Volume 44 Issue 7
Jul.  2023
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Song Tao , Xiao Fan . Influence of Tracheal Intubation Guided with Light Wand on Hemodynamics in Patients with Obstructive Sleep Apnea Syndrome[J]. Journal of Kunming Medical University, 2016, 37(12): 85-88.
Citation: Li ZHANG, Xi ZHAO, Huaxiang ZHAO, Bao XIONG, Jie XU, Guangping TANG, Zhuo YU, Peng CHEN. Effects of Euonymine on Proliferation，Migration，and Cell Cycle of HUVECs and VSMCs[J]. Journal of Kunming Medical University, 2023, 44(7): 1-8. doi: 10.12259/j.issn.2095-610X.S20230723
shu

Effects of Euonymine on Proliferation，Migration，and Cell Cycle of HUVECs and VSMCs

doi: 10.12259/j.issn.2095-610X.S20230723
  • 1.

    School of Pharmaceutical Sciences and Yunnan Key Laboratory of Pharmacology for Natural Products，Kunming Medical University，Kunming Yunan 650500

  • 2.

    Dept. of Cardiology，Ganmei Hospital of Kunming 1st People’s Hospital，Kunming Yunnan 650224

  • 3.

    Dept. of Cardiology，Wenshan People’s Hospital，Wenshan Yunnan 663007

  • 4.

    Dept. of Cardiology，The 1st Affiliated Hospital of Kunming Medical University，Kunming Yunnan 650032，China

  • Received Date: 2023-04-17
    Available Online: 2023-07-17
  • Publish Date: 2023-07-25
    Abstract
  •   Objective  To investigate the effects of Euonymine on isolated cultured human umbilical vein endothelial cells （HUVECs） and human vascular smooth muscle cells （VSMCs）, so as to elucidate the possible mechanism of Euonymine in prevention and treatment of ISR.   Methods  The effect of trypan blue assay on the viability of HUVECs. MTT assay was used to observe the effect of Euonymine on the proliferation of normal HUVECs and VSMCs. A wound healing assay was performed to detect the effect of Euonymine on the migration of VSMCs.   Results  The MTT results showed that the absorbance of HUVECs and VSMCs decreased after the Euonymine intervention. In addition, the IC50 of Euonymine on HUVECs after 24 hours and on VSMCs after 48 hours were 28.6 µg/mL and 33.92 µg/mL, respectively. Flow cytometry results showed that Euonymine （25 μg/mL） arrested the cell cycle of both HUVECs and VSMCs at the G0/G1 phase, while Euonymine （100 μg/mL） blocked the mitosis of VSMCs at both the G0/G1 and G2/M phases.   Conclusion  Euonymine affects the cell cycle mainly by inhibiting the proliferation and migration of VSMCs and has potential value of clinical applications in inhibiting neointima hyperplasia after percutaneous coronary intervention （PCI）.
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