Effect of TGF-β2 and geneX on the Proliferation and Osteogenic Differentiation of BrdU-labeled Bone Mesenchymal Stem Cells

Hu Zheng Xiong

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Hu Zheng Xiong . Effect of TGF-β2 and geneX on the Proliferation and Osteogenic Differentiation of BrdU-labeled Bone Mesenchymal Stem Cells[J]. Journal of Kunming Medical University, 2016, 37(02).

Citation:

Hu Zheng Xiong . Effect of TGF-β2 and geneX on the Proliferation and Osteogenic Differentiation of BrdU-labeled Bone Mesenchymal Stem Cells[J]. Journal of Kunming Medical University, 2016, 37(02).

Hu Zheng Xiong . Effect of TGF-β2 and geneX on the Proliferation and Osteogenic Differentiation of BrdU-labeled Bone Mesenchymal Stem Cells[J]. Journal of Kunming Medical University, 2016, 37(02).

Citation:

Hu Zheng Xiong . Effect of TGF-β2 and geneX on the Proliferation and Osteogenic Differentiation of BrdU-labeled Bone Mesenchymal Stem Cells[J]. Journal of Kunming Medical University, 2016, 37(02).

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Effect of TGF-β2 and geneX on the Proliferation and Osteogenic Differentiation of BrdU-labeled Bone Mesenchymal Stem Cells

Hu Zheng Xiong

(1)Dept. of Orthopedics,Diannan Central Hospital of Honghe State/The 5th Affiliated Hospital of Kunming Medical University,Gejiu Yunnan 661000;2)Dep. of Orthopedics,The 1st Affiliated Hospital of Kunming Medical University,Kunming Yunnan 650032;3)Armed Police Corps Hospital of Yunnan Province, Kunming Yunnan 650000,China)

Abstract

Abstract

［Abstract］Objective To study the effect of TGF-β2 and geneX on the proliferation and osteogenic differentiation of BrdU-labeled Bone Mesenchymal Stem Cells. Methods　The whole bone adherent culture method was used to isolate，passage and identify bone mesenchymal stem cells. After grouped induction culture， the morphology， growth kinetics （Methyl Thiazolyl Tetrazolium，MTT）， alkaline phosphatase （ALP） and calcium quantification of BMSCs were observed and the osteogenic differentiation was observed by Von-Kossa staining. Results（1） The absorbance value（OD）of BMSCs in four groups showed statistically significant differences among different cell groups（P <0.05）. （2）The alkaline phosphatase（ALP）and calcium quantitative test results after osteogenesis induction showed statistically significant differences among different cell groups（P <0.05）. （3） Cell Von-Kossa staining showed that large amounts of calcium nodules in group D， more calcium nodules in group C，partial calcium nodules group B， a small amount of calcium nodules in group A， and no calcium nodules in control group. Conclusions （1）TGF-β2 can promote the proliferation of bone mesenchymal stem cells and induce them to differentiate into osteoblasts. （2） GeneX can promote bone mesenchymal stem cells to differentiate into bone cells，and osteogenic differentiation is more obvious in the cytokine TGF-β2 joint action.
- ［Key words］Bone mesenchymal dtem vells；TGF-β2；Gene X；Cell proliferation；Osteogenic differentiation,

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