miR-15a and miR-16-1 Combined with MEG3 for Assessing Recurrence Risk in Endometrial Cancer Patients

Zhaoqiang LI; Yingnan HUANG; Junying DU

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Zhaoqiang LI, Yingnan HUANG, Junying DU. miR-15a and miR-16-1 Combined with MEG3 for Assessing Recurrence Risk in Endometrial Cancer Patients[J]. Journal of Kunming Medical University.

Citation:

Zhaoqiang LI, Yingnan HUANG, Junying DU. miR-15a and miR-16-1 Combined with MEG3 for Assessing Recurrence Risk in Endometrial Cancer Patients[J]. Journal of Kunming Medical University.

Zhaoqiang LI, Yingnan HUANG, Junying DU. miR-15a and miR-16-1 Combined with MEG3 for Assessing Recurrence Risk in Endometrial Cancer Patients[J]. Journal of Kunming Medical University.

Citation:

Zhaoqiang LI, Yingnan HUANG, Junying DU. miR-15a and miR-16-1 Combined with MEG3 for Assessing Recurrence Risk in Endometrial Cancer Patients[J]. Journal of Kunming Medical University.

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miR-15a and miR-16-1 Combined with MEG3 for Assessing Recurrence Risk in Endometrial Cancer Patients

1.
Department of Pathology，Tongchuan People's Hospital，Tongchuan Shaanxi 727100
2.
Department of Pathology，Shaanxi Provincial Cancer Hospital，Xi’an Shaanxi 710061，China

Received Date: 2025-09-12
Available Online: 2026-03-29

Abstract

Abstract

Objective To explore the value of serum microRNA （miR）-15a, miR-16-1 combined with maternally expressed gene 3 （MEG3） in assessing recurrence risk in endometrial cancer patients. Methods A total of 170 endometrial cancer patients who visited Tongchuan People's Hospital from March 2019 to July 2024 were enrolled as the cancer group. They were divided into recurrence group （n = 41） and non-recurrence group （n = 129） based on whether recurrence occurred within one year after treatment. Another 170 healthy individuals who underwent physical examinations at the hospital during the same period were selected as the control group. The expression levels of miR-15a, miR-16-1, and MEG3 in serum were detected by RT-qPCR. RT-qPCR was used to detect serum expression levels of miR-15a, miR-16-1, and MEG3. Serum levels of miR-15a, miR-16-1, and MEG3 were compared between the control group and cancer group, between non-recurrence and recurrence groups, and among different clinicopathological characteristics. Lasso regression combined with COX regression analysis was used to analyze risk factors for recurrence in endometrial cancer patients. ROC and DCA curves were used to analyze the predictive and clinical application value of serum miR-15a, miR-16-1, and MEG3. Dual-luciferase reporter gene assays verified the targeting relationship between MEG3 and miR-15a/16-1. Results Serum miR-15a and miR-16-1 levels in the cancer group were lower than in the control group, while MEG3 levels were higher （P < 0.05）. Serum miR-15a, miR-16-1, and MEG3 levels were correlated with FIGO stage, histological gradie, depth of myometrial invasion, and lymph node metastasis. In patients with FIGO stage III, poorly differentiated tumors, deep myometrial invasion, and lymph node metastasis, serum miR-15a and miR-16-1 levels were lower than in patients with FIGO stage I-II, moderately-to-well differentiated tumors, superficial myometrial invasion, and no lymph node metastasis （P < 0.05）, while MEG3 levels were higher （P < 0.05）. The proportion of FIGO stage III, poor differentiation, and lymph node metastasis, as well as MEG3 levels were higher in the recurrence group compared to the non-recurrence group, while miR-15a and miR-16-1 levels were lower （P < 0.05）. FIGO stage III, poorly differentiated tumors, and low miR-15a, low miR-16-1, and high MEG3 levels were risk factors for recurrence in endometrial cancer patients （P < 0.05）. The AUC and clinical net benefit of serum miR-15a, miR-16-1, and MEG3 alone for predicting recurrence were lower than their combination （P < 0.05）. MEG3 exhibited direct targeted binding with miR-15a and miR-16-1; luciferase activity was reduced following co-transfection of WT-MEG3 with miR-15a/16-1 mimics （P < 0.05）. Conclusion Low expression of serum miR-15a and miR-16-1 and high expression of MEG3 in endometrial cancer patients are associated with patient recurrence. MEG3 can regulate miR-15a and miR-16-1 expression by serving as a competing endogenous RNA. Combined detection of serum miR-15a, miR-16-1, and MEG3 enhances the predictive value and efficacy for assessing recurrence risk.
- Endometrial cancer,
- Recurrence,
- MicroRNA-15a,
- MicroRNA-16-1,
- Maternally expressed gene 3

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References(16)

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