The Effect of Microbial Metabolites on Immune Function Reconstruction of Aids Based on Mitochondrial Dysfunction

Mengtian XIA; Beilei LI; Qian CHEN; Xiafei JIN; Feng WEI; Kaixuan WANG

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Mengtian XIA, Beilei LI, Qian CHEN, Xiafei JIN, Feng WEI, Kaixuan WANG. The Effect of Microbial Metabolites on Immune Function Reconstruction of Aids Based on Mitochondrial Dysfunction[J]. Journal of Kunming Medical University.

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The Effect of Microbial Metabolites on Immune Function Reconstruction of Aids Based on Mitochondrial Dysfunction

1.
Department 10 of Infectious Diseases， Shijiazhuang Fifth Hospital， Shijiazhuang Hebei 050000
2.
Department of Pharmacy， Shijiazhuang Municipal Hospital of Traditional Chinese Medicine，Shijiazhuang Hebei 050000
3.
Department 3 of Infectious Diseases， Shijiazhuang Fifth Hospital， Shijiazhuang Hebei 050000
4.
Department of Orthopedics， Xinle Hospital of Traditional Chinese Medicine， Shijiazhuang Hebei 050000，China

Received Date: 2025-06-11

Abstract

Abstract

objective To explore the effect of microbial metabolites on immune function reconstruction of AIDS based on mitochondrial dysfunction. Methods From October 2023 to March 2024, 42 HIV-infected people who received antiretroviral therapy （ART） for 4 years were recruited, including 20 immune non-responders （INR） and 22 responders （IR）. At the same time, 20 non-infected MSM matched by sex and age were selected as the control group, and their HIV, hepatitis B or hepatitis C virus infection were all negative. The immune parameters were analyzed by flow cytometry. The levels of free fatty acid （FFA） and ferrous （Fe²⁺） in plasma were determined by fluorescence assay kit and iron colorimetric assay kit. Results The percentages of CD4⁺ T cells, CD4+CD45RA⁺ T cells and CD4⁺CD45RA⁺CD31⁺ T cells in INR group were lower than those in control group and IR group （P < 0.05）. Compared with IR group, the JC-1 ratio of CD4⁺ T cells, the level of reactive oxygen species （ROS） and the peroxidation/antioxidation of C11-BODIPY in INR group increased （P < 0.05）. The lipid ROS level of CD4⁺ T cells in IR group was higher than that in control group （P < 0.05）. The FFA level and Fe²⁺ level in peripheral blood of patients in INR group were higher than those in control group and IR group （P < 0.05）, and the percentage of CD4⁺CD36⁺ T cells was lower than that in control group and IR group （P < 0.05）, but there was no significant difference between control group and IR group. Conclusion The increase of FFA, a metabolite of flora, is accompanied by the decrease of CD36 receptor expression on CD4⁺ T cells, leading to mitochondrial dysfunction.
- HIV,
- Oxidative stress,
- T lymphocytes,
- Mitochondria,
- Antiretroviral therapy,
- free fatty acids

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References(19)

References

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