COX5A Relieves Traumatic Brain Injury-Induced Neurological Damage in Mice by Correcting Mitochondrial Homeostasis Imbalance

Guangcai LIU; Hongchen HUANG; Honglan CHEN; Shui JIANG; Yanbin XIYANG; Xu YANG

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Guangcai LIU, Hongchen HUANG, Honglan CHEN, Shui JIANG, Yanbin XIYANG, Xu YANG. COX5A Relieves Traumatic Brain Injury-Induced Neurological Damage in Mice by Correcting Mitochondrial Homeostasis Imbalance[J]. Journal of Kunming Medical University.

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COX5A Relieves Traumatic Brain Injury-Induced Neurological Damage in Mice by Correcting Mitochondrial Homeostasis Imbalance

1.
Department of Laboratory Medicine, The Second Affiliated Hospital of Kunming Medical University, Kunming Yunnan 650101
2.
Institute of Neuroscience, Kunming Medical University, Kunming Yunnan 650500
3.
Department of Laboratory Medicine, The Third People’s Hospital of Yunnan Province, Kunming Yunnan 650011, China

Received Date: 2025-11-16
Available Online: 2026-06-26

Abstract

Abstract

Objective To investigate the neuroprotective effect of cytochrome c oxidase subunit 5A （COX5A） overexpression in mice with traumatic brain injury （TBI） and its association with mitochondrial function. Methods C57BL/6J wild-type （WT） mice aged 4–6 weeks and age-matched COX5A-overexpressing （COX5A-UP） transgenic mice were randomly divided into WT+sham operation group, WT+TBI group, COX5A-UP+sham operation group, and COX5A-UP+TBI group （n = 12 per group, male∶female = 1∶1）. The TBI model was established using the controlled cortical impact method. At 14 days after surgery, neurological and behavioral performance, brain water content, oxygen consumption rate of hippocampal synaptosomal mitochondrial , ATP levels, reactive oxygen species, malondialdehyde, mitochondrial membrane potential and mitochondrial ultrastructure were examined. Results Compared with the WT+sham group, WT+TBI group showed impaired neurological and behavioral performance, increased brain water content, reduced mitochondrial energy metabolism, enhanced oxidative stress and mitochondrial morphological damage （P < 0.05）. Compared with the WT+TBI group, the COX5A-UP+TBI group showed improvements in these neurological, behavioral, edema-related and mitochondrial abnormalities （P < 0.05）. Conclusion COX5A overexpression alleviates TBI-induced neurological impairment, and this effect may be associated with amelioration of synaptosomal mitochondrial dysfunction and preservation of mitochondrial structural homeostasis.
- COX5A,
- Traumatic brain injury,
- Mitochondrial dyshomeostasis,
- Neuroprotection,
- Mouse model,
- Behavior

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References(31)

References

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