to detect reactive oxygen species （ROS） expression, and assay kits to measure expression changes of ferrous ions （Fe2+）, ferric ions （Fe3+）, lactoperoxidase （LPO）, glutathione peroxidase （GSH-PX）, and glutathione （GSH）. Results Compared to the control group, the miR-147a mimics group showed reduced cell proliferation rate, cell invasion quantity, and cell migration distance （P < 0.05）; the miR-147a inhibitor group exhibited increased cell proliferation rate, cell invasion quantity, and cell migration distance （P < 0.05）. Compared to the control group, the miR-147a mimics group showed increased Fe2+, LPO, and ROS expression, with decreased Fe3+, GSH-PX, and GSH expression （P < 0.05）. The miR-147a inhibitor group showed decreased Fe2+ , LPO, and ROS expression, with increased Fe3+, GSH-PX, and GSH expression（P < 0.05）. The miR-147a mimics + Ferrostatin-1 group exhibited increased cell proliferation rate, cell invasion quantity, and cell migration distance compared to the miR-147a mimics group （P < 0.05）. Compared to the miR-147a mimics group, the miR-147a mimics + Ferrostatin-1 group showed decreased Fe2+, LPO, and ROS expression levels （P < 0.05）, with increased Fe3+, GSH-PX, and GSH expression levels （P < 0.05）. Conclusion miR-147a can promote ferroptosis and inhibit the proliferation and metastasis of cervical cancer cells.
2025,
46(10):
61-69.
doi: 10.12259/j.issn.2095-610X.S20251007
Abstract:
Objective Analyze the epidemiological characteristics of respiratory virus infections in Kunming area during 2023-2024, and explore the detection rates of different virus types and their distribution patterns across different time periods and age groups. Methods A total of 10354 nasopharyngeal swab or sputum samples were retrospectively collected from patients with acute respiratory infections who visited the Third People's Hospital of Kunming City between March 2023 and June 2024. Multiple pathogens were detected using real-time fluorescent quantitative polymerase chain reaction （RT-qPCR） technology. A retrospective analysis was then conducted on the clinical laboratory detection results, statistically analyzing the overall detection rates of various respiratory viruses, multiple infection phenomena, gender differences, age distribution, seasonal variations, infection site differences, and monthly infection situations. Results Among the 10354 patient respiratory samples tested, 3368 pathogen infections were detected, with a detection rate of 32.53% （3368 /10354 ）. 204 patients presented with mixed infections of ≥2 pathogens, with a mixed detection rate of 6.06%（204/3368 ）. The single detection rate for females was significantly higher than males （P < 0.001）, and the multiple infection detection rate for males is significantly higher than females （P < 0.05）, indicating that males may have a higher risk of concurrent infections. Among different age groups, the virus detection rate was highest in the 5-18 years age group at 55.87%. Significant differences were observed in the detection rates of FluA, FluB, and SIV-H3 across different disease types （P < 0.05）. In March 2023, the detection rate was highest at 54.27% （5619 /10354 ）, with Influenza A virus （FluA） and seasonal influenza H3 subtype （SIV-H3） being the most detected pathogens. Conclusion In acute respiratory infection （ARI） cases in the Kunming area, FluA, FluB, and SIV-H3 were the primary viral pathogens, with the region's viral epidemic characteristics closely related to patient age stages, seasonal changes, and infection site factors.
2025,
46(10):
70-76.
doi: 10.12259/j.issn.2095-610X.S20251008
Abstract:
Objective To compare the efficacy of electroacupuncture in traditional Chinese medicine with the opioid adjuvant drug dezocine. Methods 122 patients undergoing elective laparoscopic cholecystectomy at the Second Affiliated Hospital of Kunming Medical University between October 12, 2023, and April 05, 2024, were randomly allocated into three groups: dezocine group （D group, n = 40）, electroacupuncture group （E group, n = 42）, and electroacupuncture combined with dezocine group （ED group, n = 40）. Patients received 10 mg dezocine, electroacupuncture, or electroacupuncture + 10 mg dezocine after cholecystectomy. Pain threshold index （PTi）, pain index （Pi）, and visual analogue scale （VAS） scores were observed at different time points during surgery. Vital signs were recorded, and adverse reactions within 24 hours postoperatively were noted. Results There were no statistically significant differences in PTi among groups before electroacupuncture （T1） （P > 0.05）. At the end of electroacupuncture （T2） and after cholecystectomy （T3）, the PTi values in the E and ED groups were lower than the D group （P < 0.05）. At the end of surgery （T4） and upon extubation （T5）, the PTi values of all three groups reached a tolerable level for patients, with the E and D groups showing higher PTi values compared to the ED group （P < 0.05）. There were no statistically significant differences in postoperative pain scores （Pi） and VAS at various time points （P > 0.05）. Conclusion Electroacupuncture demonstrates analgesic efficacy non-inferior to opioid drugs and can be recommended as a postoperative pain management technique for laparoscopic cholecystectomy patients.
2025,
46(10):
77-84.
doi: 10.12259/j.issn.2095-610X.S20251009
Abstract:
Objective To investigate the association between serum magnesium levels, serum phosphorus concentrations, vascular calcification, and cardiovascular disease mortality in maintenance hemodialysis patients. Methods This study enrolled 200 hemodialysis patients admitted to Nanjing Drum Tower Hospital from May 2020 to May 2022 as subjects, with an additional 200 healthy individuals from the same period selected as a control group. The biochemical indicators between the two groups were compared; their correlations were analyzed. Binary logistic regression was used to investigate the independent factors of serum magnesium and phosphorus levels in relation to vascular calcification and cardiovascular events in maintenance hemodialysis patients. ROC curve analysis was employed to assess the predictive value of serum magnesium and phosphorus for vascular calcification and cardiovascular events. Results The research group's patients exhibited significantly elevated levels of blood phosphorus, calcium-phosphorus product, iPTH, AACS, and 25-（OH）-VitD compared to the control group. In contrast, their blood magnesium and BMP-7 levels were notably lower than those of the control group, with statistical significance （P < 0.05）. Pearson correlation showed positive correlations between serum magnesium and serum calcium, phosphorus, calcium-phosphorus product, 25-（OH）-VitD3, and BMP-7 （r = 0.385, 0.183, 0.141, 0.131, 0.458, P < 0.05）; between serum calcium and serum phosphorus, calcium-phosphorus product, iPTH, AACS, 25-（OH）-VitD3, and BMP-7 （r = 0.318）, correlation （r = 0.318, 0.311, 0.098, 0.170 , 0.277, 0.485, P < 0.05）; between serum phosphorus and calcium-phosphorus product, iPTH, AACS, 25-（OH）-VitD3 （r = 0.362, 0.506, 0.367, 0.461, P < 0.05）; between calcium-phosphorus product and iPTH, AACS, 25-（OH）-VitD3 （r = 0.542, 0.373, 0.434, P < 0.05）; between iPTH and AACS, 25-（OH）-VitD3 showing positive correlations （r = 0.553, 0.616, P < 0.05） and a negative correlation with BMP-7 （r = -0.373, P < 0.05）; between AACS and 25-（OH）-VitD3 showing a positive correlation （r = 0.402, P < 0.05）, and a negative correlation with BMP-7 （r = -0.155, P < 0.05）, with statistically significant differences （P < 0.05）. Statistically significant differences were noted between the two groups in age, diabetes, serum magnesium, serum calcium, serum phosphorus, calcium-phosphorus product, 25-（OH）-VitD3, and hs-CRP （P < 0.05）. Logistic regression analysis showed that age, serum magnesium, serum calcium, serum phosphorus, calcium phosphate product, 25- （OH） -vitamin I were all risk factors for cardiovascular disease （CVD） mortality in maintenance hemodialysis （MHD） patients （P <0.05）. ROC curve analysis showed that serum magnesium, serum calcium, and serum phosphorus had predictive areas under the curve （AUC） of 0.895, 0.802, and 0.851 for CVD mortality in MHD patients, with sensitivities and specificities of 87.5%/98.7%, 66.7%/90.8%, and 72.9%/100%, respectively. The combined prediction for CVD mortality in MHD patients showed an AUC of 0.921, with a sensitivity of 81.3% and specificity of 93.4%. Conclusion MHD patients exhibit low blood magnesium levels, elevated serum phosphorus concentrations, and increased calcium-phosphorus product, with complex correlations among these biomarkers. Age, magnesium, calcium, and phosphorus levels were all associated with CVD mortality. ROC curve analysis demonstrates that magnesium, calcium, and phosphorus, both individually and in combination, have high predictive value for CVD mortality risk.
2025,
46(10):
85-90.
doi: 10.12259/j.issn.2095-610X.S20251010
Abstract:
Objective To evaluate the predictive value of pyruvate kinase M2（PKM2） and transferrin saturation （TSAT） for major adverse cardiovascular events （MACE） in chronic obstructive pulmonary disease （COPD）- coronary artery disease （CAD） patients after percutaneous coronary intervention （PCI）, and to analyze the relationship between these indicators and patients' cardiac function status. Methods The study cohort included 94 COPD-CAD patients diagnosed in Zhuozhou Hospital from January 2020 to May 2022. Left ventricular end-diastolic volume （LVEDV）, left ventricular end-systolic volume （LVSV）, left ventricular ejection fraction （LVEF）, mitral valve inflow velocity （E） and early diastolic mitral annular velocity （e'） were assessed by echocardiography. Plasma PKM2 and TSAT levels were analyzed using enzyme-linked immunosorbent assay kit. All patients were followed up for 2 years. The study end point was defined as MACE. Results During follow-up, 22 patients （23.4%） experienced MACE. Compared with the non-MACE group, the MACE group showed increased peripheral blood PKM2 levels （P < 0.05） and decreased TSAT levels （P < 0.05）. PKM2 was positively correlated with left ventricular end-systolic volume and E/e' （r = 0.204 and 0.209, both P < 0.05）, and negatively correlated with ejection fraction and TSAT （r = -0.430 and -0.641, both P < 0.001）. TSAT was negatively correlated with left ventricular end-diastolic volume, left ventricular end-systolic volume and E/e' （r = -0.309, -0.470, -0.411, all P < 0.001）, and positively correlated with ejection fraction （r = 0.470, P < 0.001）. PKM2 （Hr = 9.375, 95%CI = 4.145～21.203） and TSAT （Hr = 0.753, 95%CI = 0.645～0.878） were independent influencing factors of MACE events in COPD-CAD patients （P < 0.05）. The AUC of PKM2 and TSAT in predicting MACE events were 0.835 （95%CI = 0.729～0.940） and 0.878 （95%CI = 0.811～0.946）, respectively, with specificity of 72.7% and 90.9%, and sensitivity of 87.5% and 80.6%, respectively. Conclusion In patients with COPD-CAD treated by PCI, the changes of PKM2 and TSAT levels are independently related to MACE events, and are helpful to predict the occurrence of MACE events.
2025,
46(10):
91-96.
doi: 10.12259/j.issn.2095-610X.S20251011
Abstract:
Objective To explore the relationship between serum miRNA-126a, miRNA-130b levels and cognitive impairment （CI） in patients with diabetes mellitus （DM） complicated with acute stroke （AS）. Methods A retrospective analysis was conducted on 70 DM patients with AS admitted to Wuhan First Hospital from January 2019 to December 2021. Patients were divided into CI group （41 cases） and non-CI group （29 cases） based on the occurrence of CI. Real-time fluorescence quantitative PCR was used to detect the relative expression levels of miRNA-126a and miRNA-130b in peripheral blood. Results Compared with non-CI group, the CI group showed increased albumin （P < 0.05） and decreased levels of miRNA-126a and miRNA-130b （P < 0.05）. Logistic regression analysis showed that albumin （OR = 19.045, 95%CI = 2.001～181.230）, miRNA-126a（OR = 200.603, 95%CI = 15.663～2569.162 ）, miRNA-130b （OR = 11.770, 95%CI = 1.966～70.474） were related factors affecting CI occurrence in DM patients with AS. ROC curve analysis showed that peripheral blood miRNA-126a had the highest AUC （0.958） for diagnosing CI in DM patients with AS, with a sensitivity of 97.6% and specificity of 89.7%. The AUC for miRNA-130b （0.669） was lower, with a sensitivity of 73.2% and specificity of 62.1%. Conclusion Decreased expression of peripheral blood miRNA-126a has good diagnostic value for CI in DM patients with AS.
2025,
46(10):
97-105.
doi: 10.12259/j.issn.2095-610X.S20251012
Abstract:
Objective To explore the value of neutrophil extracellular trapping nets （neutrophil extracellular traps, NETs） in the diagnosis and monitoring of acute ischemic stroke （acute ischemic stroke, AIS）. Methods From June 2023 to February 2024, 63 patients newly diagnosed with AIS at the Ganmei Hospital Affiliated with Kunming Medical University were selected as the experimental group, and 58 non-AIS individuals matched in gender, age, and other characteristics were selected as the control group. The NETs levels of patients in the experimental group were detected before and 7-10 days after treatment, and general clinical data and related laboratory results were statistically analyzed. Results The experimental group showed significantly higher levels of NEUT, LYMPH, MONO, EO, FDP, D-dimer, and NETs compared to the control group （P < 0.05）. The NETs levels before treatment were significantly higher than those at 7～10 days after treatment and in the control group （P < 0.05）. The NETs levels 7～10 days after treatment were significantly higher than those in the control group （P < 0.05）. NEUT, MONO, FDP, D-dimer, and NIHSS scores were positively correlated with the pre-treatment NETs levels （P < 0.05）. Elevated serum NETs levels were associated with AIS risk factors （P < 0.05）. The combined diagnostic value of NEUT, D-dimer, and NETs for AIS was superior to other indicators. Conclusion NETs are highly expressed in the serum of patients with acute ischemic stroke, and serum NETs have certain value in the auxiliary diagnosis and monitoring of acute ischemic stroke.
2025,
46(10):
106-112.
doi: 10.12259/j.issn.2095-610X.S20251013
Abstract:
Objective To explore the value of CT combined with MRI （hereinafter referred to as combined diagnosis） in diagnosing sacroiliac joint lesions in ankylosing spondylitis （AS）. Methods A retrospective study selected CT and MRI examination data from 102 AS patients with sacroiliac joint lesions admitted to the Third People's Hospital of Haikou, Hainan Province between January 2021 and July 2023. The study compared the grading and detection rates of various signs using CT, MRI, and combined diagnosis. Results MRI showed higher detection rates for AS sacroiliac joint lesions in grades I-II, bone marrow edema, tendon and ligament attachment point inflammation, subchondral cartilage destruction, synovial thickening, and subchondral fat deposition compared to CT （P < 0.05）. In contrast, CT demonstrated higher accuracy in detecting joint surface sclerosis, subchondral bone erosion/destruction, and joint surface hyperplasia compared to MRI （P < 0.05）. Combined diagnosis showed significantly higher grading and detection rates of AS sacroiliac joint lesion signs compared to MRI or CT alone （P < 0.05）. Conclusion MRI has high detection rates for early AS sacroiliac joint lesions, CT exhibits high sensitivity for joint surface hyperplasia, sclerosis, erosion, and destruction, and combined diagnosis demonstrates superior efficacy compared to MRI or CT individually.
2025,
46(10):
113-120.
doi: 10.12259/j.issn.2095-610X.S20251014
Abstract:
Objective To investigate the early diagnostic value of AI-assisted transvaginal ultrasound combined with serum biomarkers for intrauterine and ectopic combined pregnancy （heterotopic pregnancy, HP） after IVF-ET. Methods This retrospective case-control study included 86 patients diagnosed with HP after IVF-ET at Cangzhou Hospital of Integrated Traditional Chinese and Western Medicine between January 2022 and December 2024. Control groups consisted of 86 patients with normal intrauterine pregnancies and 86 patients with simple ectopic pregnancy. All patients were diagnosed through clinical diagnosis, imaging examinations, and surgical confirmation. An AI ultrasound image analysis model and serum markers （hCG, PAPP-A, APA, progesterone, PlGF） were used to jointly assess HP diagnostic efficiency. Diagnostic performance was compared by calculating sensitivity, specificity, positive predictive value, and negative predictive value. Results The AI ultrasound image analysis model's AUC was 0.835, significantly superior to traditional ultrasound （AUC=0.696）. Serum hCG and PAPP-A levels were （36, 210±9, 820） IU/L and （0.81±0.25） μg/mL, respectively. The joint diagnostic AUC was 0.909, with a sensitivity of 94.2%, specificity of 88.4%, positive predictive value of 91.6%, and negative predictive value of 92.8%. Conclusion AI-assisted ultrasound combined with serological markers significantly improves the early diagnostic accuracy of HP, outperforming single methods and providing a reliable clinical tool.
2025,
46(10):
121-128.
doi: 10.12259/j.issn.2095-610X.S20251015
Abstract:
Objective To explore the influencing factors of liver fibrosis in patients with metabolic dysfunction-associated fatty liver disease （MAFLD） complicated with chronic hepatitis B （CHB）, and to find clinically convenient predictive parameters for assessing liver fibrosis risk. Methods A retrospective analysis was conducted on 221 cases of MAFLD with CHB diagnosed and treated at the Second Hospital of Lanzhou University between January 2015 and August 2022. Patients were divided into low-risk （FIB-4<1.30, n = 84）, medium-risk （1.30≤FIB-4≤2.67, n = 94） and high-risk （FIB-4>2.67, n = 43） liver fibrosis groups based on the Fibrosis-4 （FIB-4） index. General clinical data, laboratory indicators and composite indicators were compared among the three groups. Variables were screened using forward stepwise regression, and binary logistic regression analysis was performed to determine independent predictors of liver fibrosis. A prediction model was constructed and evaluated using receiver operating characteristic （ROC） curve analysis. Results Age, AST and Triglyceride-glucose index（TyG） were independent risk factors for liver fibrosis in patients with MAFLD combined with CHB, while platelet-to-lymphocyte ratio （PLR） was an independent protective factor （all P < 0.05）. ROC curve analysis showed that the area under the curve （AUC） for age, AST, TyG, PLR, age-AST and AST-TyG in predicting liver fibrosis were 0.668, 0.764, 0.680, 0.738, 0.856 and 0.805, respectively. At the optimal cut-off values, the sensitivities were 86.0%, 67.4%, 93.0%, 62.8%, 86.0% and 79.1%, and the specificities were 43.3%, 82.0%, 51.7%, 86.0%, 71.3% and 70.2%. Conclusion Age, AST, TyG and PLR are influencing factors of liver fibrosis in MAFLD combined with CHB patients. The parameters established based on these factors may predict the risk of liver fibrosis, and combined prediction can improve predictive efficacy.
2025,
46(10):
129-136.
doi: 10.12259/j.issn.2095-610X.S20251016
Abstract:
Objective To investigate the effects of early statin therapy on collateral circulation, responsible vessel improvement, and thromboxane B2 （TXB2）/prostaglandin F1 （PGF1α） in acute ischemic stroke with large vessel occlusion （AIS-LVO）. Methods From May 2021 to May 2023, 105 AIS-LVO patients treated with statins within 3 days of admission were selected as the statin group from the Neurology Department of Taiyuan Iron and Steel （Group） General Hospital. Concurrently, 105 early AIS-LVO patients not treated with statins were selected as the non-statin group. The study compared symptom scores [National Institutes of Health Stroke Scale （NIHSS）, modified Rankin Scale （mRS）, Swallowing Standard Assessment （SSA）, and Functional Oral Intake Scale （FOIS） scores], responsible vessel improvement, TXB2/PGF1α levels, and endothelial function indicators [basic fibroblast growth factor （bFGF） and circulating endothelial cells （CECs）]. Results After 1 and 2 weeks , the statin group showed lower NIHSS, mRS, and SSA scores, and higher FOIS scores compared to the non-statin group （P < 0.02）. The statin group demonstrated higher rates of responsible vessel velocity increase, collateral circulation compensation, and superior CTA collateral circulation grading （P < 0.05）. At 1 and 2 weeks, the statin group had lower TXB2 and TXB2/PGF1α levels, and higher PGF1α levels compared to the non-statin group （P < 0.02）. The statin group showed lower bFGF levels and higher CECs levels （P < 0.02）. After 3 months, the statin group had a higher proportion of mRS scores of 0～2 （P < 0.05）. Conclusion Early statin therapy in AIS-LVO patients can improve responsible vessel blood flow and endothelial function, regulate TXB2/PGF1α levels, promote collateral circulation compensation, and enhance swallowing and neurological function recovery.
2025,
46(10):
137-144.
doi: 10.12259/j.issn.2095-610X.S20251017
Abstract:
The risk of post-transfusion hepatitis （PTH） primarily stems from blood transfusion containing HBV-infected blood that is HBsAg-negative, specifically during the serological window period （WP） and occult HBV infection （OBI）. OBI is a low viral replication form of HBV, making it a challenge in hepatitis B virus infection prevention and control, and a significant transmission source of post-transfusion hepatitis. Although nucleic acid testing （NAT） can reduce the occurrence of post-transfusion hepatitis to some extent, some OBI-infected individuals exist among blood donors, representing a potential risk of HBV transmission through blood. This article primarily summarizes an overview of OBI's concept, serological characteristics, genomic features, pathogenesis mechanisms, epidemiological characteristics, and advances in detection strategies, to provide theoretical basis for the collection, quality control and safe use of blood products for the OBI population.
The risk of post-transfusion hepatitis （PTH） primarily stems from blood transfusion containing HBV-infected blood that is HBsAg-negative, specifically during the serological window period （WP） and occult HBV infection （OBI）. OBI is a low viral replication form of HBV, making it a challenge in hepatitis B virus infection prevention and control, and a significant transmission source of post-transfusion hepatitis. Although nucleic acid testing （NAT） can reduce the occurrence of post-transfusion hepatitis to some extent, some OBI-infected individuals exist among blood donors, representing a potential risk of HBV transmission through blood. This article primarily summarizes an overview of OBI's concept, serological characteristics, genomic features, pathogenesis mechanisms, epidemiological characteristics, and advances in detection strategies, to provide theoretical basis for the collection, quality control and safe use of blood products for the OBI population.
2025,
46(10):
145-155.
doi: 10.12259/j.issn.2095-610X.S20251018
Abstract:
Metabolic dysfunction-associated steatotic liver disease （MASLD） has become the most prevalent chronic liver disease worldwide, and China is facing a severe challenge of rapidly increasing MASLD burden. Beverages, as an important modifiable factor, have become a research focus for primary prevention and lifestyle management of MASLD. This article reviews beverage consumption trends, provides an in-depth analysis of the mechanisms and health effects of sugar-sweetened beverages, alcoholic drinks, coffee, and tea on MASLD, summarizes their potential pathogenic and protective pathways, and explores comprehensive strategies including beverage intervention, lifestyle coordination, functional beverage development, psychological and behavioral mechanism regulation, and targeted population prevention. The aim is to provide theoretical basis and practical guidance for the localized and precise prevention and control of MASLD.
Metabolic dysfunction-associated steatotic liver disease （MASLD） has become the most prevalent chronic liver disease worldwide, and China is facing a severe challenge of rapidly increasing MASLD burden. Beverages, as an important modifiable factor, have become a research focus for primary prevention and lifestyle management of MASLD. This article reviews beverage consumption trends, provides an in-depth analysis of the mechanisms and health effects of sugar-sweetened beverages, alcoholic drinks, coffee, and tea on MASLD, summarizes their potential pathogenic and protective pathways, and explores comprehensive strategies including beverage intervention, lifestyle coordination, functional beverage development, psychological and behavioral mechanism regulation, and targeted population prevention. The aim is to provide theoretical basis and practical guidance for the localized and precise prevention and control of MASLD.
2025,
46(10):
156-161.
doi: 10.12259/j.issn.2095-610X.S20251019
Abstract:
Objective To explore the application effects of an innovative teaching model （blended 5MP） that organically combines blended teaching and the five-minute teaching method in clinical teaching for operating room nursing students. Methods Using convenience sampling, 94 nursing students who interned in operating room of Kunming Children's Hospital from July 2023 to March 2024 were randomly assigned to observation and control groups, with 47 students in each group. For the control group, a traditional blended learning approach was utilized, whereas the observation group implemented a blended 5MP teaching model. Results The observation group's post-class assignment scores, theoretical exam scores, and practical skills assessment scores were all significantly higher than those in the control group, with a statistically significant difference （P < 0.05）. The observation group also had higher scores in the Self-Assessment of Clinical Reasoning and Reflection Scale, with a statistically significant difference （P < 0.05）. Furthermore, the observation group reported higher satisfaction rates regarding teaching methods, teaching formats, theoretical instruction, practical instruction, and overall satisfaction compared to the control group, with statistically significant differences（P < 0.05）. Conclusion The blended approach combines online and offline instructional modalities, allowing for a more strategic allocation of educational resources. This methodology enables students to thoroughly acquire essential knowledge and skills while simultaneously fostering advanced clinical reasoning and reflective competencies. By adopting this innovative educational model, institutions can significantly enhance the academic experience and professional development of nursing students.
2025,
46(10):
162-171.
doi: 10.12259/j.issn.2095-610X.S20251020
Abstract:
Objective To assess the level of research integrity awareness among clinical nursing personnel in Yunnan Province, analyze the influencing factors, and identify related risks. Methods From June 2025 to July 2025, a research integrity awareness questionnaire was conducted on 697 clinical nursing personnel from a university-affiliated tumor hospital and children's hospital using the Questionnaire Star platform. The survey content included basic information, research integrity awareness status, research integrity construction awareness, and the causes of research misconduct. Data were analyzed using SPSS 27.0. Results The research integrity awareness score of clinical nursing personnel was （47.49±11.06）, with an overall awareness rate of 51.48%. Univariate analysis showed statistically significant differences in awareness scores based on nursing categories （t = -2.45）, marital status （F = 5.557）, age （F = 2.735）, work experience （F = 4.775）, highest education level （F = 13.11）, whether serving as a master’ s supervisor （t = 2.693）, participation in research projects （t = 4.95）, whether they had published papers as the first author or corresponding author （t = 5.11）, and attendance of research integrity training （t = 12.48） （P < 0.05）. Multivariate linear regression analysis indicated that marital status, highest education level, and participation in research integrity training were significant factors influencing clinical nursing personnel's research integrity awareness （P < 0.05）. The main reason for research misconduct was lack of research integrity awareness The （76.47%）, and 92.37% of clinical nursing personnel believed further strengthening of research integrity construction was necessary. Conclusion The research integrity awareness level of clinical nursing personnel needs improvement. Hospitals should enhance research integrity training, improve research integrity management systems, and optimize scientific talent evaluation mechanisms.
2025,
46(10):
172-178.
doi: 10.12259/j.issn.2095-610X.S20251021
Abstract:
Objective To understand the current status of illness benefit perception among caregivers of type 2 diabetes patients, analyze its relationship with coping strategies and social support, and explore its influencing factors. Methods Using a convenience sampling method, 240 caregivers of type 2 diabetes patients hospitalized in the endocrinology department of a tertiary hospital in Kunming from October 2023 to October 2024 were selected. Questionnaires including general information survey, Benefit Finding Scale, Simplified Coping Strategy Scale, and Social Support Rating Scale were used to investigate the caregivers. Results The caregivers' benefit finding score was （66.82±16.73）, coping strategy score was （36.01±9.64）, and social support score was （42.00±8.11）. Pearson correlation analysis showed positive correlations between coping styles, social support, and illness benefit perception （r = 0.445, r = 0.493, P < 0.01）; multiple linear regression analysis indicated that family residence, education level, duration of caregiving, and understanding of the patient's disease were key factors influencing illness benefit perception（P < 0.01）. Conclusion Caregivers of type 2 diabetes patients have a moderate level of illness benefit perception, with positive correlations between coping strategies, social support, and illness benefit perception. The disease benefit perception of caregivers is also influenced by family residence, education level, caregiving duration, and disease knowledge.
