The Predictive Value of Serum HSP27，CCL22，sTim-3 for the Prognosis of Periodontitis

Yingjie ZHAO; Han GAO; Yiqi ZHENG; Yang CAO; Xin LIU

doi:10.12259/j.issn.2095-610X.S20260313

Volume 47 Issue 3

Mar. 2026

Turn off MathJax

Article Contents

Article Navigation > Journal of Kunming Medical University > 2026 > 47(3): 114-122

Yingjie ZHAO, Han GAO, Yiqi ZHENG, Yang CAO, Xin LIU. The Predictive Value of Serum HSP27，CCL22，sTim-3 for the Prognosis of Periodontitis[J]. Journal of Kunming Medical University, 2026, 47(3): 114-122. doi: 10.12259/j.issn.2095-610X.S20260313

Citation:

Yingjie ZHAO, Han GAO, Yiqi ZHENG, Yang CAO, Xin LIU. The Predictive Value of Serum HSP27，CCL22，sTim-3 for the Prognosis of Periodontitis[J]. Journal of Kunming Medical University, 2026, 47(3): 114-122. doi: 10.12259/j.issn.2095-610X.S20260313

Citation:

PDF( 1007 KB)

The Predictive Value of Serum HSP27，CCL22，sTim-3 for the Prognosis of Periodontitis

doi: 10.12259/j.issn.2095-610X.S20260313

1.
Department of Stomatology
2.
Department of Laboratory Medicine，Beijing Aerospace General Hospital，Beijing 100076
3.
Department of Stomatology，Police Outpatient Department，Beijing Municipal Public Security Bureau，Beijing 100006，China

Received Date: 2025-11-18
Available Online: 2026-04-02
Publish Date: 2026-03-31

Abstract

Abstract

Objective To explore the levels of heat shock protein 27 （HSP27）, chemokine ligand 22 （CCL22）, and soluble T cell immunoglobulin mucin molecule-1 （sTim-3） in the serum of periodontitis patients, analyze their correlation with periodontal clinical indicators and prognosis, and test the predictive efficacy of each biomarker individually and in combination for prognosis. Methods A total of 422 patients with periodontitis admitted to Beijing Aerospace General Hospital from April 2021 to April 2025 were selected, and 415 healthy individuals who underwent physical examinations during the same period were chosen as the control group. Serum and gingival crevicular fluid （GCF） samples were collected from both groups at baseline and at the 6-month follow-up. Levels of HSP27, CCL22, and sTim-3 were measured in serum and GCF. Periodontal parameters, including bleeding index （BI）, gingival index （GI）, attachment loss （AL）, and probing depth （PD）, were recorded concurrently. Differences in these indicators between the two groups were compared, and the correlation between serum and GCF levels of HSP27, CCL22, and sTim-3 was analyzed. The characteristics of HSP27, CCL22, and sTim-3 changes were compared between periodontitis patients with different prognoses. Receiver operating characteristic （ROC） curves were plotted, and the area under the curve （AUC） was calculated to evaluate the predictive value of changes in each indicator for an unfavorable prognosis. Results At baseline and 6-month follow-up, the levels of HSP27, CCL22, and sTim-3 in serum and GCF were significantly higher in the periodontitis group than in the healthy group（P < 0.05）; After 6 months of treatment, the levels of serum and GCF indicators in the periodontitis group significantly decreased compared to baseline（P < 0.05）, while there were no significant differences in various indicators before and after follow-up in the healthy group（P > 0.05）. Pearson correlation analysis showed that the levels of HSP27, CCL22, and sTim-3 in the serum and GCF of patients with periodontitis were significantly positively correlated at both baseline and 6-month follow-up（P < 0.05）. The serum levels of HSP27, CCL22, and sTim-3 in the poor prognosis group were higher than those in the good prognosis group at both baseline and the 6-month follow-up, and the magnitude of post-treatment change in these indicators was significantly lower than those in the good prognosis group（P < 0.05）. ROC curve analysis showed that the changes in HSP27, CCL22, and sTim-3 each had good predictive value for a poor prognosis in periodontitis patients. The AUC of the combined prediction of the three indicators was 0.925, significantly higher than that of a single indicator（P < 0.05）. Conclusion Serum HSP27, CCL22, and sTim-3 are important biological markers for the prognosis of periodontitis. Combined detection of these three markers has significant clinical value for prognostic assessment in periodontitis patients.
- Periodontitis,
- Heat shock protein 27,
- C-C motif chemokine ligand 22,
- Soluble T-cell immunoglobulin and mucin-domain containing-3,
- Prognosis

FullText(HTML)

References(22)

References

[1]	Trindade D, Carvalho R, Machado V, et al. Prevalence of periodontitis in dentate people between 2011 and 2020: A systematic review and meta-analysis of epidemiological studies[J]. J Clin Periodontol, 2023, 50(5): 604-626. doi: 10.1111/jcpe.13769
[2]	Walther K A, Gröger S, Vogler J A H, et al. Inflammation indices in association with periodontitis and cancer[J]. Periodontol 2000, 2024, 96(1): 281-315.
[3]	Mendonça C D, Mata A D S P, Azevedo L F R, et al. Probiotics in the non-surgical treatment of periodontitis: A systematic review and network meta-analysis[J]. BMC Oral Health, 2024, 24(1): 1224. doi: 10.1186/s12903-024-05027-6
[4]	李超, 徐紫薇, 张璇, 等. 血清CCL21、CYTL-1水平与慢性牙周炎患者牙龈卟啉单胞菌感染及牙周临床指标的相关性[J]. 标记免疫分析与临床, 2024, 31(2): 264-269. doi: 10.11748/bjmy.issn.1006-1703.2024.02.014
[5]	Boyd R A, Majumder S, Stiban J, et al. The heat shock protein Hsp27 controls mitochondrial function by modulating ceramide generation[J]. Cell Rep, 2023, 42(9): 113081. doi: 10.1016/j.celrep.2023.113081
[6]	Zou S, Liu B, Feng Y. CCL17, CCL22 and their receptor CCR4 in hematologic malignancies[J]. Discov Oncol, 2024, 15(1): 412. doi: 10.1007/s12672-024-01210-x
[7]	Chen C, Zhao F, Peng J, et al. Soluble Tim-3 serves as a tumor prognostic marker and therapeutic target for CD8⁺ T cell exhaustion and anti-PD-1 resistance[J]. Cell Rep Med, 2024, 5(8): 101686. doi: 10.1016/j.xcrm.2024.101686
[8]	中华口腔医学会牙周病学专业委员会. 重度牙周炎诊断标准及特殊人群牙周病治疗原则的中国专家共识[J]. 中华口腔医学杂志, 2017, 52(2): 67-71.
[9]	李黎, 徐邦勇, 高珺, 等. 米诺环素对固定义齿修复后继发牙周炎的临床疗效[J]. 昆明医科大学学报, 2017, 38(3): 75-78.
[10]	Chapple I L C, Mealey B L, Van Dyke T E, et al. Periodontal health and gingival diseases and conditions on an intact and a reduced periodontium: Consensus report of workgroup 1 of the 2017 World Workshop on the Classification of Periodontal and Peri-Implant Diseases and Conditions[J]. J Periodontol, 2018, 89(Suppl 1): S74-S84. doi: 10.1002/JPER.17-0719
[11]	Linden G J, Lyons A, Scannapieco F A. Periodontal systemic associations: Review of the evidence[J]. J Periodontol, 2013, 84(4 Suppl): S8-S19. doi: 10.1902/jop.2013.1340010
[12]	Tonetti M S, Greenwell H, Kornman K S. Staging and grading of periodontitis: Framework and proposal of a new classification and case definition[J]. J Periodontol, 2018, 89(Suppl 1): S159-S172. doi: 10.1002/JPER.18-0006
[13]	Cortellini P, Cortellini S, Bonaccini D, et al. Modified minimally invasive surgical technique in human intrabony defects with or without regenerative materials-10-year follow-up of a randomized clinical trial: Tooth retention, periodontitis recurrence, and costs[J]. J Clin Periodontol, 2022, 49(6): 528-536. doi: 10.1111/jcpe.13627
[14]	姜文心, 夏晨蕾, 丁弦, 等. 牙周-正畸联合治疗对侵袭性牙周炎患者牙周功能和龈沟液TSLP、IL-33的影响及其预后的影响因素研究[J]. 现代生物医学进展, 2022, 22(20): 3972-3976+3981. doi: 10.13241/j.cnki.pmb.2022.20.034
[15]	张婧, 汪静. 慢性牙周炎患者龈沟液LF、Shh蛋白、TREM-1水平与牙周临床指标和炎性因子的相关性研究[J]. 检验医学与临床, 2023, 20(13): 1859-1863. doi: 10.3969/j.issn.1672-9455.2023.13.008
[16]	Eickholz P, Schröder M, Asendorf A, et al. Effect of nonsurgical periodontal therapy on haematological parameters in grades B and C periodontitis: An exploratory analysis[J]. Clin Oral Investig, 2020, 24(12): 4291-4299. doi: 10.1007/s00784-020-03292-7
[17]	Uttamani J R, Kulkarni V, Valverde A, et al. Dynamic changes in macrophage polarization during the resolution phase of periodontal disease[J]. Immun Inflamm Dis, 2024, 12(10): e70044. doi: 10.1002/iid3.70044
[18]	Moore T C, Pinkerton T S, Petro T M. IRF3 promotes production of IL-6 and nitric oxide but represses CCL22 in RAW_264.7 macrophage cells exposed to lipopolysaccharides in culture[J]. J Inflamm Res, 2025, 18: 1255-1265. doi: 10.2147/JIR.S496930
[19]	Grossman T B, Minis E, Loeb-Zeitlin S E, et al. Soluble T cell immunoglobulin mucin domain 3 (sTim-3) in maternal sera: A potential contributor to immune regulation during pregnancy[J]. J Matern Fetal Neonatal Med, 2021, 34(24): 4119-4122. doi: 10.1080/14767058.2019.1706471
[20]	姜文心, 夏晨蕾, 丁弦, 等. 牙周-正畸联合治疗对侵袭性牙周炎患者牙周功能和龈沟液TSLP、IL-33的影响及其预后的影响因素研究[J]. 现代生物医学进展, 2022, 22(20): 3972-3976+3981. doi: 10.13241/j.cnki.pmb.2022.20.034
[21]	Schröder A, Wagner K, Cieplik F, et al. Impact of phosphorylation of heat shock protein 27 on the expression profile of periodontal ligament fibroblasts during mechanical strain[J]. J Orofac Orthop, 2023, 84(Suppl 2): 143-153.
[22]	Chen L, Qin Y, Lin B, et al. Clinical value of the sTim-3 level in chronic kidney disease[J]. Exp Ther Med, 2022, 24(4): 606. doi: 10.3892/etm.2022.11543

Relative Articles(20)

Relative Articles

[1]	Xiaojian WANG, Junping LIU, Di ZHANG, Qian GUO. Combined Predictive Value of miR-155 and miR-146a in Peripheral Blood Mononuclear Cells for Assessing Poor Prognosis Risk in Patients with Uremia Undergoing Maintenance Hemodialysis. Journal of Kunming Medical University, 2026, 47(4): 1-9.
[2]	Yang LI, Wen JIANG, Rong LIU, Chaomin LIU, Longyu XU, Wenjing ZHANG. Bioinformatics-based Identification of Cuproptosis Genes Associated with Prognosis and Immune Microenvironment in Lung Cancer. Journal of Kunming Medical University, 2025, 46(4): 29-35. doi: 10.12259/j.issn.2095-610X.S20250405
[3]	Zuohua WANG, Rongfeng WU, Xiaoyan ZHANG, Huiping WANG. Relationship of Serum IL-27，CysC，GAPDH Antibodies with Disease Severity and Prognosis in Children with Neuromyelitis Optica Spectrum Disorder. Journal of Kunming Medical University, 2025, 46(7): 146-154. doi: 10.12259/j.issn.2095-610X.S20250718
[4]	Zhi XIE, Lang YANG, Xiaolong ZHANG. Explorations of the Clinical Pathology and Prognosis of Gastric Cancer Based on Systemic Immune Inflammation Index and Ferritin Expression. Journal of Kunming Medical University, 2025, 46(9): 129-136. doi: 10.12259/j.issn.2095-610X.S20250915
[5]	Yanfei HUANG, Hongbin YU, Lingyun YIN, Jing LIANG, Changquan LI, Dehong LI, Jinyuan WANG, Qian OUYANG. Regulatory Mechanism of Keap1/Nfe2L2 on Osteogenic Differentiation in Periodontitis. Journal of Kunming Medical University, 2025, 46(7): 26-37. doi: 10.12259/j.issn.2095-610X.S20250704
[6]	Chunyan LIU, Bingqing CHANG, Chao LI, Xin REN, Xiaoqin LIU. The Relationship between T Lymphocyte Subsets and Pathological Characteristics of Acute Myeloid Leukemia and the Value of Predicting Chemotherapy Prognosis. Journal of Kunming Medical University, 2024, 45(5): 116-122. doi: 10.12259/j.issn.2095-610X.S20240518
[7]	Qi NIE, Li LIU, Yue TIAN, Xiaoyan MAO, Qulian GUO, Xin TIAN. Abnormal Clinical Features and Prognosis of ACTH in Pediatric Acute B Lymphocytic Leukemia. Journal of Kunming Medical University, 2024, 45(10): 75-84. doi: 10.12259/j.issn.2095-610X.S20241012
[8]	Lizhu ZHAO, Ying DONG, Yue DENG, Lihua YANG. Correlation between Epithelial Cell Related Genes and Prognosis of Patients with Ovarian Cancer based on Single Cell Sequencing. Journal of Kunming Medical University, 2024, 45(4): 9-16. doi: 10.12259/j.issn.2095-610X.S20240402
[9]	Lili YANG, Ting ZHOU, Xiaobin REN, Jianhua WU, Yi PENG, Yanqing SHUI. Prospective Clinical Study of Combined Treatment of Periodontal Orthodontics for Chronic Periodontitis. Journal of Kunming Medical University, 2024, 45(3): 54-58. doi: 10.12259/j.issn.2095-610X.S20240308
[10]	Ruiting ZHANG, Bin YAN, Zuyan LI, Jinmin SUN, Jin YAO. Relations between Peripheral Blood Blast Clearance Time and Albumin Level at Initial Diagnosis during Induction Chemotherapy and Genetic Mutations and Prognosis in Patients with Acute Myeloid Leukemia. Journal of Kunming Medical University, 2024, 45(8): 117-124. doi: 10.12259/j.issn.2095-610X.S20240817
[11]	Xinyi MEN, Jing ZHAO, Yongchun SHEN, Hui JI, Xiuxia WANG. The Relationship between Peripheral Blood Immunoglobulin，Erythrocyte Sedimentation Rate，Homocysteine and the Degree of Central Nervous System Vasculitis in Children and Their Influence on Prognosis. Journal of Kunming Medical University, 2024, 45(12): 122-128. doi: 10.12259/j.issn.2095-610X.S20241217
[12]	Le ZHAN, Ruiyu MA, Ni WAN, Liwen MO, Huiying CHEN, Li ZHANG. Effects of Puerarin on Th17/Treg Cell Immune Homeostasis and the Expression of Related Transcription Factors in Rats with Periodontitis. Journal of Kunming Medical University, 2022, 43(11): 36-43. doi: 10.12259/j.issn.2095-610X.S20221129
[13]	Xu ZHENG, Chen XIE, Rao FU, Yu-zhi WU, Zhu-ling GUO. A Survey on the Cognition of the Relationship between Periodontitis and Diabetes Mellitus in Type 2 Diabetes Patients and Endocrinologists. Journal of Kunming Medical University, 2021, 42(3): 89-93. doi: 10.12259/j.issn.2095-610X.S20210323
[14]	Bi Shan , Luo Hao Dan , Chen Shao Chun , Li Guo Ping . . Journal of Kunming Medical University, 2020, 41(07): 165-169.
[15]	Jian-pei SU, Wei-meng TIAN, Jun GU, Mi-yu HE. Association between C-reactive Protein/Albumin Ratio and Long-term Prognosis in Elderly Patients with Heart Failure. Journal of Kunming Medical University, 2020, 41(12): 128-132. doi: 10.12259/j.issn.2095-610X.S20201236
[16]	Wu Wen Juan . Combination of Heart-type Fatty Acid-binding Protein and Simple Pulmonary Embolism Severity Index for the Assessment of the Short-term Prognosis in Patients with Acute Pulmonary Embolism. Journal of Kunming Medical University,
[17]	Wang Xiang Yun . . Journal of Kunming Medical University,
[18]	Xu Jian Xin . . Journal of Kunming Medical University,
[19]	Zhang Yi Fan . . Journal of Kunming Medical University,
[20]	Liu Jian Gang . . Journal of Kunming Medical University,