The Relationship between Peripheral Blood MiR-409-3p，MiR-6861-5p and the Prognosis of TACE in Patients with Hepatocellular Carcinoma

Tong HUANG; Jie HU; Haiting YUAN; Jiahui LI; Wuhua LIN

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Tong HUANG, Jie HU, Haiting YUAN, Jiahui LI, Wuhua LIN. The Relationship between Peripheral Blood MiR-409-3p，MiR-6861-5p and the Prognosis of TACE in Patients with Hepatocellular Carcinoma[J]. Journal of Kunming Medical University.

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The Relationship between Peripheral Blood MiR-409-3p，MiR-6861-5p and the Prognosis of TACE in Patients with Hepatocellular Carcinoma

Department of Oncology，Sichuan Provincial Armed Police Corps Hospital，Leshan Sichuan 614000，China

Received Date: 2025-11-24
Available Online: 2026-03-09

Abstract

Abstract

Objective To investigate the relationship between peripheral blood miR-409-3p, miR-6861-5p and the prognosis of patients with Hepatocellular carcinoma （HCC） after Transcatheter arterial chemoembolization （TACE）. Methods Two hundred HCC patients admitted to the Sichuan Provincial Armed Police Corps Hospital from January 2019 to April 2022 were selected as the HCC group and treated with TACE. Another 200 healthy subjects were selected in the same period as the control group. The expression levels of miR-409-3p and miR-6861-5p in peripheral blood were compared between the two groups and among HCC patients with different clinical characteristics. The expression levels of peripheral blood miR-409-3p and miR-6861-5p were compared between the two groups and patients were followed for 3 years to assess TACE treatment outcomes. Expression levels and changes of peripheral blood miR-409-3p and miR-6861-5p were compared among patients with different prognoses and treatment responses. COX regression was used to analyze the prognostic factors. Receiver operating characteristic （ROC） curves were plotted to analyze the efficacy of miR-409-3p and miR-6861-5p in predicting the prognosis of TACE treatment in HCC patients. Kaplan-Meier survival curves were used to analyze the relationship between miR-409-3p, miR-6861-5p and the prognosis of TACE in HCC patients. Results Compared with the control group, the expression level of miR-409-3p in peripheral blood in the HCC group was lower, while the expression level of miR-6861-5p was higher （P < 0.05）. Of the 200 patients followed for 3 years, 9 patients were lost to follow-up. Among the remaining patients, 101 （52.88%） died from HCC. The results of multivariate COX regression analysis showed that the number of tumors, portal vein tumor thrombus, initial TACE response, subsequent systemic treatment, and the expression levels of miR-409-3p and miR-6861-5p in peripheral blood were independent prognostic factors for HCC patients （P < 0.05）. After adjusting for the number of tumors, portal vein tumor thrombus, initial TACE response, and subsequent systemic treatment, the expression of ΔmiR-409-3p and ΔmiR-6861-5p was still independently associated with the prognosis of HCC patients （P < 0.05）. ROC curve showed that the Area under the curve （AUC） of ΔmiR-409-3p and ΔmiR-6861-5p alone in predicting the prognosis of TACE was 0.766 and 0.750, respectively. The area under the curve of the combined prediction of the two markers yielded an AUC of 0.863, which was significantly larger than that of either index alone （P < 0.05）. Conclusion Peripheral blood miR-409-3p and miR-6861-5p are important potential regulatory molecules for HCC, and their dynamic changes before and after treatment （Δ value） are closely related to TACE efficacy and patient survival. The predictive model constructed by the combined detection of the two Δ values has a high clinical transformation potential, which is expected to be used for the screening of high-risk patients before TACE treatment and the early evaluation and dynamic monitoring of the efficacy after treatment, so as to provide a new evidence-based basis for the realization of individualized treatment management of HCC.
- Hepatocellular carcinoma,
- miR-409-3p,
- miR-6861-5p,
- Transcatheter arterial chemoembolization,
- Prognosis

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