Inhibition of Breast Cancer by Ethanol Extract of Hedyotis diffusa through Mitochondrial Apoptosis Mediated by Cytochrome c/Caspase-9

Xiaoxiao LIAN; Tao HAN; Kunmeng CUI; Yan WANG; Yongfang LIU; Tian GUO; Ruixue YANG; Ya WANG

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Article Navigation > Journal of Kunming Medical University > 2025 > Accepted Manuscript

Xiaoxiao LIAN, Tao HAN, Kunmeng CUI, Yan WANG, Yongfang LIU, Tian GUO, Ruixue YANG, Ya WANG. Inhibition of Breast Cancer by Ethanol Extract of Hedyotis diffusa through Mitochondrial Apoptosis Mediated by Cytochrome c/Caspase-9[J]. Journal of Kunming Medical University.

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Inhibition of Breast Cancer by Ethanol Extract of Hedyotis diffusa through Mitochondrial Apoptosis Mediated by Cytochrome c/Caspase-9

Department of Traditional Chinese Medicine，Handan Central Hospital，Handan Hebei 056000，China

Received Date: 2024-01-20

Abstract

Abstract

Objective To investigate the effects and potential mechanisms of Hedyotis diffusa extract （HDE） on breast cancer cell proliferation, migration and mitochondrial damage through the cytochrome c （Cyt-c）/cysteine aspartate aminotransferase-9 （Caspase-9） pathway. Methods Cell Counting Kit-8 （CCK-8） was used to detect breast cancer cell viability in each group. MCF7 breast cancer cells were continuously cultured and divided into control group, HDE group, and Cyt-c overexpression group. Immunofluorescence staining was used to detect the protein levels of Cyt-c and Caspase-9 in MCF7 cells. 5-ethynyl-2'-deoxyuridine （EdU） kit was used to detect MCF7 cell proliferation ability. Scratch assay was used to detect MCF7 cell migration ability. Flow cytometry was used to detect mitochondrial membrane potential levels in MCF7 cells. Enzyme-linked immunosorbent assay （ELISA） kit was used to detect adenosine triphosphate （ATP） content in MCF7 cells. Results Compared with the control group, the cell viability of BT474, SKBr-3, ZR-75-30, MCF7 and MDA-MB-453 cells in the HDE group was decreased （P < 0.05）. MCF7 breast cancer cells were selected for subsequent studies. Compared with the control group, the HDE group showed increased expression of Cyt-c and Caspase-9 in MCF7 cells （P < 0.05）, decreased cell proliferation and migration ability （P < 0.05）, reduced mitochondrial membrane potential, and decreased ATP content （P < 0.05）. Compared with the control group, the Cyt-c overexpression group showed decreased cell proliferation and migration ability （P < 0.05）, reduced mitochondrial membrane potential, and decreased ATP content （P < 0.05）. Conclusion HDE may inhibit breast cancer cell, and promote mitochondrial damage by activating Cyt-c/Caspase-9 pathway.
- Breast cancer,
- Hedyotis diffusa extract,
- Cyt-c/Caspase-9 pathway,
- Mitochondrial damage

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References(22)

References

[1]	Katsura C, Ogunmwonyi I, Kankam H K, et al. Breast cancer: Presentation, investigation and management[J]. Br J Hosp Med, 2022, 83(2): 1-7.
[2]	Wilkinson L, Gathani T. Understanding breast cancer as a global health concern[J]. Br J Radiol, 2022, 95(1130): 20211033. doi: 10.1259/bjr.20211033
[3]	Tao X, Li T, Gandomkar Z, et al. Incidence, mortality, survival, and disease burden of breast cancer in China compared to other developed countries[J]. Asia Pac J Clin Oncol, 2023, 19(6): 645-654. doi: 10.1111/ajco.13958
[4]	Moubadder L, Collin L J, Nash R, et al. Drivers of racial, regional, and socioeconomic disparities in late-stage breast cancer mortality[J]. Cancer, 2022, 128(18): 3370-3382. doi: 10.1002/cncr.34391
[5]	Wolf P, Schoeniger A, Edlich F. Pro-apoptotic complexes of BAX and BAK on the outer mitochondrial membrane[J]. Biochim Biophys Acta Mol Cell Res, 2022, 1869(10): 119317. doi: 10.1016/j.bbamcr.2022.119317
[6]	Xin Y, Sun Z, Liu J, et al. Nanomaterial-mediated low-temperature photothermal therapy via heat shock protein inhibition[J]. Front Bioeng Biotechnol, 2022, 10: 1027468. doi: 10.3389/fbioe.2022.1027468
[7]	Deepak K, et al. Exploring the role of mitophagy-related genes in breast cancer progression and metastasis: A systematic review[J]. Int J Mol Sci, 2024, 25(3): 页码?
[8]	Meng M, Yang Q, Ouyang Z, et al. Ampelopsin induces MDA-MB-231 cell cycle arrest through cyclin B1-mediated PI3K/AKT/mTOR pathway in vitro and in vivo[J]. Acta Pharm, 2023, 73(1): 75-90. doi: 10.2478/acph-2023-0005
[9]	Hung H Y, Cheng K C, Kuo P C, et al. Chemical constituents of Hedyotis diffusa and their anti-inflammatory bioactivities[J]. Antioxidants, 2022, 11(2): 335. doi: 10.3390/antiox11020335
[10]	Zhang R, Ma C, Wei Y, et al. Isolation, purification, structural characteristics, pharmacological activities, and combined action of Hedyotis diffusa polysaccharides: A review[J]. Int J Biol Macromol, 2021, 183: 119-131. doi: 10.1016/j.ijbiomac.2021.04.139
[11]	Li Z, Li J, Liu X, et al. Ethyl acetate fraction from Hedyotis Diffusa plus Scutellaria Barbata inhibits the progression of breast cancer via targeting LMO1 and AKT/mtor signaling pathway[J]. Comb Chem High Throughput Screen, 2024, 27(12): 1735-1744. doi: 10.2174/1386207326666230913105858
[12]	Lewis S M, Callaway M K, Dos Santos C O. Clinical applications of 3D normal and breast cancer organoids: A review of concepts and methods[J]. Exp Biol Med, 2022, 247(24): 2176-2183. doi: 10.1177/15353702221131877
[13]	Wang X, Wang C, Guan J, et al. Progress of breast cancer basic research in China[J]. Int J Biol Sci, 2021, 17(8): 2069-2079. doi: 10.7150/ijbs.60631
[14]	Xia C, Dong X, Li H, et al. Cancer statistics in China and United States, 2022: Profiles, trends, and determinants[J]. Chin Med J, 2022, 135(5): 584-590. doi: 10.1097/CM9.0000000000002108
[15]	Zhai J, Wu Y, Ma F, et al. Advances in medical treatment of breast cancer in 2022[J]. Cancer Innov, 2023, 2(1): 1-17. doi: 10.1002/cai2.46
[16]	Rao Malla R, Marni R, Kumari S, et al. Microbiome assisted tumor microenvironment: Emerging target of breast cancer[J]. Clin Breast Cancer, 2022, 22(3): 200-211. doi: 10.1016/j.clbc.2021.09.002
[17]	Pessoa J. Cytochrome c in cancer therapy and prognosis[J]. Biosci Rep, 2022, 42(12): BSR20222171. doi: 10.1042/BSR20222171
[18]	Chen Q, Li Q, Liang Y, et al. Natural exosome-like nanovesicles from edible tea flowers suppress metastatic breast cancer via ROS generation and microbiota modulation[J]. Acta Pharm Sin B, 2022, 12(2): 907-923. doi: 10.1016/j.apsb.2021.08.016
[19]	Kaur H, Singh A, Kaur K, et al. 4-methylthiobutyl isothiocyanate synergize the antiproliferative and pro-apoptotic effects of paclitaxel in human breast cancer cells[J]. Biotechnol Genet Eng Rev, 2024, 40(4): 3780-3804. doi: 10.1080/02648725.2022.2162232
[20]	Zou Y, Xu Y, Chen X, et al. Research progress on leucine-rich alpha-2 glycoprotein 1: A review[J]. Front Pharmacol, 2022, 12: 809225. doi: 10.3389/fphar.2021.809225
[21]	Lin Y, Zhao Y, Chen M, et al. CYD0281, a Bcl-2 BH4 domain antagonist, inhibits tumor angiogenesis and breast cancer tumor growth[J]. BMC Cancer, 2023, 23(1): 479. doi: 10.1186/s12885-023-10974-4
[22]	Rodrigues J A, Pires B R B, de Amorim I S S, et al. STAT3 regulates the redox profile in MDA-MB-231 breast cancer cells[J]. Cell Biochem Biophys, 2024, 82(4): 3507-3516. doi: 10.1007/s12013-024-01439-x

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