Volume 42 Issue 9
Sep.  2021
Turn off MathJax
Article Contents
Abstract
References
Relative Articles
Article Navigation >  Journal of Kunming Medical University  > 2021  >  42(9): 57-64
Jiang-pin XU, Jun MA, Nan XIE, Qian WU, Fan CAO, Rong-ji YAN, Hua WANG. Clinical Application Value of Ultrasound in the Diagnosis of Acute Appendicitis[J]. Journal of Kunming Medical University, 2020, 41(11): 78-82. doi: 10.12259/j.issn.2095-610X.S20201118
Citation: Jing-hua LIU, Wei CHANG, Fu-bing YU, Juan SHENG, FENG Cheng-cheng, Ni-shan ZHAO. The Expression of C/EBPδ in Colorectal Cancer and Its Effect on the Proliferation of Cancer Cells[J]. Journal of Kunming Medical University, 2021, 42(9): 57-64. doi: 10.12259/j.issn.2095-610X.S20210913
shu

The Expression of C/EBPδ in Colorectal Cancer and Its Effect on the Proliferation of Cancer Cells

doi: 10.12259/j.issn.2095-610X.S20210913
  • 1.

    Dept. of Gastroenterology，The Affiliated Hospital of Yunnan University， Kunming Yunnan 650021

  • 2.

    School of Public Health，Kunming Medical University，Kunming Yunnan 650500，China

  • Received Date: 2021-07-16
    Available Online: 2021-09-09
  • Publish Date: 2021-09-30
    Abstract
  •   Objective  To investigate the expression of C/EBPδ in colorectal cancer and its effect on the proliferation of colorectal cancer cells.  Methods  The expression of C/EBPδ protein in 40 patients undergoing the radical resection of colon cancer in the Second People’ s Hospital of Yunnan province from 2017 to 2018 was detected by immunohistochemistry. The interaction between C/EBPδ and Siah2 in colorectal cancer tissues was detected by immunoprecipitation. Viral vectors with C/EBPδ overexpression and interference were constructed to infect colorectal cancer caco-2 cells, respectively. The effects of C/EBPδ expression and interference on cell proliferation, apoptosis and cycle were observed through CCK8, flow cytometry detection and plate cloning formation experiments. Chi-square test was used to analyze the correlation factors of positive expression rate.  Results  Ⅰ The expression of C/EBPδ increased successively from the normal intestinal tissues to paracellular tissues and then to colorectal cancer tissues, and the differences were statistically significant （all P < 0.05）. The expression of C/EBPδ protein was more significant in colorectal cancer patients with liver metastasis; Ⅱ The C/EBPδ increased colorectal cancer tissues were accompanied by the increase of Saih2, and there was an interaction between the two; Ⅲ C/EBPδ overexpression significantly increased the proliferation of caco-2 cells, while the shRNA interference of C/EBP significantly reduced the proliferation of colorectal cancer cells; Ⅳ Compared with the control group, the shRNA interference of C/EBPδ significantly inhibited the in vitro migration and invasion of colorectal cancer cells caco-2.  Conclusion  C/EBPδ is abnormal in colorectal cancer tissues, and the overexpressed C/EBPδ promotes the invasion and rotation of colorectal cancer cells by interacting with Saih2, which may be related to the poor prognosis of colorectal cancer.
    • FullText(HTML)
    • References(17)
  • [1]
    Feng R M,Zong Y N,Cao S M,et al. Current cancer situation in China:Good or bad news from the 2018 Global Cancer Statistics?[J]. Cancer Commun (Lond),2019,39(1):22.
    [2]
    Richard J,Preen,Larry Bull,Andrew Adamatzky. Towards an evolvable cancer treatment simulator[J]. Biosystems,2019,8(182):1-7.
    [3]
    Qing-Hua Yang,Dong Chen. MiR-567 regulates proliferation and apoptosis of colorecta cancer cells by targeting TRPM8[J]. World Chinese Journal of Digestology,2019,27(14):864-871. doi: 10.11569/wcjd.v27.i14.864
    [4]
    Li,Jing,Zhao Lian-mei,Zhang Cong. The lncRNA FEZF1-AS1 promotes the progression of colorectal cancer through regulating OTX1 and targeting miR-30a-5p[J]. Oncology Research Featuring Preclinical and Clinical Cancer Therapeutics,2020,28(1):51-63. doi: 10.3727/096504019X15619783964700
    [5]
    Xiao-Dong Wang,Jian Lu,Yun-Shou Lin. Functional role of long non-coding RNA CASC19/miR-140-5p/CEMIP axis in colorectal cancer progression in vitro[J]. World Journal of Gastroenterology,2019,25(14):1697-1714. doi: 10.3748/wjg.v25.i14.1697
    [6]
    Wang W,Xia X,Mao L,et al. The CCAAT/Enhancer-Binding protein family:Its roles in MDSC expansion and function[J]. Front Immunol,2019,6(31):1804.
    [7]
    Zhou X,Liu H,Zheng Y,et al. Overcoming radioresistance in tumor therapy by alleviating hypoxia and using the HIF-1 inhibitor[J]. ACS Appl Mater Interfaces,2020,12(4):4231-4240. doi: 10.1021/acsami.9b18633
    [8]
    van Reesema L L S,Zheleva V,Winston J S,et al. SIAH and EGFR,two RAS pathway biomarkers,are highly prognostic in locally advanced and metastatic breast cancer[J]. E Bio Medicine,2016,9(11):183-198.
    [9]
    Amin M B,Greene F L,Edge S B,et al. The eighth edition AJCC cancer staging manual:Continuing to build a bridge from a population-based to a more "personalized" approach to cancer staging[J]. CA Cancer J Clin,2017,67(2):93-99.
    [10]
    Sowamber R,Chehade R,Bitar M,et al. CCAAT/enhancer binding protein delta (C/EBPδ) demonstrates a dichotomous role in tumour initiation and promotion of epithelial carcinoma[J]. EBioMedicine,2019,1(44):261-274.
    [11]
    Tapasree Roy,S. Identification of a Src tyrosine kinase/SIAH2 E3 ubiquitin ligase pathway that regulates C/EBPδ expression and contributes to transformation of breast tumor cells[J]. Molecular and Cellular Biology,2012,32(2):320-332. doi: 10.1128/MCB.05790-11
    [12]
    JanWillem D,Lin C,Moog S,et al. CCAAT/enhancer binding protein delta (C/EBPδ) deficiency does not affect bleomycin-induced pulmonary fibrosis[J]. J Clin Transl Res,2018,3(Suppl 2):358-365.
    [13]
    Natalya N Pavlova,Craig B Thompson. The emerging hallmarks of cancer metabolism[J]. Cell Metab,2016,23(1):27-47. doi: 10.1016/j.cmet.2015.12.006
    [14]
    Albadari N,Deng S,Li W. The transcriptional factors HIF-1 and HIF-2 and their novel inhibitors in cancer therapy[J]. Expert Opin Drug Discov,2019,14(7):667-682. doi: 10.1080/17460441.2019.1613370
    [15]
    Kachamakova-Trojanowska N,Podkalicka P,Bogacz T,et al. HIF-1 stabilization exerts anticancer effects in breast cancer cells in vitro and in vivo[J]. Biochem Pharmacol,2020,175(20):113922.
    [16]
    Sinem Karaman,Veli-Matti Leppänen,Kari Alitalo. Vascular endothelial growth factor signaling in development and disease[J]. Development,2018,145(14):dev151019. doi: 10.1242/dev.151019
    [17]
    Pezzuto A,Carico E. Role of HIF-1 in cancer progression:Novel insights. A review[J]. Curr Mol Med,2018,18(6):343-351.
    • Relative Articles(20)

  • Relative Articles

    [1] Bo LIU, Qiaolian WU, Quanchang ZHANG. The Effects of the Laparoscopic and Open Surgery on the Postoperative Intestinal Function Recovery Time in Elderly Patients with Colorectal Cancer. Journal of Kunming Medical University, 2024, 45(1): 143-148.  doi: 10.12259/j.issn.2095-610X.S20240124
    [2] Zhaoyang CAO, Sijia LIU, Yaying YANG. Advancements of Radiomics in Coloractal Cancer. Journal of Kunming Medical University, 2024, 44(1B): 1-7.
    [3] Yuan WANG, Rong LI, Jiaojiao CHEN, Yanli SHEN, Fengdi HU. Clinical Value of Fecal SDC2 Gene Methylation Detection in Early Screening of Colorectal Cancer. Journal of Kunming Medical University, 2023, 44(5): 138-143.  doi: 10.12259/j.issn.2095-610X.S20230524
    [4] Haimei GOU, Li FANG, Xiaowu ZHONG. Relationship between Mutations of KRAS，NRAS and BRAF Genes and Colorectal Liver Metastases. Journal of Kunming Medical University, 2023, 44(1): 19-24.  doi: 10.12259/j.issn.2095-610X.S20230106
    [5] Liju ZHANG, Xiaoming JIANG, Changxian CHEN, Xi WU, Zhenyong ZHANG, Weijun LIU. Long Non-coding RNA-p21 Regulates the MicroRNA-9 / Sirtuin-1 Signaling Pathway to Reverse Oxaliplatin Resistance in Colorectal Cancer Cells. Journal of Kunming Medical University, 2022, 43(5): 27-32.  doi: 10.12259/j.issn.2095-610X.S20220519
    [6] Helong CHE, Zhijin LI, Keqiang WANG, Weiming QIU, Yi CAO, Laibin LUO. Effect Analysis of Emergency Endoscopic Treatment for Anastomotic Hemorrhage after Colorectal Cancer Resection. Journal of Kunming Medical University, 2022, 43(10): 122-126.  doi: 10.12259/j.issn.2095-610X.S20221032
    [7] Yubin LE, Shikui GUO. The Value of Serum Adipokine in the Long-term Prognosis of Colorectal Cancer Patients. Journal of Kunming Medical University, 2022, 43(4): 82-88.  doi: 10.12259/j.issn.2095-610X.S20220417
    [8] Zi-hong CHEN, Shi-bo SUN, Xian-mei WANG, Shan-shan YU, Xiu-fang WU, Xiang-shan CHEN, Ting GENG, Hong LIU, Xian-min LIU, Qiong NAN. Abnormal Coagulation Parameters in Patients with Early Colorectal Cancer and Its Clinical Significance. Journal of Kunming Medical University, 2021, 42(5): 96-100.  doi: 10.12259/j.issn.2095-610X.S20210518
    [9] Deng Lan Ying , Peng Pai Lan , Liu Yan Ni . . Journal of Kunming Medical University, 2020, 41(09): 125-131.
    [10] Yang Huai Yong , Li Lin Da . . Journal of Kunming Medical University, 2020, 41(04): 111-116.
    [11] Yang Jing Yu , Li Wen Liang . . Journal of Kunming Medical University, 2020, 41(04): 150-156.
    [12] Yang Hong Jun , Wang Xiao Bin , Sun Xiang Hua , Li Zhi Gang . . Journal of Kunming Medical University, 2020, 41(06): 105-108.
    [13] Zou Si Si , Song Xiao Yan , Deng Fei , Xu Ji Zong . . Journal of Kunming Medical University, 2019, 40(11): 92-96.
    [14] Zhao Min , Zhang Qiang , Ma Yong Ci , Long Fei , Lin Yan Ping , Xu Xue Bin , Zhang Jin Ping . A Clinical Features Analysis of 1 000 Cases of Colorectal Cancer in Yunnan Tumor Hospital. Journal of Kunming Medical University, 2018, 39(05): 90-93.
    [15] Wu Xue Song . . Journal of Kunming Medical University,
    [16] Yang Jian Hua . . Journal of Kunming Medical University,
    [17] Wang Ruo Tian . . Journal of Kunming Medical University,
    [18] Zhong Lin . . Journal of Kunming Medical University,
    [19] Xiao Yun . . Journal of Kunming Medical University,
    [20] Yang Yi Ming . Clinical Experiences in Diagnosis and Treatment of Intestinal Obstruction of 48 Patients with Colorectal Cancer. Journal of Kunming Medical University,
    • Supplements(0)
    • Cited By

  • Cited by

    Periodical cited type(3)

    1. 张兴平，周轶平，缪晶茜，李碧桃，文政琦，王扣. 喙尾琵琶甲化学成分的研究. 中草药. 2025(04): 1130-1137 .
    2. 赵敏，杨于杰，郭芳芳，赵旭鸽，陈利军，尹健，徐运飞. HS-SPME-GC-MS分析钩臀蚁蛉幼虫挥发性成分. 信阳农林学院学报. 2024(01): 101-104+109 .
    3. 闫靖楠，马科，吴丹，李秀育，刘文杰，张春姣，鲍素乐. 臭壳虫化学成分和药理作用研究概况及质量标志物的预测分析. 中药药理与临床. 2024(08): 108-114 .

    Other cited types(0)

  • Created with Highcharts 5.0.7Amount of accessChart context menuAbstract Views, HTML Views, PDF Downloads StatisticsAbstract ViewsHTML ViewsPDF Downloads2024-052024-062024-072024-082024-092024-102024-112024-122025-012025-022025-032025-040
    Created with Highcharts 5.0.7Chart context menuAccess Class DistributionFULLTEXT: 34.0 %FULLTEXT: 34.0 %META: 65.5 %META: 65.5 %PDF: 0.5 %PDF: 0.5 %FULLTEXTMETAPDF
    Created with Highcharts 5.0.7Chart context menuAccess Area Distribution其他: 1.9 %其他: 1.9 %其他: 0.1 %其他: 0.1 %Beauharnois: 0.3 %Beauharnois: 0.3 %Falkenstein: 0.2 %Falkenstein: 0.2 %Seattle: 0.1 %Seattle: 0.1 %上海: 0.6 %上海: 0.6 %东莞: 0.2 %东莞: 0.2 %伊利诺伊州: 0.1 %伊利诺伊州: 0.1 %佛山: 0.2 %佛山: 0.2 %保定: 0.1 %保定: 0.1 %六安: 0.1 %六安: 0.1 %北京: 70.0 %北京: 70.0 %十堰: 0.2 %十堰: 0.2 %南京: 0.1 %南京: 0.1 %南通: 0.1 %南通: 0.1 %哥伦布: 0.1 %哥伦布: 0.1 %嘉兴: 0.1 %嘉兴: 0.1 %天津: 0.4 %天津: 0.4 %太原: 0.1 %太原: 0.1 %宣城: 0.3 %宣城: 0.3 %常德: 0.1 %常德: 0.1 %平顶山: 0.1 %平顶山: 0.1 %张家口: 1.0 %张家口: 1.0 %德罕: 0.1 %德罕: 0.1 %成都: 0.2 %成都: 0.2 %扬州: 0.3 %扬州: 0.3 %拜仁: 0.2 %拜仁: 0.2 %昆明: 0.3 %昆明: 0.3 %朝阳: 0.1 %朝阳: 0.1 %杭州: 0.3 %杭州: 0.3 %泉州: 0.1 %泉州: 0.1 %济南: 0.1 %济南: 0.1 %淮北: 0.1 %淮北: 0.1 %深圳: 0.5 %深圳: 0.5 %温州: 0.4 %温州: 0.4 %漯河: 0.9 %漯河: 0.9 %玉溪: 0.1 %玉溪: 0.1 %纽约州: 0.1 %纽约州: 0.1 %芒廷维尤: 11.9 %芒廷维尤: 11.9 %芝加哥: 1.0 %芝加哥: 1.0 %西宁: 5.6 %西宁: 5.6 %西安: 0.1 %西安: 0.1 %贵阳: 0.1 %贵阳: 0.1 %赣州: 0.1 %赣州: 0.1 %运城: 0.1 %运城: 0.1 %遵义: 0.1 %遵义: 0.1 %邯郸: 0.1 %邯郸: 0.1 %郑州: 0.3 %郑州: 0.3 %重庆: 0.6 %重庆: 0.6 %金华: 0.1 %金华: 0.1 %长沙: 0.1 %长沙: 0.1 %马鞍山: 0.1 %马鞍山: 0.1 %驻马店: 0.1 %驻马店: 0.1 %其他其他BeauharnoisFalkensteinSeattle上海东莞伊利诺伊州佛山保定六安北京十堰南京南通哥伦布嘉兴天津太原宣城常德平顶山张家口德罕成都扬州拜仁昆明朝阳杭州泉州济南淮北深圳温州漯河玉溪纽约州芒廷维尤芝加哥西宁西安贵阳赣州运城遵义邯郸郑州重庆金华长沙马鞍山驻马店

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Figures(5)  / Tables(3)

    Get Citation
    PDF
    XML

    Article Metrics

    Article views (3807) PDF downloads(22) Cited by(3)
    Proportional views
    Related

    Copyright © 《昆明医科大学学报》编辑部  滇ICP备00000000号-0

    Publishing: 昆明医科大学学报编辑部

    Supported by: Beijing Renhe Information Technology Co. Ltd    

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return