Serotyping and PFGE Type of Salmonella Isolates in Yunnan Province

Zhao Jiang; Zou Yan Qiu Shuo; Min Xiang Dong; Cai Tong Jian; Han Xing Meng; Yang Zu Shun; Liu Zhi Tao

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Zhao Jiang , Zou Yan Qiu Shuo , Min Xiang Dong , Cai Tong Jian , Han Xing Meng , Yang Zu Shun , Liu Zhi Tao . Serotyping and PFGE Type of Salmonella Isolates in Yunnan Province[J]. Journal of Kunming Medical University, 2018, 39(02): 30-33.

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Zhao Jiang , Zou Yan Qiu Shuo , Min Xiang Dong , Cai Tong Jian , Han Xing Meng , Yang Zu Shun , Liu Zhi Tao . Serotyping and PFGE Type of Salmonella Isolates in Yunnan Province[J]. Journal of Kunming Medical University, 2018, 39(02): 30-33.

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Serotyping and PFGE Type of Salmonella Isolates in Yunnan Province

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基金：云南省卫生计生委2012年食品安全保障项目 ([2012]659号);

Received Date: 2017-09-21

Abstract

Abstract

Objective To study the serotypes and molecular characteristics of foodborne Salmonella in Yunnan Province using pulsed field gel electrophoresis (PFGE) and to establish PFGE fingerprint database.Methods This study was carried out on the basis of the Serological typing of 322 strains of foodborne Salmonella which was isolated from Yunnan national foodborne disease surveillance from 2013 to 2016. The clustering analysis was conducted on 148 strains of Salmonella DNA restriction enzyme map by using the software of Bio Numerics. Fingerprint database was established through the comparison of clustering analysis correlation on bacterial strain. Results The serotype of 322 strains of Salmonella mainly included A, B, C, D, E, F, G and other 7 groups, among which Salmonella typhimurium was the major type, accounting for 11.4% (37/322) . Cluster analysis was applied using Bio Numerics software in 148 strains of Salmonella DNA restriction enzyme map.According to the different number and the different positions, electrophoresis strips were divided into 102 different PFGE patterns (Figure 1) , which was categorized into 39 clusters if 90% of the strips was similar. Conclusion Foodborne salmonella molecular classification is complex. Salmonella typhimurium is the major type. PFGE belt type presents diversity.
- Salmonella,
- Serotype,
- Salmonella typhimurium,
- Pulsed field gel electrophoresis,
- Molecular typing,
- Foodborne pathogen

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References(21)

References

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