miR-16-5p Promotes Inflammation and Apoptosis in Oxygen-Glucose Deprivation Microglia Model by Mediating GPR30 Expression

Zhiyong LI; Zhenggang CHEN; Jun PENG; Dazhong LIANG

doi:10.12259/j.issn.2095-610X.S20251003

Volume 46 Issue 10

Oct. 2025

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Zhiyong LI, Zhenggang CHEN, Jun PENG, Dazhong LIANG. miR-16-5p Promotes Inflammation and Apoptosis in Oxygen-Glucose Deprivation Microglia Model by Mediating GPR30 Expression[J]. Journal of Kunming Medical University, 2025, 46(10): 23-31. doi: 10.12259/j.issn.2095-610X.S20251003

Citation:

Zhiyong LI, Zhenggang CHEN, Jun PENG, Dazhong LIANG. miR-16-5p Promotes Inflammation and Apoptosis in Oxygen-Glucose Deprivation Microglia Model by Mediating GPR30 Expression[J]. Journal of Kunming Medical University, 2025, 46(10): 23-31. doi: 10.12259/j.issn.2095-610X.S20251003

Citation:

Zhiyong LI, Zhenggang CHEN, Jun PENG, Dazhong LIANG. miR-16-5p Promotes Inflammation and Apoptosis in Oxygen-Glucose Deprivation Microglia Model by Mediating GPR30 Expression[J]. Journal of Kunming Medical University, 2025, 46(10): 23-31. doi: 10.12259/j.issn.2095-610X.S20251003

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miR-16-5p Promotes Inflammation and Apoptosis in Oxygen-Glucose Deprivation Microglia Model by Mediating GPR30 Expression

doi: 10.12259/j.issn.2095-610X.S20251003

1.
Department of Medical Quality Management
2.
Department of Neurosurgery，The First Affiliated Hospital of Hainan Medical University，Haikou Hainan 570102
3.
Department of Neurosurgery，Haikou Affiliated Hospital of Xiangya School of Medicine，Central South University，Haikou Hainan 570208，China
4.
School of Physical Therapy，Sehan University，Mokpo 58447，Korea

Received Date: 2025-06-25
Available Online: 2025-09-25
Publish Date: 2025-10-28

Abstract

Abstract

Objective To explore the molecular mechanism of miR-16-5p promoting apoptosis and inflammatory response by targeting GPR30 expression in an in vitro ischemic stroke microglia model （BV-2）. Methods An ischemic stroke cell model was established by subjecting BV-2 cells to oxygen-glucose deprivation （OGD）. qRT-PCR was utilized to assess the levels of miR-16-5p and GPR30 mRNA in OGD cells. A miR-16-5p inhibitor was transfected into OGD cells to silence miR-16-5p expression, and alterations in inflammatory response and apoptosis were measured using ELISA kits and Annexin V-FITC/PI staining. Starbase was employed to predict interactions, and dual-luciferase reporter gene assays were conducted to confirm that miR-16-5p targets the 3'-untranslated region （UTR） sequence of GPR30. Changes in cellular inflammatory response and apoptosis were evaluated by knocking down miR-16-5p and/or GPR30 in OGD cells. Results miR-16-5p expression was significantly elevated （P < 0.01）, while GPR30 expression was notably decreased （P < 0.01） in OGD-induced cells. Knockdown of miR-16-5p reduced the expression levels of inflammatory factors and the cell apoptosis ratio （P < 0.01）. Inhibition of miR-16-5p expression led to an upregulation of GPR30 mRNA and protein levels （P < 0.01）. Simultaneous silencing of both miR-16-5p and GPR30 partially enhanced inflammatory factor expression levels and the cell apoptosis ratio compared to cells transfected solely with the miR-16-5p inhibitor （P < 0.05）. Conclusion In the microglia OGD model, miR-16-5p triggers inflammatory responses and enhances apoptosis by inhibiting GPR30 expression.
- Ischemic stroke,
- miR-16-5p,
- GPR30,
- Inflammatory response,
- Apoptosis

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References(27)

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