The Effect of Hydrogen-Regulated Autophagy on Pulmonary Fibrosis in Paraquat Poisoned Rats

Yong-jun LIU; Yin WANG; Mei YANG; E-gao YIN; Ting LI; Zhao-xing DONG

doi:10.12259/j.issn.2095-610X.S20210918

Volume 42 Issue 9

Sep. 2021

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Yong-jun LIU, Yin WANG, Mei YANG, E-gao YIN, Ting LI, Zhao-xing DONG. The Effect of Hydrogen-Regulated Autophagy on Pulmonary Fibrosis in Paraquat Poisoned Rats[J]. Journal of Kunming Medical University, 2021, 42(9): 1-6. doi: 10.12259/j.issn.2095-610X.S20210918

Citation:

Yong-jun LIU, Yin WANG, Mei YANG, E-gao YIN, Ting LI, Zhao-xing DONG. The Effect of Hydrogen-Regulated Autophagy on Pulmonary Fibrosis in Paraquat Poisoned Rats[J]. Journal of Kunming Medical University, 2021, 42(9): 1-6. doi: 10.12259/j.issn.2095-610X.S20210918

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The Effect of Hydrogen-Regulated Autophagy on Pulmonary Fibrosis in Paraquat Poisoned Rats

doi: 10.12259/j.issn.2095-610X.S20210918

1.
Dept of Respiratory and Critical Care，The 2nd Affiliated Hospital of Kunming Medical University，Kunming Yunnan 650101
2.
Hospital of Yunnan Normal University， Kunming Yunnan 650101
3.
University of Chinese Academy of Sciences，Ningbo Huamei Hospital, Ningbo Zhejiang 315000，China

Received Date: 2021-06-15
Available Online: 2021-09-27
Publish Date: 2021-09-25

Abstract

Abstract

Objective To investigate the therapeutic effect and mechanism of hydrogen water on paraquat induced pulmonary fibrosis in rats through autophagy. Methods 6-8 week=old SD rats were randomly divided into the control group, the paraquat exposure group, the hydrogen-water treatment group, the rapamycin treatment group and the comprehensive treatment group （10 rats in each group）. All rats were sacrificed after 28 days and their lung tissues were taken and stained with hematoxylin & eosin as well as Masson for histological examination. The expressions of Col-I and COL-III mRNA were detected by RT-PCR, and the expressions of P62 and LC3 were detected by Western Blot method. Results Compared with the control group, lung tissue of rats exposed to paraquat showed pathological changes of fibrosis, increased Col-ImRNA and Col-IIImRNA expression （P < 0.01）, increased P62 expression （P < 0.001）, and decreased LC3II/I value （P < 0.05）. Compared with the paraquat exposure group, the degree of pulmonary fibrosis in hydrogen water treatment group was reduced, and the expressions of Col-ImRNA and Col-IIImRNA were decreased （P < 0.05）, the expression of P62 was decreased （P < 0.05）, and the LC3II/I value was increased （P < 0.05）. Compared with the paraquat exposure group, the degree of pulmonary fibrosis in rapamycin treatment group was reduced, the expression of Col-ImRNA and Col-IIImRNA were decreased （P < 0.05）, the expression of P62 was decreased （P < 0.01）, and the LC3II/I value was increased （P < 0.01）. Compared with the paraquat poisoning group, the degree of pulmonary fibrosis in the comprehensive treatment group was significantly reduced, the expressions of Col-ImRNA and Col-IIImRNA were significantly decreased （P < 0.01）, the expression of P62 was significantly decreased （P < 0.01）, and the LC3II/I value was significantly increased （P < 0.001）. Conclusion Paraquat induces the pulmonary fibrosis and inhibites the autophagy in rats; while Rapamycin activates the autophagy and alleviates the paraquat-induced pulmonary fibrosis in rats. Hydrogen water alleviates the paraquat-induced pulmonary fibrosis and promotes the autophagy in rats. Hydrogen water combined with rapamycin is better than hydrogen water combined with rapamycin alone in the treatment of paraquat induced pulmonary fibrosis.
- Pulmonary fibrosis,
- Paraquat,
- Hydrogen water,
- Autophagy

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