Effect of PTEN Gene Expression on Apoptosis of Thyroid Cancer Cells BCPAP and FTC133 and Expression of ERK and AKT

Jian-wei SUN; Qian XIANG; Zi-chao LIU; Shan-shan XU; Ding DING

doi:10.12259/j.issn.2095-610X.S20210805

Volume 42 Issue 8

Aug. 2021

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Jian-wei SUN, Qian XIANG, Zi-chao LIU, Shan-shan XU, Ding DING. Effect of PTEN Gene Expression on Apoptosis of Thyroid Cancer Cells BCPAP and FTC133 and Expression of ERK and AKT[J]. Journal of Kunming Medical University, 2021, 42(8): 23-30. doi: 10.12259/j.issn.2095-610X.S20210805

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Effect of PTEN Gene Expression on Apoptosis of Thyroid Cancer Cells BCPAP and FTC133 and Expression of ERK and AKT

doi: 10.12259/j.issn.2095-610X.S20210805

1.
Dept. of Ultrasonography
2.
Dept. of Endocrinology，The 5th Affiliated Hospital of Kunming Medical University，Gejiu Yunnan 661000
3.
Dept. of Life Science and Technology， Kunming University，Kunming Yunnan 650214，China

Received Date: 2021-06-15
Available Online: 2021-08-04
Publish Date: 2021-08-04

Abstract

Abstract

Objective To investigate the effect of PTEN gene expression on the apoptosis of BCPAP cells and FTC133 thyroid cancer cells and on the expression of signal pathway proteins ERK and AKT. Methods BCPAP cells and FTC133 cells were transfected with PTEN-pCMV6 and silenced si-PTEN RNA, before the cell microscopic morphology changes were observed. Cell viability was measured by MTT, and apoptosis rate was measured by Annexin V-FITC and PI double staining flow cytometer, respectively. Furthermore, the protein expression levels of ERK, AKT, Bax, Bcl-2, caspase 8, 9, 12 and 3 were detected by Western blot. The association between different expression of PTEN gene and the apoptosis of BCPAP and FTC133 thyroid cancer cells and the expression of signal pathway proteins were analyzed. Results Compared with control group, when the PTEN gene was overexpressed, BCPAP and FTC133 thyroid cancer cells showed microscopic apoptotic changes such as deformation, volume reduction and nuclear pyknosis. The cell viability was decreased （P < 0.01）, and the cell apoptosis rate was increased （P < 0.01）. The expression levels of the apoptotic proteins caspase 8, 9, 12, 3, and proapoptotic protein Bax were significantly increased （P < 0.01）, and the expression of anti-apoptotic protein Bcl-2 was significantly decreased （P < 0.01）, the expression of ERK and AKT were decreased （P < 0.05）. The reduction degree of ERK relative expression in FTC133 cells was significantly higher than that in BCPAP cells （P < 0.01）, while the reduction degree of AKT relative expression in BCPAP and FTC133 cells was not statistically significant （P > 0.05）. After PTEN gene silencing, compared with the control group, the number, cell morphology, volume and nuclear morphology of BCPAP and FTC133 were not significantly different （P > 0.05）. The change of cell viability and apoptosis rate were not significantly different （P > 0.05）. The expression levels of apoptotic proteins caspase 8, 9, 12, 3, and proapoptotic protein Bax was significantly decreased （P < 0.01）, and the expression level of anti-apoptotic protein Bcl-2 were significantly increased （P < 0.01）. The expression levels of ERK and AKT in FTC133 cells were higher than those in the control group （P < 0.01）, and the expression levels of ERK in BCPAP cells were higher than those in the control group （P < 0.01）. Compared with the control group, there was no statistically significant difference in the expression level of AKT （P > 0.05）. The relative expression level of ERK increased in BCPAP and FTC133, but there was no statistically significant difference （P > 0.05）. The relative expression level of AKT increased in FTC133 cells than that in BCPAP cells （P < 0.05）. Conclusion PTEN gene promotes the apoptosis of BCPAP and FTC133 cells. Mitochondrial pathway, death receptor pathway and endoplasmic reticulum activation pathway are all involved in the process of BCPAP and FTC133 cell apoptosis. ERK plays an important role in PTEN gene regulation of BCPAP cell apoptosis, while AKT and ERK both are involved in the process of PTEN gene promoting FTC133 apoptosis.
- PTEN,
- Thyroid cancer,
- Apoptosis,
- ERK,
- AKT

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References(28)

References

[1]	Siegel R L,Miller K D,Jemal A. Cancer statistics,2017[J]. CA Cancer J Clin,2017,67(1):7-30. doi: 10.3322/caac.21387
[2]	刘志春,赵亮. PTEN和Ki-67在甲状腺癌组织中的表达及其临床意义[J]. 中国普外基础与临床杂志,2016,23(11):1348-1352. doi: 10.7507/1007-9424.20160345
[3]	陈超,张伟丽,冯长松. IncRNA PCAT19靶向miR-143-3p通过信号通路PI3K/Akt对甲状腺癌细胞增殖和凋亡的影响及机制[J]. 中国老年学杂志,2020,40(8):1712-1717. doi: 10.3969/j.issn.1005-9202.2020.08.043
[4]	Seong Keun Yoo,Young Shin Song,Eun Kyung Lee,et al. Integrative analysis of genomic and transcriptomic characteristics associated with progression of aggressive thyroid cancer[J]. Nat Commun,2019,10(1):2764-2776. doi: 10.1038/s41467-019-10680-5
[5]	孙健玮. PTEN基因与肿瘤相关性的研究进展[J]. 中国当代医药,2019,26(32):20-23. doi: 10.3969/j.issn.1674-4721.2019.32.007
[6]	Hanzhang Zhu,Qiaoyu Liu,Junwei Tang,et al. Alpha1-ACT functions as a tumour suppressor in hepatocellular carcinoma by inhibiting the PI3K/AKT/mTOR signalling pathway via activation of PTEN[J]. Cell Physiol Biochem,2017,41(6):2289-2306. doi: 10.1159/000475648
[7]	李金国,宋西成. 甲状腺癌相关信号传导通路的研究进展[J]. 肿瘤学杂志,2018,24(4):293-296. doi: 10.11735/j.issn.1671-170X.2018.04.B001
[8]	Jahanbani I,Al Abdallah A,Ali R H,et al. Discriminatory miRNAs for the management of papillary thyroid carcinoma and noninvasive follicular thyroid neoplasms with papillary-like nuclear features[J]. Thyroid,2018,28(3):319-327. doi: 10.1089/thy.2017.0127
[9]	黄敏,倪庆峰. Beclin-1、PTEN蛋白在甲状腺癌中的表达及其意义[J]. 中国普通外科杂志,2017,26(12):1642-1646. doi: 10.3978/j.issn.1005-6947.2017.12.022
[10]	江泽友,徐灿,葛一漫. shRNA干扰IRS-1基因通过PI3K/AKT通路对人乳头状甲状腺癌细胞TPC-1增殖和转移能力的调控作用[J]. 中国免疫学杂志,2018,34(11):1674-1678. doi: 10.3969/j.issn.1000-484X.2018.11.015
[11]	Rodgers S J,Ferguson D T,Mitchell C A,et al. Regulation of PI3K effector signalling in cancer by the phosphoinositide phosphatases[J]. Biosci Rep,2017,37(1):1-18.
[12]	翟明慧,袁殿宝,赵峻峰,等. 过表达PTEN基因对甲状腺髓样癌细胞生长及细胞周期的影响[J]. 临床与病理杂志,2019,39(3):464-469. doi: 10.3978/j.issn.2095-6959.2019.03.002
[13]	Li Za,Qiu Rb,Qiu Xc,et al. EYA2 promotes lung cancer cell proliferation by downregulating the expression of PTEN[J]. Oncotarget,2017,8(67):110837-110848. doi: 10.18632/oncotarget.22860
[14]	孙健玮,郑立民. 抑癌基因PTEN的研究进展[J]. 中国当代医药,2019,26(13):19-22. doi: 10.3969/j.issn.1674-4721.2019.13.006
[15]	陈政华. 甲状腺乳头状癌组织中血管内皮生长因子-C和PTEN蛋白的表达及其与临床病理参数的关系[J]. 中国老年杂志,2016,36(5):1124-1125.
[16]	Beg S,Siraj A K,Jehan Z,et al. PTEN loss is associated with follicular variant of middle eastern papillary thyroid carcinoma[J]. Br J Cancer,2015,112(12):1938-1943. doi: 10.1038/bjc.2015.169
[17]	王振,汪静宇,郭志琴,等. 同源磷酸酶-张力蛋白基因在分化型甲状腺癌组织中的表达及意义[J]. 中国基层医药,2017,24(4):536-539. doi: 10.3760/cma.j.issn.1008-6706.2017.04.014
[18]	于丰铭,徐扬. Caspase-3的研究进展[J]. 中国细胞生物学学报,2020,42(11):2072-2078.
[19]	马汉宁,姬艳燕,陈伟,等. 甘草次酸通过抑制Caspase 3/Bax/Bcl-2凋亡信号通路保护心脏骤停心肺复苏大鼠心脏功能[J]. 中药药理与临床,2019,35(4):28-33.
[20]	Aral Kübra1,Aral Cüneyt Asım,Kapila Yvonne. The role of caspase-8,caspase-9,and apoptosis inducing factor in periodontal disease[J]. J Periodontol,2019,90(3):288-294. doi: 10.1002/JPER.17-0716
[21]	Anderson S L,Townsend HGG,Singh B. Role of toll-like receptor 4 and caspase-3,-8,and-9 in lipopolysaccharide-induced delay of apoptosis in equine neutrophils.[J]. Am J Vet Res,2018,79(4):424-432. doi: 10.2460/ajvr.79.4.424
[22]	Karimi A S,Tafvizi F,Tajabadi E M. Heat-killed probiotic bacteria induce apoptosis of HT-29 human colon adenocarcinoma cell line via the regulation of Bax/Bcl2 and caspases pathway[J]. Hum& Exp Toxicology,2019,38(9):1069-1081.
[23]	王旭同,张永辉. 微小RNA-1269a靶向调控PTEN对肝癌细胞迁移、侵袭和PI3K/Akt信号通路的影响[J]. 临床肿瘤学杂志,2021,26(3):193-199. doi: 10.3969/j.issn.1009-0460.2021.03.001
[24]	G F Zhang,J M Zhong,L Lin. MiR-19 enhances pancreatic cancer progression by targeting PTEN through PI3K/AKT signaling pathway[J]. Eur Rev Med Pharmacol Sci,2020,24(3):1098-1107.
[25]	Jiang Li,Qiao Yanguo,Wang Zhenghui,et al. Inhibition of microRNA-103 attenuates inflammation and endoplasmic reticulum stress in atherosclerosis through disrupting the PTEN-mediated MAPK signaling[J]. J Cell Physiol,2020,235(1):380-393. doi: 10.1002/jcp.28979
[26]	孙健玮,王剑松,刘子超,等. PTEN基因调控Raf1磷酸化对前列腺癌PC3细胞凋亡的影响[J]. 昆明医科大学学报,2020,41(1):18-25. doi: 10.3969/j.issn.1003-4706.2020.01.003
[27]	LeBoeuf B,Anderson B,Young M,et al. Evaluating the effectiveness of MAPK,AKT,and mTOR inhibitors in reducing proliferation in cellular models of papillary and follicular thyroid cancer[J]. Cancer Res,2018,78(13):2887-2888.
[28]	张春英,阴广维,尤鸣达,等. siRNA 靶向沉默TAK1基因对甲状腺癌细胞增殖、迁移和p38 MAPK 信号通路的抑制作用[J]. 吉林大学学报(医学版),2021,47(1):110-117.

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