Analysis of Serum Biomarkers in Patients with Pulmonary Tuberculosis Complicated by Invasive Pulmonary Aspergillosis

Xiaoyan WANG; Xinrui WAN; Shitao XU; Wei CHEN; Hongyan MAO; Lin WANG; Zhiqiang MA

doi:10.12259/j.issn.2095-610X.S20250320

Volume 46 Issue 3

Apr. 2025

Turn off MathJax

Article Contents

Article Navigation > Journal of Kunming Medical University > 2025 > 46(3): 132-138

Xiaoyan WANG, Xinrui WAN, Shitao XU, Wei CHEN, Hongyan MAO, Lin WANG, Zhiqiang MA. Analysis of Serum Biomarkers in Patients with Pulmonary Tuberculosis Complicated by Invasive Pulmonary Aspergillosis[J]. Journal of Kunming Medical University, 2025, 46(3): 132-138. doi: 10.12259/j.issn.2095-610X.S20250320

Citation:

Xiaoyan WANG, Xinrui WAN, Shitao XU, Wei CHEN, Hongyan MAO, Lin WANG, Zhiqiang MA. Analysis of Serum Biomarkers in Patients with Pulmonary Tuberculosis Complicated by Invasive Pulmonary Aspergillosis[J]. Journal of Kunming Medical University, 2025, 46(3): 132-138. doi: 10.12259/j.issn.2095-610X.S20250320

Citation:

Xiaoyan WANG, Xinrui WAN, Shitao XU, Wei CHEN, Hongyan MAO, Lin WANG, Zhiqiang MA. Analysis of Serum Biomarkers in Patients with Pulmonary Tuberculosis Complicated by Invasive Pulmonary Aspergillosis[J]. Journal of Kunming Medical University, 2025, 46(3): 132-138. doi: 10.12259/j.issn.2095-610X.S20250320

PDF( 1114 KB)

Analysis of Serum Biomarkers in Patients with Pulmonary Tuberculosis Complicated by Invasive Pulmonary Aspergillosis

doi: 10.12259/j.issn.2095-610X.S20250320

Department of Laboratory Medicine，The Third People’s Hospital of Kunming/Clinical Medical Center for Infectious Diseases of Yunnan Province，Kunming Yunnan 650301，China

Received Date: 2024-09-09
Available Online: 2025-02-24
Publish Date: 2025-04-09

Abstract

Abstract

Objective To analyze the diagnostic value of serum biomarkers in patients with pulmonary tuberculosis complicated by invasive pulmonary aspergillosis. Methods A retrospective collection of laboratory test results, including blood analysis, liver function, lymphocyte counts, and cytokine levels, from 54 patients diagnosed with pulmonary tuberculosis and invasive pulmonary aspergillosis admitted to the Third People's Hospital of Kunming between January 2021 and May 2024. Additionally, 70 patients with simple pulmonary tuberculosis and 50 healthy individuals were collected as control groups to compare serum biomarker levels across the three groups and analyze relevant factors and diagnostic value for pulmonary tuberculosis patients with invasive pulmonary aspergillosis. Results Among different age groups, the incidence of pulmonary tuberculosis with invasive pulmonary aspergillosis was 29 cases （53.7%） in youth, 15 cases （27.8%） in middle age, and 10 cases （18.5%） in the elderly. In terms of gender distribution, there were 41 males （75.9%） and 13 females （24.1%）. The serum levels of CRP （6.85 [2.10, 27.0]） ng/L, PCT （0.05 [0.05, 0.15]） ng/mL, RBC （4.55±0.65）×10¹²/L, Hb （129.13±19.10） g/L, TP （66.23±6.82） g/L, ALB （37.03±4.77） g/L, and CHOL （4.30 [3.71, 4.91]） mmol/L in the invasive pulmonary aspergillosis group showed no significant difference compared to the simple tuberculosis group and healthy control group （P > 0.05）. The levels of CD3+ T, CD4+ T, and CD8+ T in the invasive pulmonary aspergillosis group were significantly lower than those in the simple tuberculosis group and healthy control group （P < 0.05）. The levels of IL-2, IL-4, IL-5, IL-8, IL-10, IL-12p70, IFN - α, and TNF - α in the invasive pulmonary aspergillosis group were significantly higher than those in the healthy control group （P < 0.05）; IL-8, IL-12p70, and IFN-α were also higher compared to the simple tuberculosis group, with statistical significance （P < 0.05）. Conclusion The population with pulmonary tuberculosis complicated by invasive pulmonary aspergillosis is predominantly male and younger.The serum indicators of infection severity and nutritional status in these patients are similar to those with simple tuberculosis and lack specificity; however, their immune function is significantly lower than that of simple tuberculosis patients. Multiple cytokines are elevated, particularly IL-8, IL-12p70, and IFN-α, which can aid in the differential diagnosis of pulmonary tuberculosis infection.
- Pulmonary tuberculosis,
- Invasive pulmonary aspergillosis,
- Serum biomarkers,
- Diagnostic value

FullText(HTML)

References(17)

References

[1]	Richterman A,Steer-Massaro J,Jarolimova J,et al. Cash interventions to improve clinical outcomes for pulmonary tuberculosis: Systematic review and meta-analysis[J]. Bull World HealthOrganization,2018,96(7):471-483. doi: 10.2471/BLT.18.208959
[2]	徐艺铭,刘慧. 侵袭性肺曲菌病和浸润型肺结核的影像鉴别[J]. 影像研究与医学应用,2021,5(20):39-41. doi: 10.3969/j.issn.2096-3807.2021.20.017
[3]	孟原竹,蒋国路,陈小兵,等. 肺结核合并侵袭性肺曲霉感染临床特征及危险因素分析[J]. 中华肺部疾病杂志(电子版),2023,16(4):541-543.
[4]	林俊杰,李嘉琪,罗素云,等. 胸部CT 结合床旁气管镜对侵袭性肺曲霉病的早期诊断价值研究[J]. 中国CT和MRI杂志,2023,21(4):73-75. doi: 10.3969/j.issn.1672-5131.2023.04.026
[5]	张小红,周宸,罗远明,等. 慢性阻塞性肺疾病急性加重患者合并侵袭性肺曲霉菌病的临床特征及相关因素分析[J]. 中华医学杂志,2023,103(22):1692-1699. doi: 10.3760/cma.j.cn112137-20221106-02333
[6]	中华人民共和国国家卫生和计划生育委员会. 肺结核诊断标准(WS 288-2017)[J]. 中国感染控制杂志,2018,17(7):642-652.
[7]	De Pauw B,Walsh T J,Donnelly J P,et al. Revised definitions of invasive fungal disease from the European Organizationfor Research and Treatment of Cancer/Invasive Fungal InfectionsCooperative Group and the National Institute of Allergy and Infectious Diseases Mycoses Study Group (EORTC/MSG) ConsensusGroup[J]. Clin Infect Dis,2008,16(12):1813-1821.
[8]	中华医学会呼吸病学分会感染学组,中华结核和呼吸杂志编辑委员会. 肺真菌病诊断和治疗专家共识[J]. 中华结核和呼吸杂志,2007,30(11):821-834. doi: 10.3760/j.issn:1001-0939.2007.11.008
[9]	Bagcchi Sanjeet. WHO’ s global tuberculosis report 2022[J]. The Lancet Microbe,2023,4(1):e20.
[10]	舒薇,刘宇红. 世界卫生组织《2023年全球结核病报告》解读[J]. 结核与肺部疾病杂志,2024,5(1):15-19.
[11]	邢二庆,王成祥,尚家星,等. 儿童肾病综合征并发侵袭性真菌感染危险因素及血清 PCT 和 CRP 水平[J]. 中华医院感染学杂志,2021,31(13):1987-1991.
[12]	刘锴. 急性肺曲霉菌病动物模型建立及肿块型肺真菌病CT影像学特征研究[D]. 上海:第二军医大学,2011.
[13]	Shi C,Shan Q,Xia J,et al. Incidence,risk factors and mortality of invasive pulmonary aspergillosis in patients with influenza: A systematic review and meta-analysis[J]. Mycoses,2022,65(2):152-163.
[14]	Yerbnaga I W,Nakanabo Diallo S,Rouamba T,et al. A systematic review of epidemiology,risk factors,diagnosis,antifungal resistance,and management of invasive aspergillosis inAfrica[J]. J Mycol Med,2022,33(1):101328.
[15]	林亚纳,张小菲,洪亚妮,等. 不同免疫状态侵袭性肺曲霉菌病患者实验室指标分析[J]. 中国免疫学杂志,2023,39(10):2211-2216. doi: 10.3969/j.issn.1000-484X.2023.10.034
[16]	胡传玺,刘灵燕,李漫. 血清白细胞介素-10、白细胞介素-17、白细胞介素-23检测对慢性阻塞性肺疾病合并侵袭性肺曲霉菌感染的诊断及预后评估价值[J]. 陕西医学杂志,2023,52(10):1415-1418. doi: 10.3969/j.issn.1000-7377.2023.10.030
[17]	邵长周,瞿介明,何礼贤,等. 侵袭性肺曲霉菌病鼠模型的建立及γ-干扰素和肺组织细菌负荷的动态变化[J]. 中国实用内科杂志,2007,(21):1725-1728. doi: 10.3969/j.issn.1005-2194.2007.21.029

Relative Articles(20)

Relative Articles

[1]	Furong ZHANG, Yidan YU, Jiarui ZHANG, Xiujun LUO, Xinyue LI, Qi DENG, Zhong SUN, Guozhong HE. Epidemiological Characteristics and Spatial Distribution of Pulmonary Tuberculosis in Lanping County from 2018 to 2023. Journal of Kunming Medical University, 2025, 46(6): 20-28. doi: 10.12259/j.issn.2095-610X.S20250603
[2]	Lingjun SHEN, Longfen LI, Chunjing SHI, Wenming LI, Yuanqing HUANG, Huajie ZHANG, Yun LUO, Jie LI, Li LIU. Factors Influencing the Development of Extra-Pulmonary Tuberculosis in Patients with Viral Hepatitis Complicated by Pulmonary Tuberculosis. Journal of Kunming Medical University, 2025, 46(2): 103-109. doi: 10.12259/j.issn.2095-610X.S20250215
[3]	Chunjing SHI, Wenming LI, Yun LUO, Huajie ZHANG, Liuxin HAN, Yanhong ZHAO, Longfen LI, Lingjun SHEN. The Value of Fitting NRS 2002 ，CONUT ，RDW-SD and ALB in Assessing The Risk of Venous Thromboembolism in Patients with the Pulmonary Tuberculosis Based on Lasso-Logistic Regression Model. Journal of Kunming Medical University, 2025, 46(8): 1-10.
[4]	Jihong LIU, Zhiqiang MA, Lin WANG, Xiaoyan WANG, Weiguang TIAN, Baoping LI, Rui DONG, Yujia LV. Correlation Analysis of Immune Function of Tuberculosis Patients Infected with COVID-19. Journal of Kunming Medical University, 2024, 45(4): 157-162. doi: 10.12259/j.issn.2095-610X.S20240423
[5]	Liqiang LAN, Jing LI, Qingbo REN. Value Evaluation of New Markers AFP-L3 and PIVKAⅡ in the Diagnosis of Liver Cancer. Journal of Kunming Medical University, 2023, 44(7): 136-141. doi: 10.12259/j.issn.2095-610X.S20230705
[6]	Xin WU, Pin-fei ZOU, Min HUANG. Diagnostic Value of Guidewire Localization of Breast Mass Based on High-frequency Ultrasound Guidance in Breast Lesions. Journal of Kunming Medical University, 2022, 43(2): 67-71. doi: 10.12259/j.issn.2095-610X.S20220216
[7]	Na LI, Jie CHEN, Ji LUO, Yong-gang CHEN, Jiang-li PENG, Ming-li YU, Sheng-hao LI. Retrospective Analysis of 376 Cases of Adverse Reactions to Antituberculosis Drug. Journal of Kunming Medical University, 2021, 42(4): 117-121. doi: 10.12259/j.issn.2095-610X.S20210422
[8]	Yan-ping LI, Yi ZENG, Xiao-lin DONG, Huan WEI, Qing-yun LI, Ming-shun AN, Li-ping ZHAN, Jian-hui LI, Gang WU, Yang LI, Jing-hui LI. Related Factors Affecting Adverse Events of Symptomatic Carotid Artery Stenosis. Journal of Kunming Medical University, 2021, 42(7): 104-109. doi: 10.12259/j.issn.2095-610X.S20210717
[9]	Zhe GE, Si-ping LU, Shi-zhong FU, Chun-hong WANG, Shao-ying LI, Ao LIU. The Preliminary Application of Whole Blood Interferon-Gamma Release Assay Combined with T Lymphocyte Subset Detection in Diagnosis of Pulmonary Tuberculosis. Journal of Kunming Medical University, 2021, 42(9): 108-113. doi: 10.12259/j.issn.2095-610X.S20210901
[10]	Xing LIU, Bing OUYANG, Na LI, Ji LUO, Xu-qin SHI, Jie CHEN, Hui SUN, Hai-wen LI. Effect and Economic Evaluation of the Combination of Traditional Chinese and Western Medicine in Treatment of MDR-TB. Journal of Kunming Medical University, 2020, 41(11): 49-54. doi: 10.12259/j.issn.2095-610X.S20201106
[11]	Rong WAN, Ming-wu LI, Meng MA, Ming-hong LAI, Guang-mei Li. Efficacy of T-SPOT.TB in Treatment of Active Tuberculosis. Journal of Kunming Medical University, 2020, 41(12): 133-137. doi: 10.12259/j.issn.2095-610X.S20201237
[12]	Ran Hai Liang , Yang Qing Huan , Chen Ying , Xiong Li Fen , Deng Yi Yu , He Xiu Yun . . Journal of Kunming Medical University, 2020, 41(06): 46-50.
[13]	Jie Chun Lin , Huang Tao , Teng Zhao Wei , Bu Jun Hui , Li Meng Zhou , Zhang Jing. . Journal of Kunming Medical University, 2019, 40(04): 91-96.
[14]	Hong Xu Hua . Effects of Tuberculosis Drugs Combined with Rifapentine on the Immune Function， Inflammatory Factors， VEGF and MMP-9 of TB Patients. Journal of Kunming Medical University,
[15]	Jia Liang . The Diagnostic Value of Plasma Fibrinogen and D-dimer for the Progressive Stroke. Journal of Kunming Medical University,
[16]	Lei Yu . . Journal of Kunming Medical University,
[17]	Lu Ni Hong . . Journal of Kunming Medical University,
[18]	Lu Ni Hong . . Journal of Kunming Medical University,
[19]	Lu Ni Hong . Clinical Characteristics of Pulmonary Tuberculosis Patients with and without HIV(+)/AIDS. Journal of Kunming Medical University,
[20]	. The Value of T Cells Spots Test in the Diagnosis of Human Immunodeficiency Virus Amalgamative Tuberculosis Pateints with Different Levels of T cells. Journal of Kunming Medical University,