Relationship of Serum IL-27，CysC，GAPDH Antibodies with Disease Severity and Prognosis in Children with Neuromyelitis Optica Spectrum Disorder

Zuohua WANG; Rongfeng WU; Xiaoyan ZHANG; Huiping WANG

doi:10.12259/j.issn.2095-610X.S20250718

Volume 46 Issue 7

Jul. 2025

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Article Navigation > Journal of Kunming Medical University > 2025 > 46(7): 146-154

Zuohua WANG, Rongfeng WU, Xiaoyan ZHANG, Huiping WANG. Relationship of Serum IL-27，CysC，GAPDH Antibodies with Disease Severity and Prognosis in Children with Neuromyelitis Optica Spectrum Disorder[J]. Journal of Kunming Medical University, 2025, 46(7): 146-154. doi: 10.12259/j.issn.2095-610X.S20250718

Citation:

Zuohua WANG, Rongfeng WU, Xiaoyan ZHANG, Huiping WANG. Relationship of Serum IL-27，CysC，GAPDH Antibodies with Disease Severity and Prognosis in Children with Neuromyelitis Optica Spectrum Disorder[J]. Journal of Kunming Medical University, 2025, 46(7): 146-154. doi: 10.12259/j.issn.2095-610X.S20250718

Citation:

Zuohua WANG, Rongfeng WU, Xiaoyan ZHANG, Huiping WANG. Relationship of Serum IL-27，CysC，GAPDH Antibodies with Disease Severity and Prognosis in Children with Neuromyelitis Optica Spectrum Disorder[J]. Journal of Kunming Medical University, 2025, 46(7): 146-154. doi: 10.12259/j.issn.2095-610X.S20250718

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Relationship of Serum IL-27，CysC，GAPDH Antibodies with Disease Severity and Prognosis in Children with Neuromyelitis Optica Spectrum Disorder

doi: 10.12259/j.issn.2095-610X.S20250718

1.
Department of Neurology
2.
Department of Cardiovascular Medicine，Kunming Children’s Hospital/Affiliated Children’s Hospital of Kunming Medical University，Kunming Yunnan 650228，China

Received Date: 2025-05-09
Publish Date: 2025-07-21

Abstract

Abstract

Objective To explore the relationship between serum Interleukin-27 （IL-27）, Cystatin C （CysC）, and Glyceraldehyde-3-phosphate dehydrogenase （GAPDH） antibodies and the severity of disease in children with Neuromyelitis optica spectrum disorder （NMOSD）, as well as their impact on prognosis. Methods A total of 102 children with NMOSD admitted to Kunming Children's Hospital from July 2019 to July 2023 were selected, along with 102 healthy during the same period. Serum levels of IL-27, CysC, and GAPDH antibodies were compared between children with NMOSD and healthy children. The levels of serum IL-27, CysC, and GAPDH antibodies were compared among children with varying disease severity. The correlations between serum IL-27, CysC, GAPDH antibodies and disease condition, cerebrospinal fluid markers were analyzed. And 102 children with NMOSD received individualized treatment and were followed up for 1 year. The prognosis was evaluated based on disease relapse, and patients were divided into recurrence group and non-recurrence group. The clinical data, serum IL-27, CysC, and GAPDH antibody levels were compared between the two groups. the impact of serum IL-27, CysC, and GAPDH antibodies on disease recurrence was analyzed, and the predictive value of serum IL-27, CysC, and GAPDH antibodies for disease recurrence was evaluated. Results Children with NMOSD had lower levels of serum IL-27 and CysC and higher levels of GAPDH antibodies than healthy children （P < 0.05）. Serum IL-27 and CysC levels were negatively correlated with Aquaporin 4 （AQP4）-IgG antibody positivity, the number of spinal cord-involved segments, Expanded disability status scale （EDSS） scores, cerebrospinal fluid protein content, and white blood cell count. In contrast GAPDH antibodies were positively correlated with these parameters （P < 0.05）. After 1-year follow-up, 2 cases were lost to follow-up, 21 cases relapsed, and 79 cases did not, which were included in the relapse group and non-relapse group, respectively. There were significant differences in the number of spinal cord-involved segments, EDSS scores, and cerebrospinal fluid protein content between the relapse group and non-relapse group （P < 0.05）. The levels of serum IL-27 and CysC were lower and the levels of GAPDH antibodies were higher in the relapse group than the non-relapse group （P < 0.05）. Serum IL-27, CysC, and GAPDH antibodies were significantly associated with disease relapse （P < 0.05）. The Area under the curve （AUC） values for predicting disease recurrence in children with NMOSD based on serum IL-27, CysC, and GAPDH antibodies were 0.748, 0.791, and 0.747, respectively, with optimal cutoff values of 38.77 pg/mL, 0.79 mg/L, and 55.81 pg/mL, respectively. The combined prediction of disease relapse using these three markers had an AUC of 0.900, which was superior to individual prediction values （Z = 2.215, 2.137, 2.220, P = 0.024, 0.033, 0.023）. Conclusion The levels of serum IL-27, CysC, and GAPDH antibodies are significantly correlated with the disease severity and prognosis in children with NMOSD, and can effectively predict the risk of disease recurrence. Combined detection provides more reliable predictive value.
- Neuromyelitis optica spectrum disorders,
- Interleukin-27,
- Cystatin C,
- Glyceraldehyde-3-phosphate dehydrogenase antibody,
- The severity of the disease,
- Prognosis,
- Correlation

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References(27)

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