Correlation Between Expression of Serum LncRNA TUG1 and MiR-29a-3p and the Severity and Prognosis in Patients with Severe Pneumonia

Zhaozhao LIU; Jun ZHANG; Xiaobo FAN

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Zhaozhao LIU, Jun ZHANG, Xiaobo FAN. Correlation Between Expression of Serum LncRNA TUG1 and MiR-29a-3p and the Severity and Prognosis in Patients with Severe Pneumonia[J]. Journal of Kunming Medical University.

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Zhaozhao LIU, Jun ZHANG, Xiaobo FAN. Correlation Between Expression of Serum LncRNA TUG1 and MiR-29a-3p and the Severity and Prognosis in Patients with Severe Pneumonia[J]. Journal of Kunming Medical University.

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Correlation Between Expression of Serum LncRNA TUG1 and MiR-29a-3p and the Severity and Prognosis in Patients with Severe Pneumonia

1.
Department of Respiratory Medicine，The Fourth Affiliated Hospital of Nanjing Medical University，Nanjing Jiangsu 210000
2.
Clinical Laboratory，Southeast University，Nanjing Jiangsu 210009，China

Received Date: 2025-02-12
Available Online: 2025-12-11

Abstract

Abstract

Objective To analyze the correlation between the expression levels of serum long non-coding RNA taurine up-regulated gene 1 （lncRNA TUG1） and microRNA - （miR） -29A-3p and the severity and prognosis of the patients with severe pneumonia （SP） . Methods A total of 160 SP patients who received treatment in the Fourth Affiliated Hospital of Nanjing Medical University from March 2022 to March 2024, were enrolled as the study subjects. Basic information such as age, gender, and underlying diseases were collected. The patients were stratified into low-risk （n = 20）, moderate-risk （n = 58）, and high-risk groups （n = 82） based on the pneumonia severity index （PSI） score. The expression levels of lncRNA TUG1 and miR-29a-3p in serum using quantitative real-time polymerase chain reaction （qRT-PCR） . The patients were divided into unfavorable and favorable outcome groups based on their 28-day post-ICU admission outcomes. Multivariate ordinal and multivariable Logistic regression models were used to analyze the influencing factors of the disease severity and prognosis, adjusted for confounders. The values of serum lncRNA TUG1 and miR-29a-3p in predicting disease condition and prognosis were represented by receiver operating characteristic （ROC） curves, and correlation analysis was performed using the Pearson method; survival analysis was performed by Kaplan-Meier method. Results The high-risk group had lower oxygenation index and lncRNA TUG1 than the moderate-risk and low-risk groups, and higher interleukin （IL）-6, tumor necrosis factor （TNF）-α, C-reactive protein, and miR-29a-3p than the moderate-risk and low-risk groups （P < 0.05）; the moderate-risk group had lower lncRNA TUG1 than the low-risk group, and higher miR-29a-3p than the low-risk group （P < 0.05）. Compared to the favorable group, the unfavorable group had lower oxygenation index and lncRNA TUG1, and higher IL-6, TNF-α, C-reactive protein, procalcitonin, and miR-29a-3p （P < 0.05）. Correlation analysis indicated that lncRNA TUG1 was positively correlated with the oxygenation index, while IL-6, TNF-α, C-reactive protein, and miR-29a-3p were all negatively correlated. miR-29a-3p was negatively correlated with the oxygenation index and positively correlated with IL-6, TNF-α, and C-reactive protein （P < 0.05）. The area under the curve （AUC） values of the combination of lncRNA TUG1 and miR-29a-3p for predicting disease condition and prognosis were 0.945 and 0.935, respectively, which were higher than those of single prediction （P < 0.05）. Logistic analysis showed that IL-6, TNF-α, and C-reactive protein were all influencing factors for the condition and prognosis of patients with SP. After multiple adjustments, lncRNA TUG1 and miR-29a-3p were both influencing factors for the condition and prognosis of patients with SP （P < 0.05）. Kaplan-Meier analysis showed that the patients with high lncRNA TUG1 expression had significantly higher 28-day survival rate （68/93, 73.12%） compared to those with low expression （31/67, 46.27%）, while the patients with high miR-29a-3p expression had lower 28-day survival rate （21/62, 33.87%） compared to those with low miR-29a-3p expression （78/98, 79.59%）. Conclusion Low expression of serum lncRNA TUG1 combined with high expression of miR-29a-3p in patients with SP may be reliable predictors for high-risk SP and poor prognosis.
- Severe pneumonia,
- Long non-coding RNA taurine up-regulated gene 1,
- MicroRNA-29a-3p,
- Disease condition

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