Expression of PG Ⅰ/PG Ⅱ，NAP1L1，SERPINB5 in Serum of Gastric Cancer Patients and Cox Regression Analysis on Prognosis

Huanhuan LIN; Dongguang YANG; Yanping LI; Haiyun LI; Jinqiao HAN

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Huanhuan LIN, Dongguang YANG, Yanping LI, Haiyun LI, Jinqiao HAN. Expression of PG Ⅰ/PG Ⅱ，NAP1L1，SERPINB5 in Serum of Gastric Cancer Patients and Cox Regression Analysis on Prognosis[J]. Journal of Kunming Medical University.

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Expression of PG Ⅰ/PG Ⅱ，NAP1L1，SERPINB5 in Serum of Gastric Cancer Patients and Cox Regression Analysis on Prognosis

1.
Department of General Surgery, Zhuozhou Hospital，Zhuozhou Hebei 072750
2.
Department of Obstetrics and Gynaecology, Zhuozhou Traditional Chinese Medicine Hospital，Zhuozhou Hebei 072750，China

Received Date: 2025-05-27
Available Online: 2025-08-13

Abstract

Abstract

Objective To explore the expression of pepsinogen I/II （PG Ⅰ/PG Ⅱ）, nucleosome assembly protein 1 like protein 1 （NAP1L1）, and SERPINB5 in the serum of gastric cancer patients and their correlation with prognosis. Methods From February 2019 to February 2022, 200 gastric cancer patients admitted to Zhuozhou Hospital were served as the gastric cancer group and another 100 patients with benign gastric lesions who received the treatment at Zhuozhou Hospital during the same period were served as the benign group, with 200 healthy adults as the control group. Chemiluminescence and ELISA methods were used to detect the serum PG Ⅰ/PG Ⅱ, NAP1L1, and SERPINB5. ROC curve was used to explore the diagnostic value. Kaplan-Meier method was used to explore the survival curve. Moreover, multivariate Cox method was used to analyze the factors that affected the prognosis. Results Compared with the control group, the benign group and gastric cancer group had the lower serum PG Ⅰ/PG Ⅱ and higher serum NAP1L1 and SERPINB5, and the difference was statistically significant （P < 0.05）. Compared with the benign group, the gastric cancer group had the lower serum PG Ⅰ/PG Ⅱ and higher serum NAP1L1 and SERPINB5, and the difference was statistically significant （P < 0.05）. PG I/PG II, NAP1L1, and SERPINB5 were all influential factors in gastric carcinogenesis （P < 0.05）. The AUC values of serum PG Ⅰ/PG Ⅱ, NAP1L1, and SERPINB5 alone in the diagnosis of gastric cancer were 0.821, 0.808, and 0.833, respectively. The AUC of the combination of the three was 0.916, indicating that their combined diagnostic value was superior （Z = 3.142, 3.896, 3.114, P < 0.05）. During the 3-year follow-up period of gastric cancer patients, 57 cases died, accounting for 28.50% （57/200）, and 143 cases survived. Patients with the high expression of PG Ⅰ/PG Ⅱ had a higher 3-year overall survival rate after the surgery compared to those with low expression, and the difference was statistically significant （χ² = 7.830, P < 0.05）; and patients with the low expression of NAP1L1 and SERPINB5 had a higher 3-year overall survival rate after the surgery compared to those with high expression, and the difference was statistically significant （χ² = 8.612, 13.321, P < 0.05）. The serum PG Ⅰ/PG Ⅱ levels in the death group were lower than those in the survival group, and the serum NAP1L1, SERPINB5 levels were higher in patients with preoperative lymph node metastasis and TNM stage III-IV than those in the survival group, and the difference was statistically significant （P < 0.05）. Elevated level of PG Ⅰ/PG Ⅱ was a protective factor for the prognosis of gastric cancer patients, while preoperative lymph node metastasis, elevated levels of NAP1L1 and SERPINB5 were risk factors affecting the prognosis of gastric cancer patients （P < 0.05）. Conclusion Serum PG I /PG II levels are decreased and NAP1L1 and SERPINB5 levels are increased in gastric cancer patients, and NAP1L1 and SERPINB5 are risk factors affecting the prognosis of gastric cancer patients, while PG I /PG II is a protective factor.
- Gastric cancer,
- Pepsinogen I/II,
- Nucleosome assembly protein 1 like protein 1,
- SERPINB5,
- Prognosis

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