The Detection Results of Viral Nucleic Acid in Various Biological Samples of Patients with COVID-19 in Kunming

Dong-ling LI; Li-hua LI; Dong PU; Hong-ying WANG; Ting-ting YU; Jing BAI

doi:10.12259/j.issn.2095-610X.S20220106

Article Contents

Article Navigation > Journal of Kunming Medical University > 2022 > Accepted Manuscript

Dong-ling LI, Li-hua LI, Dong PU, Hong-ying WANG, Ting-ting YU, Jing BAI. The Detection Results of Viral Nucleic Acid in Various Biological Samples of Patients with COVID-19 in Kunming[J]. Journal of Kunming Medical University. doi: 10.12259/j.issn.2095-610X.S20220106

Citation:

Dong-ling LI, Li-hua LI, Dong PU, Hong-ying WANG, Ting-ting YU, Jing BAI. The Detection Results of Viral Nucleic Acid in Various Biological Samples of Patients with COVID-19 in Kunming[J]. Journal of Kunming Medical University. doi: 10.12259/j.issn.2095-610X.S20220106

Citation:

Dong-ling LI, Li-hua LI, Dong PU, Hong-ying WANG, Ting-ting YU, Jing BAI. The Detection Results of Viral Nucleic Acid in Various Biological Samples of Patients with COVID-19 in Kunming[J]. Journal of Kunming Medical University. doi: 10.12259/j.issn.2095-610X.S20220106

PDF( 774 KB)

The Detection Results of Viral Nucleic Acid in Various Biological Samples of Patients with COVID-19 in Kunming

doi: 10.12259/j.issn.2095-610X.S20220106

Dept. of laboratory，The Third People's Hospital of Kunming，Kunming Yunnan 650041，China

Received Date: 2021-12-16
Available Online: 2021-12-20

Abstract

Abstract

Objective To detect the viral status of SARS-CoV-2 nucleic acid in various biological samples of patients diagnosed with New Coronavirus pneumonia （COVID-19） by using the nucleic acid reagents of New Coronavirus （2019-nCoV）. The status and clinical significance of SARS-CoV-2 in different body systems were observed, providing reference for diagnosis and treatment. Methods The nasal swabs, throat swabs, sputum, feces and urine samples of 53 patients diagnosed with New Coronavirus lung （COVID-19） in Kunming Third People's Hospital were collected. Real-time fluorescence reverse transcription polymerase chain reaction （RT-PCR） was used to detect the ORF1ab gene and N gene nucleic acid in parallel. The positive rates and consistency of different samples were compared. Results The results showed that the changes of CT value of nasal swab, pharyngeal swab, sputum sample and fecal sample were highly correlated with the course of disease. The analysis of nucleic acid detection results 15 days before and after the onset showed that the difference of CT value of viral nucleic acid 15 days after the onset was statistically significant compared with that within 15 days （P < 0.05）. The positive rate of nasal swab was 88.67% （47/53） and 79.24% （42/53）, while that of pharyngeal swab was 73.58% （39/53） and 53.85% （28/52）. The positive rate of nucleic acid detection in stool samples was 12.28% （7/57）, and no viral nucleic acid was detected in urine samples. Conclusion In novel coronavirus pneumonia patients at different stages of onset, nasal swabs, throat swabs and sputum samples show higher nucleic acid detection rates. The positive rate of nasal swab is higher than that of pharyngeal swab. SARS-CoV-2 has the possibility of fecal oral transmission, and the digestive tract may be the potential target organ of virus infection.

FullText(HTML)

References(21)

References

[1]	Du R H,Liang L R,Yang C Q,et al. Predictors of mortality for pa- tients with COVID-19 pneumonia caused by SARS-CoV-2:aprospective cohort study[J]. Eur Respir J,2020,55(5):2000524. doi: 10.1183/13993003.00524-2020
[2]	Jin X,Lian J S,Hu J H,et al. Epidemiological,clinical and virological characteristics of 74 cases of coronavirus-infected disease 2019 (COVID-19) with gastrointestinal symptoms[J]. Gut,2020,69(6):1002-1009. doi: 10.1136/gutjnl-2020-320926
[3]	Zhou F,Yu T,Du R,et al. Clinical course and risk factors for mortality of adult inpatients with COVID-19 in Wuha,China:A retrospective cohort study[J]. Lancet,2020,395(10229):1054-1062. doi: 10.1016/S0140-6736(20)30566-3
[4]	WHO coronavirus disease（COVID-19） dashboard[Z]. 2020. https://covid19.who.int
[5]	国家卫生健康委. 新型冠状病毒感染的肺炎诊疗方案（试行第八版修订版）国家卫生健康委（2020-4-14）[EB/OL] .http://www.nhc.gov.cn/xcs/zhengcwj/202008/0a7bdf12bd4b46e5bd28ca7f9a7f5e5a/files/a449a3e2e2c94d9a856d5faea2ff0f94.pdf
[6]	中华人民共和国国家卫生健康委员会 . 《新型冠状病毒感染的肺炎诊疗方案（试行第七版）》（2020-03-03）[EB/OL]. http://www. nhc. gov. cn/yzygj/s7653p/202003/46c9294a7dfe4cef80dc7f5912eb1989.shtml.
[7]	国家卫生部办公厅, 国家卫生健康委员会医政医管局办公室. 医疗机构临床基因扩增检验实验室管理办法[EB/OL]. （2010-12-06）. https://www.51mch.com/uploadfiles/2016/11/201611151432113211.pdf
[8]	国家卫生健康委办公厅, 科技教育司办公室. 新型冠状病毒实验室生物安全指南（第二版）[OB/OL]. （2020-01-23）. http://news.bioon.com/article/6749573.html
[9]	国家卫生健康委办公厅, 国家科技教育司办. 关于印发新型冠状病毒实验室生物安全指南（第二版）的通[OB/OL]. （2020-01-23 ）. https://news.sciencenet.cn/htmlnews/2020/1/435132.shtm
[10]	Bogoch I I,Watts A,Thomas-Bachli A,et al. Potential for global spread of a novel coronavirus from China[J]. J Travel Med,2020,27(2):taaa011. doi: 10.1093/jtm/taaa011
[11]	Lu R,Zhao X,Li J,et al. Genomic characterisation and epidemiology of 2019 novel coronavirus:Implications for virus origins and receptor binding[J]. Lancet,2020,395(10224):565-574. doi: 10.1016/S0140-6736(20)30251-8
[12]	Su S,Wong G,Shi W,et al. Epidemiology,genetic recombination,and pathogenesis of coronaviruses[J]. Trends Microbiol,2016,24(6):490-502. doi: 10.1016/j.tim.2016.03.003
[13]	Zhu N,Zhang D,Wang W,et al. A novel coronavirus from patients with pneumonia in China,2019[J]. N Engl J Med,2020,382(8):727-733. doi: 10.1056/NEJMoa2001017
[14]	施绍瑞,聂滨,郭渝,等. 新型冠状病毒肺炎病例多种生物样本的病毒核酸检测结果[J]. 华西医学,2020,35(2):132‐136.
[15]	刘焱斌,刘涛,崔跃,等. 鼻拭子与咽拭子两种取样方法在新型冠状病毒肺炎核酸筛检中的比较研究[J]. 中国呼吸与危重监护杂志,2020,19(2):141-143.
[16]	Zou L R,Ruan F,Huang M X,et al. SARS-CoV-2 viral load in upper respiratory specimens of infected patients[J]. N Engl J Med,2020,382(12):1177-1179. doi: 10.1056/NEJMc2001737
[17]	XU Z,SHI L,WANG Y J,et al. Pathological findings of COVID-19 associated with acute respiratory distress syndrome[J]. Lancet Respir Med,2020,8(4):420-422. doi: 10.1016/S2213-2600(20)30076-X
[18]	Zhang H, Kang Z, Gong H, et al. The digestive system is a potential route of 2019- nCov infection: a bioinformatics analysis based on single-cell transcriptomes [J/OL]. https://doi. org/10.1101/ 2020.01.30.927806.
[19]	俞龙,彭伟,朱丹. 2019 冠状病毒病患者出院前核酸检测粪便标本阳性而肛拭、咽拭子阴性1例报告[J]. 解放军医学院学报,2020,41(2):125-126. doi: 10.3969/j.issn.2095-5227.2020.02.008
[20]	吴建国,罗建飞,刘家盛,等. 重型/危重型新型冠状病毒肺炎患者多种生物样本核酸检测结果分析[J]. 解放军医学院学报,2020,41(3):205-207,211. doi: 10.3969/j.issn.2095-5227.2020.03.001
[21]	魏秋华,任哲. 2019新型冠状病毒感染的肺炎疫源地消毒措施[J]. 中国消毒学杂志,2020,37(1):59-62. doi: 10.11726/j.issn.1001-7658.2020.01.019

Relative Articles(20)

Relative Articles

[1]	Songqin LYU, Shan HUANG, Litang MA, Xiu WANG, Hongtao ZHANG, Qin LI, Chunping WAN, Zhengchao LYU. The Investigation and Analysis of the Situation of COVID-19 Vaccination and Vaccination Willingness in HIV/AIDS Population in Yunnan Province. Journal of Kunming Medical University, doi: 10.12259/j.issn.2095-610X.S20240108
[2]	Haiping HE. Analysis of Coagulation Function Indexes in Elderly Patients with Novel Coronavirus Pneumonia. Journal of Kunming Medical University, doi: 10.12259/j.issn.2095-610X.S20240414
[3]	Ying HUANG, Yun LUO, Yan WU, Bing LIU, Shan JIANG, Hua HE. The Regularity and Clinical Manifestations of Throat Swab and Fecal Detoxification in New Coronavirus Infected Patients. Journal of Kunming Medical University, doi: 10.12259/j.issn.2095-610X.S20220112
[4]	Hanqing ZHAO, Shibing QIAN, Mengqi WANG, Rongyu YANG, Kaiwen DUAN. Oral Lesions Associated with 2019-nCoV. Journal of Kunming Medical University, doi: 10.12259/j.issn.2095-610X.S20220831
[5]	Juan ZHAO, Teng-can TU, Mei-jun YANG, Xiao-yong ZHANG, Wei PAN. Assessment of Early Coronary Artery Disease in Patients with Acute Coronary Syndrome. Journal of Kunming Medical University, doi: 10.12259/j.issn.2095-610X.S20211219
[6]	Li WU, Yun-hui YANG, Jing ZHANG, Zhang-cong YANG, Yu SU, Yong-jian WEN, Yu-chun WU, Peng WANG, Zhi-peng LI, Wei ZHAO, Dan HAN, Rui-dong WANG, Bo HE. The Correlation between the Imaging Characteristics of Imported COVID-19 at First Diagnosis and Clinical Severity Grading. Journal of Kunming Medical University, doi: 10.12259/j.issn.2095-610X.S20210708
[7]	Yun-hui YANG, Bo HE, Ding-yun YOU, Jun-tao YANG, Qian GAO. Applications of CT in the Early Prediction of Different Clinical Types of COVID -19 Pneumonia. Journal of Kunming Medical University, doi: 10.12259/j.issn.2095-610X.S20210524
[8]	Huan LI, Qiu-yue XU, Yun-juan WANG, Yang SU, Rui-zhu TANG. The Rapid Detection of Nucleic Acid in SARS-CoV-2 by Reverse Transcription Loop Mediated Isothermal Amplification. Journal of Kunming Medical University, doi: 10.12259/j.issn.2095-610X.S20210904
[9]	Yin Li Juan , Bao Ling Yun , Li Ling , Yuan Yuan . . Journal of Kunming Medical University,
[10]	Li-fen ZHANG, Xue-hua ZHOU, Min XU, Li-li JIANG, Jian-ping CUN, Xiao-fang ZHOU, Xiao-qing FU, Bing-jun TIAN. Genetic Characteristic Analysis of Coxsackievirus A16 in Wenshan Prefecture of Yunnan Province in 2019. Journal of Kunming Medical University, doi: 10.12259/j.issn.2095-610X.S20201128
[11]	Ma Zhi Qiang , Wang Lin , Luo Yu , Qu Chun Yan , Li Bao Ping , Ma Yun Shan , Dong Rui , Wu Yan Rong , Zhao Jin Hua , Xu Ya Lin . . Journal of Kunming Medical University,
[12]	Yuan Yu , Li Xiang . . Journal of Kunming Medical University,
[13]	Ye Wen Jun , Wang Rui Hang , Zeng Xiao Ju , Liu Wei Qing , Liu Fang . . Journal of Kunming Medical University,
[14]	Gu Li Ming , Zeng Yong , Chen Jun Hui , Wang Fu Ping , Hao Ying Lu , Wu Hai Ying , Ruan Ji Yin , Li Yan Jiao , Chen Yun , Xia Jing , Sun Jia , Hu Juan Yuan , Li Wei , Yu Cong Tao , Ma Chao Xia , Qian Chuan Yun , Hu Min . . Journal of Kunming Medical University,
[15]	Tang Rui Zhu , Luo Zheng Qiong , Xu Qiu Yue , Zhao Ying , Li Huan , Wang Yun Juan , Song Yu , Liu Zi Jie . The Application of Loop-mediated Amplification Chip in Gene Testing of Common Lower Respiratory Pathogens. Journal of Kunming Medical University,
[16]	Luo Yang . Curative Effect of Tanreqing Injection Combined with Ganciclovir in the Treatment of Pediatric Respiratory Syncytial Virus Pneumonia. Journal of Kunming Medical University,
[17]	Ren Xiao Li . . Journal of Kunming Medical University,
[18]	He Ying Hong . . Journal of Kunming Medical University,
[19]	. . Journal of Kunming Medical University,
[20]	. Clinical Results of 232 Treatment-naive HIV/AIDS Patients after HAART. Journal of Kunming Medical University,