XXXXXXXXXXXXXX(题目一般不超过25字)

题目格式要求:四号字体，黑体，居中

张三¹⁾，xx²⁾，…(一般不超过6位作者)

（1）昆明医科大学第一附属医院xx科，云南 昆明 650032; 2) xx）

[摘要] 目的 探讨藏红花素（Crocin）在垂体腺瘤（（pituitary adenoma，PA）中的作用及其机制。方法xxx… 结果 RT-qPCR检测及免疫组化显示，与正常对照组相比，PA中IRF7 mRNA的表达增加（P<0.001）；si-IRF7组的IRF7蛋白表达量降低（P<0.001）；CCK-8、Transwell及流式细胞术检测结果显示，与对照组相比，敲低IRF7降低HP75细胞的细胞活力（P<0.001），抑制HP75细胞的迁移和侵袭（P<0.001）…

结论 Crocin处理能抑制PA细胞的增殖、迁移和侵袭，并促进细胞凋亡，缓解PA的发展进程。…

[关键词]xx；xx；xx；xx；xx；xx

[中图分类号] R736.4   [文献标志码]  A

Inhibitory Effect of Crocin on Pituitary Adenomas via IRF7/NF-κB Signaling Pathway

ZHANG¹⁾ San , xx²⁾ ,…

(1)    Department.of xx，The First Affiliated Hospital of Kunming Medical University，Kunming Yunnan 650032; 2)…）

[Abstract] Objective To explore the role and mechanism of crocin in pituitary adenoma (PA). …    Methods xxx …

compared with the control group, knockdown of IRF7 significantly decreased the cell viability of HP75 cells (P<0.001), inhibited the migration and invasion (P<0.001)…

[收稿日期] 2024−08−26

[基金项目] xx（82160513）；xx（202101AY070001-082）

[作者简介] xx（1985～），女，民族（汉族可省略），云南昆明人，医学博士，主治医师，主要从事xx研究工作。

[通信作者]   姓名，E-mail：xxx.

Results RT-qPCR and immunohisto- chemistry showed that compared with the normal control group, the expression of IRF7 mRNA in PA was significantly increased (P<0.001); The expression of IRF7 protein in si-IRF7 group was significantly decreased (P<0.001); CCK-8, Transwell and flow cytometry results showed that compared with the control group, knockdown of IRF7 significantly decreased the cell viability of HP75 cells (P<0.001), inhibited the migration and invasion (P<0.001), …

Conclusions Crocin treatment can inhibit the proliferation, migration and invasion of PA cells, promote cell apoptosis, and alleviate the development of PA.

[Keywords]: xx; xx; xx; xx; xx; xx

正文字体及格式要求：宋体，英文Times New Roman,小四号，1.5倍行距

垂体腺瘤（pituitary adenoma，PA）是起源于垂体前叶的良性肿瘤，根据临床病例研究，其发病率为0.037%~0.116%，约占所有颅内肿瘤的10%^[1-2]。PA常发生在年轻人身上，表现为一系列内分泌症状，…

1 材料与方法

1.1 …

1.2 …

     …

1.X 统计学分析

2 结果

2.1 XX

实验结果显示，…

3 讨论 …

【参考文献】

[1] …