Genomic Analysis of Two Novel Coxsackievirus B2 Strains in 2022

Xiang LI; Zhaoyang CHU; Hengli YANG; Changzeng FENG; Li LI; Jiansheng LIU; Shaohui MA

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Xiang LI, Zhaoyang CHU, Hengli YANG, Changzeng FENG, Li LI, Jiansheng LIU, Shaohui MA. Genomic Analysis of Two Novel Coxsackievirus B2 Strains in 2022[J]. Journal of Kunming Medical University.

Citation:

Xiang LI, Zhaoyang CHU, Hengli YANG, Changzeng FENG, Li LI, Jiansheng LIU, Shaohui MA. Genomic Analysis of Two Novel Coxsackievirus B2 Strains in 2022[J]. Journal of Kunming Medical University.

Xiang LI, Zhaoyang CHU, Hengli YANG, Changzeng FENG, Li LI, Jiansheng LIU, Shaohui MA. Genomic Analysis of Two Novel Coxsackievirus B2 Strains in 2022[J]. Journal of Kunming Medical University.

Citation:

Xiang LI, Zhaoyang CHU, Hengli YANG, Changzeng FENG, Li LI, Jiansheng LIU, Shaohui MA. Genomic Analysis of Two Novel Coxsackievirus B2 Strains in 2022[J]. Journal of Kunming Medical University.

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Genomic Analysis of Two Novel Coxsackievirus B2 Strains in 2022

1.
Key Laboratory for Research and Development of Major Infectious Diseases Vaccine in Yunnan，Institute of Medical Biology，Chinese Academy of Medical Sciences and Peking Union Medical College，Kunming Yunnan 650118
2.
Clinical Laboratory，Kunming Maternal and Child Health Hospital Laboratory，Kunming Yunnan 650031，China

Received Date: 2024-11-05
Available Online: 2026-01-22

Abstract

Abstract

Objective To perform whole-genome analysis of recombinant Coxsackievirus B2 （CVB2） strains isolated in Yunnan Province. Methods Primers spanning the complete CVB2 genomic sequence were designed. Viral genomic fragments were amplified using a segmented PCR strategy, followed by sequencing via primer walking and subsequent sequence assembly. Comprehensive genomic analyses were conducted using DNAStar 7.1, MEGA 7.0, Simplot 3.5.1, and RDP4 software. Results Phylogenetic analysis of the VP1 region confirmed both CVB2 isolates belonged to genotype C. Evolutionary assessment of P1-P3 regions revealed clustering of CVB2 strains with diverse EVB strains in P2 and P3 regions. Simplot and Bootscan analyses identified potential multiple recombination events within non-structural coding regions of isolate 135V3/YN/CHN/2022. Furthermore, RDP4 analysis demonstrated that isolate 135V3/YN/CHN/2022 underwent recombination events involving strains E31/USA/13H4/2016 and CVB5/Swab/HB. HS/CHN/2019. Conclusion The two CVB2 strains, isolated in Kunming Yunnan in 2022 belonged to genotype C and exhibited recombination events, highlighting the need for continuous monitoring of their genomic changes.
- Enterovirus,
- Coxsackievirus B2,
- Whole genome,
- Sequence analysis,
- Recombination analysis

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References(25)

References

[1]	Andino R, Kirkegaard K, MacAdam A, et al. The Picornaviridae family: Knowledge gaps, animal models, countermeasures, and prototype pathogens[J]. J Infect Dis, 2023, 228(Supplement 6): S427-S445.
[2]	Ushioda W, Kotani O, Kawachi K, et al. Neuropathology in neonatal mice after experimental coxsackievirus B2 infection using a prototype strain, Ohio-1[J]. J Neuropathol Exp Neurol, 2020, 79(2): 209-225.
[3]	Glenet M, Heng L, Callon D, et al. Structures and functions of viral 5’ non-coding genomic RNA domain-I in group-B enterovirus infections[J]. Viruses, 2020, 12(9): 919. doi: 10.3390/v12090919
[4]	Hrebík D, Füzik T, Gondová M, et al. ICAM-1 induced rearrangements of capsid and genome prime rhinovirus 14 for activation and uncoating[J]. Proc Natl Acad Sci U S A, 2021, 118(19): e2024251118. doi: 10.1073/pnas.2024251118
[5]	Wang S H, Wang K, Zhao K, et al. The structure, function, and mechanisms of action of enterovirus non-structural protein 2C[J]. Front Microbiol, 2020, 11: 615965.
[6]	Brouwer L, Benschop KSM, Nguyen D, et al. Recombination analysis of non-poliovirus members of the Enterovirus C species; restriction of recombination events to members of the same 3DPol cluster[J]. Viruses, 2020, 12(7): 706. doi: 10.3390/v12070706
[7]	Cordeiro e Cunha J, Silva A L, Nogueira R M, et al. Exuberant hand-foot-mouth disease: An immunocompetent adult with atypical findings[J]. Eur J Case Rep Intern Med, 2020, 7(7): 12. doi: 10.12890/2020_001609
[8]	范丽霞, 汪春翔, 赵生仓, 等. 2018年青海省手足口病病原谱的构成及基因特征分析[J]. 中国病原生物学杂志, 2020, 15(11): 1320-1323. doi: 10.13350/j.cjpb.201116
[9]	Ahmed R, Moaddab A, Hussain SW, et al. A rare case of dilated cardiomyopathy, focal segmental glomerulosclerosis, and Bell's palsy in a 29-year-old male after coxsackievirus infection[J]. Cureus, 2022, 14(6): e26285.
[10]	Zhang Q, Yuan J, Zhao W, et al. Coxsackie B virus-induced myocarditis in a patient with a history of lymphoma: A case report and review of literature[J]. Medicine, 2024, 103(10): e37248. doi: 10.1097/MD.0000000000037248
[11]	Sousa I P Jr, de Lourdes Aguiar Oliveira M, Burlandy F M, et al. Molecular characterization and epidemiological aspects of non-polio enteroviruses isolated from acute flaccid paralysis in Brazil: A historical series (2005–2017)[J]. Emerg Microbes Infect, 2020, 9(1): 2536-2546. doi: 10.1080/22221751.2020.1850181
[12]	Sousa I P Jr, dos Santos F B, de Paula V S, et al. Viral and prion infections associated with central nervous system syndromes in Brazil[J]. Viruses, 2021, 13(7): 1370. doi: 10.3390/v13071370
[13]	Jartti M, Flodström-Tullberg M, Hankaniemi M M. Enteroviruses: Epidemic potential, challenges and opportunities with vaccines[J]. J Biomed Sci, 2024, 31: 73.
[14]	Machado R S, Tavares F N, Sousa I P Jr. Global landscape of coxsackieviruses in human health[J]. Virus Res, 2024, 344: 199367. doi: 10.1016/j.virusres.2024.199367
[15]	Cheng C C, Chu P H, Huang H W, et al. Phylodynamic and epistatic analysis of coxsackievirus A24 and its variant[J]. Viruses, 2024, 16(8): 1267. doi: 10.3390/v16081267
[16]	胡亚芳, 朱汝南. 肠道病毒基因重组研究进展[J]. 国际病毒学杂志, 2022, 29(1): 80-84. doi: 10.3760/cma.j.issn.1673-4092.2021.6.017
[17]	刘煜菡, 张名, 许丹菡, 等. RD、KMB-17及Vero细胞对肠道病毒分离培养敏感性的比较[J]. 中国生物制品学杂志, 2024, 37(08): 917-920. doi: 10.13200/j.cnki.cjb.004272
[18]	Kalendar R, Shustov AV, Schulman AH, et al. Palindromic sequence-targeted (PST) PCR, version 2: an advanced method for high-throughput targeted gene characterization and transposon display[J]. Front Plant Sci, 2021, 12: 691940.
[19]	Li F, Wang D, Su F, et al. Epidemiological, etiological, and serological characteristics of hand, foot, and mouth disease in Guizhou Province, Southwest China, from 2008 to 2023[J]. PLoS Negl Trop Dis, 2025, 19(8): e0013394.
[20]	杨建辉, 张明瑜, 张璐, 等. 2023年河南省急性弛缓性麻痹病例中非脊髓灰质炎肠道病毒病原学及流行病学分析[J]. 病毒学报, 2025, 41(03): 634-644. doi: 10.13242/j.cnki.bingduxuebao.250027
[21]	Zhang Q, Yuan J, Zhao W, et al. Coxsackie B virus-induced myocarditis in a patient with a history of lymphoma: a case report and review of literature[J]. Medicine (Baltimore), 2024, 103(10): e37248. doi: 10.1097/md.0000000000037248
[22]	Fratty I S, Kriger O, Weiss L, et al. Molecular analysis of coxsackievirus B2 associated with severe symptoms of the central nervous system[J]. J Med Virol, 2024, 96(11): e70066. doi: 10.1002/jmv.70066
[23]	周冬, 高孟, 高丽美, 等. 重组柯萨奇病毒构建及其感染性研究[J]. 国际流行病学传染病学杂志, 2019, 46(2): 93-98. doi: 10.3760/cma.j.issn.1673-4149.2019.02.001
[24]	Ang PY, Chong CWH, Alonso S. Viral Determinants That Drive Enterovirus-A71 Fitness and Virulence[J]. Emerg Microbes Infect, 2021, 10(1): 713-724. doi: 10.1080/22221751.2021.1906754
[25]	Yan R, Luo Q, Luo X, et al. Repurposed doxepin targeting host AXL kinase to disrupt viral 2C-mediated immune evasion in Coxsackievirus B infection[J]. Antivir Res, 2025, 244: 106307. doi: 10.1016/j.antiviral.2025.106307

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