Clinical Application of NETs in Diagnosis and Monitoring of Acute Ischemic Stroke

Wendi YANG; Yaxin WANG; Jing QIAN; Limin YIN; Fengting MU; Xiaoli ZHAO

doi:10.12259/j.issn.2095-610X.S20251012

Volume 46 Issue 10

Oct. 2025

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Wendi YANG, Yaxin WANG, Jing QIAN, Limin YIN, Fengting MU, Xiaoli ZHAO. Clinical Application of NETs in Diagnosis and Monitoring of Acute Ischemic Stroke[J]. Journal of Kunming Medical University, 2025, 46(10): 97-105. doi: 10.12259/j.issn.2095-610X.S20251012

Citation:

Wendi YANG, Yaxin WANG, Jing QIAN, Limin YIN, Fengting MU, Xiaoli ZHAO. Clinical Application of NETs in Diagnosis and Monitoring of Acute Ischemic Stroke[J]. Journal of Kunming Medical University, 2025, 46(10): 97-105. doi: 10.12259/j.issn.2095-610X.S20251012

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Clinical Application of NETs in Diagnosis and Monitoring of Acute Ischemic Stroke

doi: 10.12259/j.issn.2095-610X.S20251012

1.
Department of Medical Laboratory
2.
Department of Science and Education，Ganmei Hospital Affiliated to Kunming Medical University/The First People’s Hospital of Kunming，Kunming Yunnan 650224，China

Received Date: 2025-08-31
Available Online: 2025-10-11
Publish Date: 2025-10-28

Abstract

Abstract

Objective To explore the value of neutrophil extracellular trapping nets （neutrophil extracellular traps, NETs） in the diagnosis and monitoring of acute ischemic stroke （acute ischemic stroke, AIS）. Methods From June 2023 to February 2024, 63 patients newly diagnosed with AIS at the Ganmei Hospital Affiliated with Kunming Medical University were selected as the experimental group, and 58 non-AIS individuals matched in gender, age, and other characteristics were selected as the control group. The NETs levels of patients in the experimental group were detected before and 7-10 days after treatment, and general clinical data and related laboratory results were statistically analyzed. Results The experimental group showed significantly higher levels of NEUT, LYMPH, MONO, EO, FDP, D-dimer, and NETs compared to the control group （P < 0.05）. The NETs levels before treatment were significantly higher than those at 7～10 days after treatment and in the control group （P < 0.05）. The NETs levels 7～10 days after treatment were significantly higher than those in the control group （P < 0.05）. NEUT, MONO, FDP, D-dimer, and NIHSS scores were positively correlated with the pre-treatment NETs levels （P < 0.05）. Elevated serum NETs levels were associated with AIS risk factors （P < 0.05）. The combined diagnostic value of NEUT, D-dimer, and NETs for AIS was superior to other indicators. Conclusion NETs are highly expressed in the serum of patients with acute ischemic stroke, and serum NETs have certain value in the auxiliary diagnosis and monitoring of acute ischemic stroke.
- Neutrophil extracellular traps,
- Citrullinated histone H3,
- Neutrophil elastase,
- Myeloperoxidase

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References(31)

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