EIF5A1 Promotes Proliferation，Migration and Invasion of Intrahepatic Cholangiocarcinoma Cells Through Wnt Signaling Pathway

Shaohua YANG; Yongping XU; Zhuoyu ZHAO; Xingbao FANG; Zhenxing RUAN

doi:10.12259/j.issn.2095-610X.S20250706

Volume 46 Issue 7

Jul. 2025

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Shaohua YANG, Yongping XU, Zhuoyu ZHAO, Xingbao FANG, Zhenxing RUAN. EIF5A1 Promotes Proliferation，Migration and Invasion of Intrahepatic Cholangiocarcinoma Cells Through Wnt Signaling Pathway[J]. Journal of Kunming Medical University, 2025, 46(7): 46-53. doi: 10.12259/j.issn.2095-610X.S20250706

Citation:

Shaohua YANG, Yongping XU, Zhuoyu ZHAO, Xingbao FANG, Zhenxing RUAN. EIF5A1 Promotes Proliferation，Migration and Invasion of Intrahepatic Cholangiocarcinoma Cells Through Wnt Signaling Pathway[J]. Journal of Kunming Medical University, 2025, 46(7): 46-53. doi: 10.12259/j.issn.2095-610X.S20250706

Citation:

Shaohua YANG, Yongping XU, Zhuoyu ZHAO, Xingbao FANG, Zhenxing RUAN. EIF5A1 Promotes Proliferation，Migration and Invasion of Intrahepatic Cholangiocarcinoma Cells Through Wnt Signaling Pathway[J]. Journal of Kunming Medical University, 2025, 46(7): 46-53. doi: 10.12259/j.issn.2095-610X.S20250706

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EIF5A1 Promotes Proliferation，Migration and Invasion of Intrahepatic Cholangiocarcinoma Cells Through Wnt Signaling Pathway

doi: 10.12259/j.issn.2095-610X.S20250706

1.
Department of Hepatobiliary Pancreatic Surgery，First People's Hospital of Qujing City，Qujiing Yunnan 655000
2.
Department of Hepatobiliary Surgery，Fourth People's Hospital of Changzhou City，Changzhou Jiangsu 213000，China

Received Date: 2025-03-23
Publish Date: 2025-07-21

Abstract

Abstract

Objective To investigate the expression of EIF5A1 in intrahepatic cholangiocarcinoma cell lines and human hepatobiliary duct epithelia, and its effect on the proliferation, migration and invasion ability and Wnt/β-Catenin signaling pathway in HUCCT1 cells . Methods Western blot was used to detect the basal expression level of EIF5A1 in intrahepatic cholangiocarcinoma cell lines and human intrahepatic cholangiocarcinoma epithelial cells. Transient transfection of siRNA was used to silence the expression of EIF5A1 in intrahepatic cholangiocarcinoma cell HUCCT1. The experimental groups were divided into blank control group (Con), siRNA1 group, and siRNA2 group. The most effective siRNA was screened by Western blot. The effects of EIF5A1 silencing on the proliferation, migration and invasion ability of HUCCT1 cells were detected by CCK-8, EdU cell proliferation assay and Transwell assay. The effect of EIF5A1 silencing on the Wnt/β-Catenin signaling pathway in HUCCT1 cells was detected by Western blot. Results The results of CCK-8 and EdU cell proliferation experiments showed that the proliferation ability of HUCCT1 cells decreased after EIF5A1 silencing （P < 0.05）, and Transwell migration and invasion experiments showed that the migration and invasion ability of Hucct1 cells decreased after EIF5A1 silencing （P < 0.05）. Western blot analysis revealed decreased expression of β-Catenin, Cyclin D1, MMP-2 and Survivin in Wnt/β-Catenin signaling pathway after EIF5A1 silencing （P < 0.05）. Conclusion EIF5A1 may promote the proliferation, migration and invasion of intrahepatic bile duct cancer cells through Wnt/β-Catenin signaling pathway.
- Intrahepatic cholangiocarcinoma,
- EIF5A1,
- Wnt/β-Catenin signaling pathway,
- Proliferation,
- Migration and invasion

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References(32)

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