Serological Study to Self-assembled Nanoparticle Influenza Vaccine with the M2e-HA2 Epitopes

Qi-qi FAN; Heng LI; Xin ZHAO; Jian ZHOU; Lei GUO; Li QIN; Long-ding LIU

doi:10.12259/j.issn.2095-610X.S20210905

Volume 42 Issue 9

Sep. 2021

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Qi-qi FAN, Heng LI, Xin ZHAO, Jian ZHOU, Lei GUO, Li QIN, Long-ding LIU. Serological Study to Self-assembled Nanoparticle Influenza Vaccine with the M2e-HA2 Epitopes[J]. Journal of Kunming Medical University, 2021, 42(9): 20-24. doi: 10.12259/j.issn.2095-610X.S20210905

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Serological Study to Self-assembled Nanoparticle Influenza Vaccine with the M2e-HA2 Epitopes

doi: 10.12259/j.issn.2095-610X.S20210905

Institute of Medical Biology，Chinese Academy of Medical Sciences， Kunming Yunnan 650118，China

Received Date: 2021-07-02
Available Online: 2021-09-03
Publish Date: 2021-09-30

Abstract

Abstract

Objective To construct the self-assembled nanoparticle influenza vaccine with the M2e-HA2 epitopes, and detect the immunological serology. Methods The M2e and HA2 antigen epitopes of influenza viruses were expressed in tandem with Ferritin protein, and the nanometer self-assembled particles were extracted under the condition of non-denatured and purified by affinity chromatography. The mice were immunized for two times, after the two immunizations, serum of mice was collected, western blot was used for antigenicity detection, and ELISA was used for antibody level detection. Results M2e-HA2self-assembled nanoparticles were successfully prepared, and high levels of M2e and HA2 specific antibodies were produced in the serum of immunized mice. Conclusion M2e-HA2 self-assembled nanoparticles can stimulate the production of high levels of M2e and HA2 specific antibodies in mice, laying the foundation for the development of a universal influenza vaccine.
- M2e-HA2 epitopes,
- Self-assembled nanoparticles,
- Influenza vaccine,
- Serological study

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References(21)

References

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