Tanshinone Ⅰ Alleviates Sepsis Associated Acute Kidney Injury in Rats by Regulating Wnt/β-catenin Signaling Pathway

Jingyu REN; Xingpeng JIANG; Zhengchao LI; Shiyuan WEN; Sha ZHU; Jin RU

doi:10.12259/j.issn.2095-610X.S20250604

Volume 46 Issue 6

Jun. 2025

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Jingyu REN, Xingpeng JIANG, Zhengchao LI, Shiyuan WEN, Sha ZHU, Jin RU. Tanshinone Ⅰ Alleviates Sepsis Associated Acute Kidney Injury in Rats by Regulating Wnt/β-catenin Signaling Pathway[J]. Journal of Kunming Medical University, 2025, 46(6): 29-37. doi: 10.12259/j.issn.2095-610X.S20250604

Citation:

Jingyu REN, Xingpeng JIANG, Zhengchao LI, Shiyuan WEN, Sha ZHU, Jin RU. Tanshinone Ⅰ Alleviates Sepsis Associated Acute Kidney Injury in Rats by Regulating Wnt/β-catenin Signaling Pathway[J]. Journal of Kunming Medical University, 2025, 46(6): 29-37. doi: 10.12259/j.issn.2095-610X.S20250604

Citation:

Jingyu REN, Xingpeng JIANG, Zhengchao LI, Shiyuan WEN, Sha ZHU, Jin RU. Tanshinone Ⅰ Alleviates Sepsis Associated Acute Kidney Injury in Rats by Regulating Wnt/β-catenin Signaling Pathway[J]. Journal of Kunming Medical University, 2025, 46(6): 29-37. doi: 10.12259/j.issn.2095-610X.S20250604

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Tanshinone Ⅰ Alleviates Sepsis Associated Acute Kidney Injury in Rats by Regulating Wnt/β-catenin Signaling Pathway

doi: 10.12259/j.issn.2095-610X.S20250604

1.
Department of Intensive Care Unit，The First People’s Hospital of Yunnan Province，Kunming Yunnan 650032
2.
Graduate School，Kunming University of Science and Technology，Kunming Yunnan 650093，China

Received Date: 2024-07-25
Publish Date: 2025-06-25

Abstract

Abstract

Objective To investigate the effect of Tan Ⅰ on SA-AKI in rats by mediating Wnt/β-catenin signaling pathway. Methods Sprague-Dawley rats were randomly divided into the following groups （n = 8 per group, including 2 reserve animals per group）: Sham, SA-AKI, SA-AKI + 5 mg/kg Tan I, SA-AKI + 10 mg/kg Tan I, SA-AKI + 15 mg/kg Tan I （SA-AKI+Tan I）, SA-AKI + salinomycin sodium （SS, Wnt signal inhibitor, SA-AKI + SS）, SA-AKI + SS + Tan I, SA-AKI + laduviglusib （LG, Wnt signal activator, SA-AKI + LG）, and SA-AKI + LG + Tan I. Rat SA-AKI model was induced by cecal ligation and puncture （CLP）, with Tan I, SS, and LG administered via intraperitoneal injection. Hematoxylin-eosin and TUNEL staining were used to observe renal tissue pathological damage. Enzyme-linked immunosorbent assay was used to detect serum concentrations of neutrophil gelatinase-associated lipocalin （NGAL）, IL-1β, IL-8, IL-6, and TNF-α. Creatinine （Cre） and blood urea nitrogen （BUN） kit were used to detect serum Cre and BUN concentrations. Western blot and immunofluorescence staining were used to detect the expression and fluorescence intensity of Wnt1, GSK3β, and β-catenin. Results Administration of Tan I at doses of 10 mg/kg and 15 mg/kg significantly attenuated renal injury in rats with SA-AKI （P < 0.05）, suppressed the levels of SA-AKI biomarkers NGAL, Cre, and BUN and pro-inflammatory cytokines （P < 0.05）, reduced apoptosis, and downregulated Wnt1 and GSK3β while upregulating β-catenin expression （P < 0.05）. Although Tan I at 5 mg/kg exhibited a modest protective effect against SA-AKI in rats, no statistically significant difference was observed compared to the sham group （P > 0.05）. SS weakened CLP-induced kidney injury and the production of inflammatory cytokines in rats （P < 0.05）, and LG further aggravated CLP-induced kidney injury in rats （P < 0.05）. Tan Ⅰ reversed the promoting effect of LG on kidney injury in SA-AKI rats （P < 0.05）. Conclusion Tan Ⅰ provides a protective effect on CLP-induced SA-AKI rat by inhibiting Wnt/β-catenin signaling pathway.
- Sepsis associated acute kidney injury,
- Tanshinone Ⅰ,
- Wnt signaling pathway,
- Kidney function,
- Inflammatory cytokines

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References(28)

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