Effects of Ferroptosis Inhibitor KIF20A on Biological Behavior and Ferroptosis of Esophageal Carcinoma Cells

Maimaitizunong Rezeye; Xiujuan LI; Ling LIU; Hui LI

doi:10.12259/j.issn.2095-610X.S20240207

Volume 45 Issue 2

Feb. 2024

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Maimaitizunong Rezeye, Xiujuan LI, Ling LIU, Hui LI. Effects of Ferroptosis Inhibitor KIF20A on Biological Behavior and Ferroptosis of Esophageal Carcinoma Cells[J]. Journal of Kunming Medical University, 2024, 45(2): 49-56. doi: 10.12259/j.issn.2095-610X.S20240207

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Effects of Ferroptosis Inhibitor KIF20A on Biological Behavior and Ferroptosis of Esophageal Carcinoma Cells

doi: 10.12259/j.issn.2095-610X.S20240207

1.
Dept. of Biochemistry and Molecular Biology，School of Basic Medicine
2.
Dept. of Pathophysiology
3.
Central Laboratory，Xinjiang Medical University，Urumqi Xinjiang 830000，China

Received Date: 2023-11-09
Available Online: 2024-02-27
Publish Date: 2024-02-25

Abstract

Abstract

Objective To investigate the expression of KIF20A in ESCC and its effect on Eca109 cell biological behavior and ferroptosis. Methods The expression of KIF20A in ESCC was predicted by bioinformatics analysis. KIF20A knockdown /overexpression Eca109 cell line was constructed, Experimental groups: control group, KIF20A knockdown group, KIF20A overexpression group, and the effects of KIF20A on the proliferation, invasion, and migration of Eca109 cells were detected by CCK-8, Transwell invasion assay and cell scratch assay. On this basis, the ferroptosis model induced by RSL3 was established, and GSH and MDA contents were detected. Results Compared with the control group, the cell proliferation activity of the KIF20A knockdown group was significantly decreased （P < 0.05）, and that of the KIF20A overexpression group was significantly increased （P < 0.05）. Compared with the control group, the invasion and migration ability of the KIF20A knockdown group was decreased （P < 0.05）, and the invasion and migration ability of the KIF20A overexpression group was increased （P < 0.05）. After RSL3 induction, the GSH content in the cell lysate of the KIF20A knockdown group was lower than that of the control group （P < 0.05）, and the MDA content was higher than that of the control group （P < 0.05）. Conclusion KIF20A is highly expressed in ESCC and plays a role in promoting proliferation, invasion, migration and inhibiting ferroptosis in Eca109 cells.
- Ferroptosis,
- KIF20A,
- ESCC,
- RSL3

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References(16)

References

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