An Analysis of Movement Function of SD Rats' Spinal Cord Injury After Joint Therapy Using NT-3-HUMSCs and SOCS3 Gene Silencing

Bai Gang; Zhang Hong Tian; Lai Jun; Luo Lin; Zuo Pin; Fan Yao Dong

Article Contents

Article Navigation > Journal of Kunming Medical University > 2018 > 39(02): 15-20

Bai Gang , Zhang Hong Tian , Lai Jun , Luo Lin , Zuo Pin , Fan Yao Dong . An Analysis of Movement Function of SD Rats' Spinal Cord Injury After Joint Therapy Using NT-3-HUMSCs and SOCS3 Gene Silencing[J]. Journal of Kunming Medical University, 2018, 39(02): 15-20.

Citation:

Bai Gang , Zhang Hong Tian , Lai Jun , Luo Lin , Zuo Pin , Fan Yao Dong . An Analysis of Movement Function of SD Rats' Spinal Cord Injury After Joint Therapy Using NT-3-HUMSCs and SOCS3 Gene Silencing[J]. Journal of Kunming Medical University, 2018, 39(02): 15-20.

Citation:

Bai Gang , Zhang Hong Tian , Lai Jun , Luo Lin , Zuo Pin , Fan Yao Dong . An Analysis of Movement Function of SD Rats' Spinal Cord Injury After Joint Therapy Using NT-3-HUMSCs and SOCS3 Gene Silencing[J]. Journal of Kunming Medical University, 2018, 39(02): 15-20.

PDF( 3322 KB)

An Analysis of Movement Function of SD Rats' Spinal Cord Injury After Joint Therapy Using NT-3-HUMSCs and SOCS3 Gene Silencing

Funds:

基金：云南省教育厅科学研究基金重点科研基金资助项目 (2014Z054);

Received Date: 2017-09-21

Abstract

Abstract

Objective To achieve the purpose of promoting movement function of the injury nerve by using the joint therapy of NT-3-HUMSCs and SOCS3 gene silencing on SD rats' spinal cord injury. Methods (1) We used adherence method in vitro human umbilical cord-derived mesenchymal cells ( HUMSC) during separation, purification and identification. (2) Then constructed NT-3 gene eukaryotic expression vector, which was transfected into its HUMSC, and constructed NT-3-HUMSC cell survival in vitro assay conditions and NT-3 expression. (3) We selected specific targets for SOCS3 screening and for sequence homology analysis.A negative control group was established. siRNA was designed and synthesized in vitro detection. (4) SD rats with spinal cord injury model were divided into two categories: (1) sham group with 10 rats; (2) T12 whole spinal cord injury model with 40 rats. The 40 rats were randomly divided into four groups with 10 rats in each group (saline treatment group, siRNA + NT-3-HUMSCs treatment group, NT-3-HUMSCs treatment group and siRNA treated group) . Motor function of the rats were evaluated respectively in 1, 2 and 3 months after the modeling was established successfully.Results (1) siRNA + NT-3-HUMSCs treatment group's BBB scores was significantly higher than NT-3-HUMSCs, SOCS3-siRNA and physiological saline groups (P <0.05) . (2) The grid climbing experiments showed that the neural functional recovery performed better in siRNA + the NT-3-HUMSCs treatment group compared to the NT-3-HUMSCs, SOCS3-siRNA and physiological saline groups (P <0.05) . Conclusion The NT-3-HUMSCs joint SOCS3 gene silencing in the treatment of SD rat spinal cord injury can improve the motor function of SD rat spinal cord injury.
- Spinal cord injury,
- SOCS3,
- siRNA,
- NT-3 gene,
- Gene transfection

FullText(HTML)

References(18)

References

[1]	[1]WALTHERS C M, SEIDLITS S K.Gene delivery strategies to promote spinal cord repair[J].Biomarker Insights, 2015, 10 (Suppl 1) :11.
[2]	[2]HAGEN E M, REKAND T, GILHUS N E, et al.Traumatic spinal cord injuries-incidence, mechanisms and course[J].Tidsskrift for Den Norske Laegeforening:Tidsskrift for Praktisk Medicin, Ny Raekke, 2012, 132 (7) :831-837.
[3]	[3]RAMER L M, RAMER M S, BRADBURY E J.Restoring function after spinal cord injury:towards clinical translation of experimental strategies[J].The Lancet Neurology, 2014, 13 (12) :1241-1256.
[4]	[4]RAMONY, CAJAL, S.Degenerationandregenerationofthe nervous system[M].New York:Oxford University Press, 1928:557.
[5]	[5]SCHIRA J, GASIS M, ESTRADA V, et al.Significant clinical, neuropathological and behavioural recovery from acute spinal cord trauma by transplantation of a well-defined somatic stem cell from human umbilical cord blood[J].Brain, 2012, 135 (2) :431-446.
[6]	[6]CAO Q, WHITTEMORE S R.Cell transplantation:stem cells and precursor cells[J].Handbook of Clinical Neurology, 2011, 109:551-561.
[7]	[7]DAVIESSJA, SHIHCH, NOBLEM, etal.Transplantationof specifichumanastrocytespromotesfunctionalrecovery after spinal cord injury[J].Plo S One, 2011, 6 (3) :e17328.
[8]	[8]GUESTJ, SANTAMARIAAJ, BENAVIDESFD.Clinical translation ofautologous Schwann cell transplantation for the treatment of spinal cord injury[J].Current Opinion in Organ Transplantation, 2013, 18 (6) :682.
[9]	[9]NAKAMURAM, OKANOH.Celltransplantationtherapies for spinal cord injury focusing on induced pluripotent stem cells[J].Cell Research, 2012, 23 (1) :70-80.
[10]	[10]KRABBEC, ZIMMERJ, MEYERM.Neuraltransdifferentiationofmesenchymalstemcellsa critical review[J].Apmis, 2005, 113 (11-12) :831-844.
[11]	[11]PHINNEY D G, ISAKOVA I.Plasticity and therapeutic potential of mesenchymal stem cells in the nervous system[J].Current Pharmaceutical Design, 2005, 11 (10) :1255-1265.
[12]	[12]CHEN Y, TENG F Y H, TANG B L.Coaxing bone marrow stromalmesenchymalstemcellstowardsneuronal differentiation:progress and uncertainties[J].Cellular and Molecular Life Sciences CMLS, 2006, 63 (14) :1649-1657.
[13]	[13]BARZILAY R, MELAMED E, OFFEN D.Introducing transcription factors to multipotent mesenchymal stem cells:making transdifferentiation possible[J].Stem Cells, 2009, 27 (10) :2509-2515.
[14]	[14]KOSHIZUKA S, OKADA S, OKAWA A, et al.Transplanted hematopoietic stem cells from bone marrow differentiate into neural lineage cells and promote functional recovery after spinal cord injury in mice[J].Journal of Neuropathology&Experimental Neurology, 2004, 63 (1) :64-72.
[15]	[15]YANGCC, SHIHYH, KOMH, etal.Transplantationofhuman umbilical mesenchymal stem cells from Wharton's jelly aftercompletetransectionoftheratspinalcord[J].PLo SOne, 2008, 3 (10) :e3336.
[16]	[16]ZHANG L, ZHANG H T, HONG S Q, et al.Cografted Wharton’s jelly cells-derived neurospheres and BDNF promote functional recovery after rat spinal cord transection[J].Neurochemical Research, 2009, 34 (11) :2030-2039.
[17]	[17]HU S L, LUO H S, LI J T, et al.Functional recovery in acute traumatic spinal cord injury after transplantation of human umbilical cord mesenchymal stem cells[J].Critical Care Medicine, 2010, 38 (11) :2181-2189.
[18]	[18]SHANG A J, HONG S Q, XU Q, et al.NT-3-secreting human umbilical cord mesenchymal stromal cell transplantation for the treatment of acute spinal cord injury in rats[J].Brain Research, 2011, 1391:102-113.

Relative Articles(20)

Relative Articles

[1]	Lei ZHOU, Wenwen LYU, Yongzhong DUAN, Wen QIAN, Jishuai CHENG. Research Progress of siRNA Targeting Against HSV-1. Journal of Kunming Medical University, 2024, 45(9): 1-8. doi: 10.12259/j.issn.2095-610X.S20240901
[2]	Jiang WU, He MA, Jinyun JIANG, Na LIU, Ruihuan ZHAO, Yuefeng HE. The Relationship between Arsenic Exposure and DNA Damage of EGFR，PTEN，Kras，and PIK3CA Genes in Arsenic Factory Workers. Journal of Kunming Medical University, 2023, 44(7): 29-33. doi: 10.12259/j.issn.2095-610X.S20230717
[3]	Xie Huan , Tian Jie , Huang Ya Fen . . Journal of Kunming Medical University, 2019, 40(05): 18-23.
[4]	Wang Fu Ke , Zhang Hong , Yang Gui Ran , Xiao Yu , He Chuan , Song En , Li Yan Lin . . Journal of Kunming Medical University, 2019, 40(05): 24-30.
[5]	Li Qin Xue , Li Chuan Jing , Wang Feng . . Journal of Kunming Medical University, 2019, 40(06): 33-38.
[6]	Chen Shan Shan , Yi Min Chun , Zhou Guo Zhong , Pu Yue Chang , Hu Yi , Han Mi Hua , Jin Hua . Effects of CNTF Antibody Block to the Bone Marrow Mesenchymal Stem Cells Transplantated by Abdominal Aortic on the Downstream Signaling Pathways STAT3/Caspase-9 in Spinal Cord Ischemic Reperfusion Injuried Rats. Journal of Kunming Medical University, 2018, 39(08): 5-12.
[7]	Bai Gang , Zhang Hong Tian , Lai Jun , Luo Lin , Zuo Pin , Fan Yao Dong . Joint Therapy by NT-3-HUMSCs and SOCS3 Gene Dilencing in Nerve Regeneration Repair after Spinal Cord Injury in SD Rats. Journal of Kunming Medical University, 2018, 39(03): 31-37.
[8]	Wang Yi Ting , Yang Wei Hong , Zhao Yun Long , Zeng Zhao Shu , Zhang Li Rong . The Enhancer Role of CYP3A4 Intron 10 in CYP3A4 Gene Expression. Journal of Kunming Medical University, 2017, 38(01): 13-17.
[9]	Zhou Qi . Influence of Hypnosis Therapy on Rehabilitation of Patients with Spinal Cord Injury. Journal of Kunming Medical University,
[10]	Dan Ke Ji . . Journal of Kunming Medical University,
[11]	Shao Wen Ping . . Journal of Kunming Medical University,
[12]	Chen Shao Chun . . Journal of Kunming Medical University, 2013, 34(07): 1-1.
[13]	Liu Wei Jun . . Journal of Kunming Medical University,
[14]	Yuan Cong . . Journal of Kunming Medical University,
[15]	. Expression of T-bet and GATA-3 Genes in Rat Allergic Rhinitis Models. Journal of Kunming Medical University,
[16]	. Expression of GDNF,BDNF,NT3 and NT4 in Human Lung Adenocarcinoma Cell Lines A549. Journal of Kunming Medical University,
[17]	Li Kun Lun . Effect of Survivin Gene Expression Inhibition by RNAi on Breast Cancer SKBr-3 Cells. Journal of Kunming Medical University,
[18]	Li Yu . . Journal of Kunming Medical University, 2011, 32(02): 1-1.
[19]	Duan Yan Ping . The Changes of Nogo-A Expression in the Neurons of Acute Complete SCI Rats after GAP-43 Therapy. Journal of Kunming Medical University, 2011, 32(12): 1-1.
[20]	Zhu Xing Bao . . Journal of Kunming Medical University,