miR-147a Regulates Ferroptosis and Affects Invasion and Metastasis of Cervical Cancer Cells

Chunyu ZHANG; Jian LUO; Qi ZHOU

doi:10.12259/j.issn.2095-610X.S20251006

Volume 46 Issue 10

Oct. 2025

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Chunyu ZHANG, Jian LUO, Qi ZHOU. miR-147a Regulates Ferroptosis and Affects Invasion and Metastasis of Cervical Cancer Cells[J]. Journal of Kunming Medical University, 2025, 46(10): 53-60. doi: 10.12259/j.issn.2095-610X.S20251006

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Chunyu ZHANG, Jian LUO, Qi ZHOU. miR-147a Regulates Ferroptosis and Affects Invasion and Metastasis of Cervical Cancer Cells[J]. Journal of Kunming Medical University, 2025, 46(10): 53-60. doi: 10.12259/j.issn.2095-610X.S20251006

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miR-147a Regulates Ferroptosis and Affects Invasion and Metastasis of Cervical Cancer Cells

doi: 10.12259/j.issn.2095-610X.S20251006

Chunyu ZHANG¹,
Jian LUO²,
Qi ZHOU^{1
,
,}

1.
Fourth Department of Gynecological Surgery
2.
Cardiopulmonary Function Testing Department，Affiliated Tumor Hospital of Xinjiang Medical University，Urumqi Xinjiang 830011，China

Received Date: 2025-03-08
Publish Date: 2025-10-28

Abstract

Abstract

Objective To investigate the regulatory role of miR-147a in affecting cell proliferation and metastasis of cervical cancer Hela cells through ferroptosis. Methods Hela cells were cultured and passaged, and then a Hela single-cell suspension was prepared and transfected. After transfection, the cells were divided into control group, miR-147a mimics NC group, miR-147a mimics group, miR-147a inhibitor NC group, miR-147a inhibitor group, and miR-147a mimics+Ferrostatin-1 （1 μM） group. MTT assay was used to detect cell proliferation, Transwell assay to assess cell invasion capacity, scratch test to evaluate cell healing ability, flow cytometry to detect reactive oxygen species （ROS） expression, and assay kits to measure expression changes of ferrous ions （Fe²⁺）, ferric ions （Fe³⁺）, lactoperoxidase （LPO）, glutathione peroxidase （GSH-PX）, and glutathione （GSH）. Results Compared to the control group, the miR-147a mimics group showed reduced cell proliferation rate, cell invasion quantity, and cell migration distance （P < 0.05）; the miR-147a inhibitor group exhibited increased cell proliferation rate, cell invasion quantity, and cell migration distance （P < 0.05）. Compared to the control group, the miR-147a mimics group showed increased Fe²⁺, LPO, and ROS expression, with decreased Fe³⁺, GSH-PX, and GSH expression （P < 0.05）. The miR-147a inhibitor group showed decreased Fe²⁺ , LPO, and ROS expression, with increased Fe³⁺, GSH-PX, and GSH expression（P < 0.05）. The miR-147a mimics + Ferrostatin-1 group exhibited increased cell proliferation rate, cell invasion quantity, and cell migration distance compared to the miR-147a mimics group （P < 0.05）. Compared to the miR-147a mimics group, the miR-147a mimics + Ferrostatin-1 group showed decreased Fe²⁺, LPO, and ROS expression levels （P < 0.05）, with increased Fe³⁺, GSH-PX, and GSH expression levels （P < 0.05）. Conclusion miR-147a can promote ferroptosis and inhibit the proliferation and metastasis of cervical cancer cells.
- Cervical cancer,
- miR-147a,
- Ferroptosis,
- Metastasis

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