Correlation of <i>Helicobacter pylori</i> Antibody Serotypes，Immune Response，and Gut Microbiota in Children with Abdominal-type Henoch-Schönlein <i>Purpura</i> Presenting with Gastrointestinal Hemorrhage

Junying LIU; Wenhui LI; Xin ZHAO; Weina SHI; Xiaojuan WANG

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Junying LIU, Wenhui LI, Xin ZHAO, Weina SHI, Xiaojuan WANG. Correlation of Helicobacter pylori Antibody Serotypes，Immune Response，and Gut Microbiota in Children with Abdominal-type Henoch-Schönlein Purpura Presenting with Gastrointestinal Hemorrhage[J]. Journal of Kunming Medical University.

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Junying LIU, Wenhui LI, Xin ZHAO, Weina SHI, Xiaojuan WANG. Correlation of Helicobacter pylori Antibody Serotypes，Immune Response，and Gut Microbiota in Children with Abdominal-type Henoch-Schönlein Purpura Presenting with Gastrointestinal Hemorrhage[J]. Journal of Kunming Medical University.

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Correlation of Helicobacter pylori Antibody Serotypes，Immune Response，and Gut Microbiota in Children with Abdominal-type Henoch-Schönlein Purpura Presenting with Gastrointestinal Hemorrhage

Department of Infectious Diseases，Hebei Provincial Children’s Hospital，Shijiazhuang Hebei 050000，China

Received Date: 2025-08-16
Available Online: 2026-03-29

Abstract

Abstract

Objective To investigate the correlation between Helicobacter pylori （HP） antibody subtypes, immune response, and intestinal microbiota in children with abdominal Henoch-Schönlein purpura （HSP） complicated by gastrointestinal bleeding. Methods From February 2021 to February 2025, 96 children with abdominal HSP complicated by gastrointestinal bleeding admitted to Hebei Children's Hospital were enrolled as the bleeding group. According to a 1:1 matching principle, 96 children with HSP without gastrointestinal bleeding treated during the same period （non-bleeding group） and 96 healthy children undergoing physical examination （healthy group） were selected as controls. The study compared HP antibody subtypes, immune responses [tumor necrosis factor-α （TNF-α）, interleukin-6 （IL-6）, interleukin-8 （IL-8）, cluster of differentiation 3 positive （CD3⁺）, cluster of differentiation 4 positive （CD4⁺）, cluster of differentiation 8 positive （CD8⁺）, CD4⁺/CD8⁺ ratio], and intestinal microbiota among the three groups. Differences in immune response and intestinal microbiota between the HP antibody-positive and HP antibody-negative groups were compared. Pearson correlation analysis was performed to explore associations among these indicators. Results The incidence of HP antibody type I in the bleeding group was greater than the non-bleeding group, which was greater than the healthy group, while the negative rate of HP antibody showed the opposite pattern （P < 0.05）. In the bleeding group, TNF-α, IL-6, IL-8, CD8⁺, and Escherichia coli levels were higher than in the non-bleeding group, which were higher than in the healthy group. CD3⁺, CD4⁺, CD4⁺/CD8⁺ ratio, Bifidobacterium, and Lactobacillus levels were lower than in the non-bleeding group, which were lower than in the healthy group （P < 0.05）. Among children with abdominal HSP complicated by gastrointestinal bleeding, the HP antibody-positive group showed higher TNF-α, IL-6, IL-8, CD8⁺, and Escherichia coli levels compared to the HP antibody-negative group, while CD3⁺, CD4⁺, CD4⁺/CD8⁺ ratio, Bifidobacterium, and Lactobacillus levels were lower （P < 0.05）. Pearson correlation analysis revealed that TNF-α, IL-6, IL-8, CD3⁺, CD4⁺, and CD4⁺/CD8⁺ were correlated with Escherichia coli, Lactobacillus, and Bifidobacterium （P < 0.05）, and CD8⁺ showed a positive correlation with Escherichia coli （P < 0.05）. Conclusion Children with abdominal HSP complicated by gastrointestinal bleeding have a higher positive rate of HP antibodies and exhibit dysregulated immune response and compromised intestinal barrier function. HP antibody-positive children demonstrate more severe immune dysfunction and intestinal microbiota dysbiosis compared to antibody-negative children, with significant correlations between immune response parameters and intestinal barrier function markers.
- Henoch-Schönlein purpura,
- Abdominal type,
- Gastrointestinal bleeding,
- Pediatric patients,
- HP antibody,
- Immune response,
- Gut microbiota

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References(17)

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