Mechanisms of Resveratrol Inhibiting Glycolysis in Glioblastoma Cells through G6PC

Xuetao YI; Hui LI; Yongsheng HE; Yanjun WANG

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Xuetao YI, Hui LI, Yongsheng HE, Yanjun WANG. Mechanisms of Resveratrol Inhibiting Glycolysis in Glioblastoma Cells through G6PC[J]. Journal of Kunming Medical University.

Citation:

Xuetao YI, Hui LI, Yongsheng HE, Yanjun WANG. Mechanisms of Resveratrol Inhibiting Glycolysis in Glioblastoma Cells through G6PC[J]. Journal of Kunming Medical University.

Xuetao YI, Hui LI, Yongsheng HE, Yanjun WANG. Mechanisms of Resveratrol Inhibiting Glycolysis in Glioblastoma Cells through G6PC[J]. Journal of Kunming Medical University.

Citation:

Xuetao YI, Hui LI, Yongsheng HE, Yanjun WANG. Mechanisms of Resveratrol Inhibiting Glycolysis in Glioblastoma Cells through G6PC[J]. Journal of Kunming Medical University.

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Mechanisms of Resveratrol Inhibiting Glycolysis in Glioblastoma Cells through G6PC

1.
Department of Review and Inspection，Qingdao Pharmaceutical Innovation Service Center，Qingdao Shandong 266000
2.
Research and Development Center
3.
Project Management Department，Yunnan Yunke Biotechnology Research Institute，Kunming Yunnan 650224
4.
Clinical Skills Center，Yunnan Provincial Mental Health Hospital，Kunming Yunnan 650224，China

Received Date: 2025-12-05
Available Online: 2026-03-29

Abstract

Abstract

Objective To investigate whether resveratrol （RES） inhibits glycolysis in glioblastoma （GBM） cells by regulating G6PC and to elucidate the underlying molecular mechanisms. Methods GBM cell lines A172, U87 MG, and U118 MG were treated with RES and transfected with sh-NC, sh-G6PC, pcDNA-NC, and pcDNA-G6PC, respectively. The effects of RES and G6PC on glycolysis, cell viability, and apoptosis in these cells were assessed using CCK-8 assay, specific assay kits, and Annexin V-FITC/PI apoptosis detection kit. Western blot, assay kits, and RT-qPCR were employed to examine the regulatory role of RES on G6PC expression. Results RES treatment （20 μM, 50 μM, and 100 μM） significantly inhibited glycolysis （P < 0.05）, reduced cell viability （P < 0.05）, and induced apoptosis （P < 0.05） in A172, U87 MG, and U118 MG glioblastoma cells. G6PC expression levels in these cells were higher than in human astrocytes （P < 0.001）, and RES downregulated G6PC protein expression （P < 0.01）. G6PC knockdown mimicked the anti-tumor effects of RES （inhibiting glycolysis, reducing viability, and promoting apoptosis, P < 0.05）, while G6PC overexpression showed the opposite effects. Additionally, RES enhanced the inhibitory effects of G6PC knockdown and reversed the glycolysis-promoting effect induced by G6PC overexpression. Conclusion RES inhibits glycolysis in GBM cells by downregulating G6PC expression, thereby suppressing cell proliferation and promoting apoptosis.
- Glioblastoma,
- Resveratrol,
- Glycolysis,
- G6PC,
- Cell viability

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References(25)

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