Activation of γ-Aminobutyric Acid Type A Receptor Attenuates Oxygen-Glucose Deprivation/Reoxygenation-Induced Astrocyte Injury

Hongxia HE; Tingting CHEN; Fei HUANG; Junwu LIU; Hongli ZHOU; Lei XU

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Article Navigation > Journal of Kunming Medical University > 2025 > Accepted Manuscript

Hongxia HE, Tingting CHEN, Fei HUANG, Junwu LIU, Hongli ZHOU, Lei XU. Activation of γ-Aminobutyric Acid Type A Receptor Attenuates Oxygen-Glucose Deprivation/Reoxygenation-Induced Astrocyte Injury[J]. Journal of Kunming Medical University.

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Activation of γ-Aminobutyric Acid Type A Receptor Attenuates Oxygen-Glucose Deprivation/Reoxygenation-Induced Astrocyte Injury

1.
Meishan Hospital，West China Hospital of Sichuan University，Meishan Sichuan 620000
2.
Department of Anesthesiology，Meishan People’s Hospital，Meishan Sichuan 620000，China

Abstract

Abstract

Objective To investigate the protective role and potential mechanisms of γ-aminobutyric acid type A receptor （GABA_AR） against oxygen-glucose deprivation/reoxygenation （OGD/R）-induced astrocyte injury. Methods C8-D1A astrocytes were used to establish an OGD/R injury model, which was randomly divided into four groups: the normal control group （NC）, the OGD/R model group, the GABA_AR agonist CL218872 intervention group （OGD/R+CL218872）, and the inhibitor MRK-016 intervention group （OGD/R+MRK-016）. Cell proliferation was assessed by EdU staining, apoptosis was analyzed by flow cytometry, GABA_AR and glial fibrillary acidic protein （GFAP） expression were examined by immunofluorescence staining, and brain-derived neurotrophic factor （BDNF） expression was determined by Western blotting. Results Compared with the OGD/R group, the OGD/R+CL218872 group showed significantly increased cell proliferation rate （P < 0.05） , markedly decreased apoptosis rate（P < 0.01）, upregulated expression of both GABA_AR and GFAP（P < 0.05） significantly elevated BDNF protein expression level（P < 0.05）, and significantly reduced levels of inflammatory factors IL-6 （P < 0.01） and TNF-α （P < 0.05）. The OGD/R+MRK-016 group showed opposite changes in all parameters. Conclusion GABA_AR activation can attenuate OGD/R-induced astrocyte injury by promoting astrocyte proliferation, inhibiting apoptosis and inflammatory response, and upregulating the expression of BDNF and GFAP.
- γ-aminobutyric acid type A receptor,
- Astrocytes,
- Oxygen-glucose deprivation/reoxygenation,
- Synaptic remodeling,
- Apoptosis

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References(36)

References

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