In Vitro Inhibition of Coxsackievirus by Blumea Samifera （L.）DC Extracts

Huang LI; Rongcheng WEN; Li CHAI; Xia LI; Jinyan JIA; Zhen CHEN

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Huang LI, Rongcheng WEN, Li CHAI, Xia LI, Jinyan JIA, Zhen CHEN. In Vitro Inhibition of Coxsackievirus by Blumea Samifera （L.）DC Extracts[J]. Journal of Kunming Medical University.

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Huang LI, Rongcheng WEN, Li CHAI, Xia LI, Jinyan JIA, Zhen CHEN. In Vitro Inhibition of Coxsackievirus by Blumea Samifera （L.）DC Extracts[J]. Journal of Kunming Medical University.

Huang LI, Rongcheng WEN, Li CHAI, Xia LI, Jinyan JIA, Zhen CHEN. In Vitro Inhibition of Coxsackievirus by Blumea Samifera （L.）DC Extracts[J]. Journal of Kunming Medical University.

Citation:

Huang LI, Rongcheng WEN, Li CHAI, Xia LI, Jinyan JIA, Zhen CHEN. In Vitro Inhibition of Coxsackievirus by Blumea Samifera （L.）DC Extracts[J]. Journal of Kunming Medical University.

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In Vitro Inhibition of Coxsackievirus by Blumea Samifera （L.）DC Extracts

1.
School of Rehabilitation
2.
School of Pharmaceutical Science，Kunming Medical University，Kunming Yunnan 650500
3.
Guizhou Holy Pharmaceutical Co. Ltd，Guiyang Guizhou 550018，China

Received Date: 2024-08-01
Available Online: 2025-02-18

Abstract

Abstract

Objective To investigate the in vitro antiviral effects of Blumea balsamifera（L.）DC. extract against Coxsackievirus B5 （CVB5）. Methods A series of dilutions of Coxsackievirus were prepared and co-cultured with RD cells to determine the TCID50 value. Subsequently, different concentrations of the extract were added to a 96-well plate containing RD cells to evaluate their impact on cell viability. The ability of Blumea balsamifera extract to inhibit Coxsackievirus was further observed in the 96-well plate containing RD cells and the extract. Results The TCID₅₀ value of Coxsackie virus solution was 10^−7.67. The inhibition rate of Blumea balsamifera extract against Coxsackievirus increased with concentration, with an IC50 value of 7.26 mg/L. At a concentration of 50 mg/L, the extract caused a decrease in RD cell viability（P < 0.05）, but within the concentration range of 6.25 to 50 mg/L, it increased the viability of virus-infected RD cells（P < 0.05）, with a selectivity index （SI） exceeding 6.89. Conclusion Blumea balsamifera（L.）DC. extract exhibits in vitro activity against Coxsackievirus.
- Blumea balsamifera（L.）DC.,
- Antiviral agents,
- Coxsackie virus

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References

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