Screening of Candidate Strains for Coxsackievirus Group A Virus Type 16 Vaccine

Jie PENG; Jie LIU; Lingfeng HU; Yun LIAO; Qihan LI; Shengtao FAN

doi:10.12259/j.issn.2095-610X.S20250402

Volume 46 Issue 4

Apr. 2025

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Jie PENG, Jie LIU, Lingfeng HU, Yun LIAO, Qihan LI, Shengtao FAN. Screening of Candidate Strains for Coxsackievirus Group A Virus Type 16 Vaccine[J]. Journal of Kunming Medical University, 2025, 46(4): 8-13. doi: 10.12259/j.issn.2095-610X.S20250402

Citation:

Jie PENG, Jie LIU, Lingfeng HU, Yun LIAO, Qihan LI, Shengtao FAN. Screening of Candidate Strains for Coxsackievirus Group A Virus Type 16 Vaccine[J]. Journal of Kunming Medical University, 2025, 46(4): 8-13. doi: 10.12259/j.issn.2095-610X.S20250402

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Screening of Candidate Strains for Coxsackievirus Group A Virus Type 16 Vaccine

doi: 10.12259/j.issn.2095-610X.S20250402

Institute of Medical Biology，Chinese Academy of Medical Sciences & Peking Union Medical College，Kunming Yunnan 650118，China

Received Date: 2024-12-10
Available Online: 2025-04-05
Publish Date: 2025-04-25

Abstract

Abstract

Objective To screen Coxsackievirus A16 （CA16） vaccine candidate strains and provide preliminary basis for the development of vaccine against hand, foot and mouth disease （HFMD）. Methods Cell inoculation and continuous passage were performed on the 35 clinical specimens of patients with hand, foot, and mouth disease from different areas of Kunming. The harvested solution with cytopathic effects was identified using CA16 standard serum. Positive samples were screened for vaccine strains through the virus titer determination, plaque purification, VP1 sequence homology and evolutionary analysis. Results Among the 35 collected clinical oropharyngeal swab samples, 23 showed cellular lesions, and 10 were identified as CA16 virus by serology, with a positive rate of 43.5%. After the adaptive cultivation, the virus titers of 10 strains gradually increased, but stabilized at 7.0 lgCCID50 from 48 hours onwards. The plaque of the strain showed that all strains can form plaques of 0.2～0.8 cm, showing the regular differences, and the size of KM/M08 plaques was relatively uniform. All strains were B1b type, with nucleotide homology ranging from 93.3～100%. Among them, KM/M08 had the highest homology with other strains, ranging from 95.2～100%. Conclusion Through the analysis of virus proliferation kinetics curve, gene homology, and evolution, KM/M08 meets the expected screening criteria in all aspects of evaluation and is proposed as a vaccine candidate strain for further researches.
- Hand-foot-and-mouth disease,
- CA16,
- Vaccine strains,
- Evolutionary analysis

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References(20)

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