Interactive Effects and Predictive Performace of IL-19 and miR-320 on In-Stent Restenosis After PCI in Patients with Type 2 Diabetes Mellitus Complicated with NSTEMI

Kai YANG; Shangming WANG; Hailiang XIONG; Yonggang CHEN

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Kai YANG, Shangming WANG, Hailiang XIONG, Yonggang CHEN. Interactive Effects and Predictive Performace of IL-19 and miR-320 on In-Stent Restenosis After PCI in Patients with Type 2 Diabetes Mellitus Complicated with NSTEMI[J]. Journal of Kunming Medical University.

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Interactive Effects and Predictive Performace of IL-19 and miR-320 on In-Stent Restenosis After PCI in Patients with Type 2 Diabetes Mellitus Complicated with NSTEMI

Department of Cardiovascular Medicine，Tangshan Central Hospital，Tangshan Hebei 063000，China

Received Date: 2026-01-27

Abstract

Abstract

Objective To explore the interactive effects and predictive performance of interleukin-19 （IL-19） and microRNA-320 （miR-320） for in-stent restenosis （ISR） after percutaneous coronary intervention （PCI） in patients with type 2 diabetes mellitus （T2DM） complicated with non-ST-segment elevation myocardial infarction （NSTEMI）. Methods A total of 210 patients with T2DM complicated by NSTEMI admitted to Tangshan Central Hospital from August 2022 to October 2023 were enrolled and stratified based on ISR status one year post-PCI. Clinical data were compared between the ISR group （n = 34） and non-ISR group （n = 172）. IL-19 and miR-320 levels were compared between groups on postoperative day 1 and 3 months post-operation. The correlations between IL-19 and miR-320, and Killip classification were analyzed. The predictive performance of IL-19, miR-320, and their interactive effects on postoperative ISR was evaluated. Results The Gensini score in ISR group was significantly higher than in non-ISR group （t = 4.524, P < 0.001）. IL-19 and miR-320 levels in the ISR group were higher than those in the non-ISR group （postoperative day 1 IL-19: t = -8.916, miR-320: t = -9.997; 3 months post-operation IL-19: t = -15.498, miR-320: t = -17.175; all P < 0.001）. Logistic regression analysis showed that after adjusting for Gensini score, IL-19 at 3 months post-operation （OR = 1.099, 95%CI: 1.014～1.192） and miR-320 （OR = 1.104, 95%CI: 1.019～1.196） were independent risk factors for ISR（both P < 0.001）. Significant differences in IL-19 and miR-320 levels were observed across different Killip classifications, with both parameters increasing with higher Killip grades （F = 57.143, 153.049, 86.212, 200.270; all P < 0.001）. Spearman correlation analysis revealed positive correlations between IL-19, miR-320, and Killip classification on postoperative day 1 and 3 months post-operation （P < 0.05）. Interaction analysis demonstrated a significant positive multiplicative interaction between IL-19 and miR-320 （interaction term OR = 1.042, 95%CI: 1.011～1.074, P = 0.006）. ROC curve analysis revealed AUC values of 0.742 （95%CI: 0.676～0.800）, 0.768 （95%CI: 0.705～0.824）, and 0.882 （95%CI: 0.830～0.922） for IL-19, miR-320, and their interactive effect, respectively, in predicting ISR after PCI in T2DM patients with NSTEMI, with the interactive effect showing superior predictive performance （P < 0.05）. Bootstrap internal validation showed a corrected AUC of 0.871 for the combined model, with Hosmer-Lemeshow test P = 0.412 > 0.05, indicating good calibration. The combined model provided superior clinical net benefit across a wide range of threshold probabilities. Conclusion IL-19 and miR-320 levels are associated with cardiac function classification in T2DM patients with NSTEMI and serve as independent predictive factors for ISR after PCI. Higher levels of IL-19 and miR-320 indicate increased risk of ISR after PCI in these patients. Combined detection of both biomarkers demonstrates high predictive value for ISR.
- Acute myocardial infarction,
- Type 2 diabetes mellitus,
- Interleukin-19,
- miR-320,
- Percutaneous coronary intervention,
- In-stent restenosis

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