Expression of LMO2 and CAPRIN1 in Pancreatic Cancer Tissues and Their Correlation with Prognosis

Yuhua XIAO; Lingxin YAN; Jidong CAO; Jinhua CHAI

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Yuhua XIAO, Lingxin YAN, Jidong CAO, Jinhua CHAI. Expression of LMO2 and CAPRIN1 in Pancreatic Cancer Tissues and Their Correlation with Prognosis[J]. Journal of Kunming Medical University.

Citation:

Yuhua XIAO, Lingxin YAN, Jidong CAO, Jinhua CHAI. Expression of LMO2 and CAPRIN1 in Pancreatic Cancer Tissues and Their Correlation with Prognosis[J]. Journal of Kunming Medical University.

Yuhua XIAO, Lingxin YAN, Jidong CAO, Jinhua CHAI. Expression of LMO2 and CAPRIN1 in Pancreatic Cancer Tissues and Their Correlation with Prognosis[J]. Journal of Kunming Medical University.

Citation:

Yuhua XIAO, Lingxin YAN, Jidong CAO, Jinhua CHAI. Expression of LMO2 and CAPRIN1 in Pancreatic Cancer Tissues and Their Correlation with Prognosis[J]. Journal of Kunming Medical University.

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Expression of LMO2 and CAPRIN1 in Pancreatic Cancer Tissues and Their Correlation with Prognosis

1.
Department of Surgery and Anesthesiology，Xingtai Central Hospital，Xingtai Hebei 054000
2.
Gastroenterology Department of Handan Central Hospital，Handan Hebei 056000
3.
Department of Hepatobiliary Surgery，Xingtai First Hospital，Xingtai Hebei 056000
4.
Department of General Surgery，Handan Central Hospital，Handan Hebei 056000，China

Received Date: 2025-07-03

Abstract

Abstract

Objective To investigate the expression of LIM-only protein 2 （LMO2） and cell cycle-associated protein 1 （CAPRIN1） in pancreatic cancer tissues, and their correlation with clinicopathological features and prognosis, and to validate their effects on pancreatic cancer cell function through in vitro knockdown experiments. Methods Ninety pancreatic cancer patients admitted to Xingtai Central Hospital between August 2015 and March 2018 and pathologically confirmed were enrolled. Cancer tissue specimens were collected as the case group, while adjacent non-tumor tissue （>2 cm from the cancer margin, pathologically confirmed without cancer infiltration or abnormal lesions） served as the control group. Immunohistochemistry （IHC） was used to detect the expression levels of LMO2 and CAPRIN1, and their relationships with clinicopathological features including differentiation degree, lymph node metastasis, and TNM staging were analyzed in conjunction with clinical data. A two-year follow-up was conducted, and survival was analyzed using the Kaplan-Meier method, while independent prognostic factors were assessed with the Cox proportional hazards regression model.In vitro experiments, PANC-1 and BxPC-3 cell lines were selected, and specific siRNA targeting LMO2, CAPRIN1, or combined knockdown was performed using Lipofectamine 3000. Knockdown efficiency was verified by qPCR and Western blot, and cell proliferation and migration were assessed using CCK-8, EdU, wound healing, and Transwell assays. Results IHC results showed that the positive expression rates of LMO2 and CAPRIN1 were significantly higher in pancreatic cancer tissues than in adjacent tissues （P = 0.009; P = 0.012）. High LMO2 expression was associated with differentiation degree （P = 0.026） and lymph node metastasis （P = 0.007）, while high CAPRIN1 expression was significantly correlated with TNM staging （P = 0.019）, differentiation degree （P = 0.018）, and lymph node metastasis （P = 0.004）. Correlation analysis revealed a positive correlation between LMO2 and CAPRIN1 expression （P = 0.013）. Follow-up results showed that survival rates in patients with high LMO2 and high CAPRIN1 expression were significantly lower than in those with low expression （P = 0.015; P = 0.021）. Multivariate Cox regression analysis indicated that high LMO2 expression （P = 0.031）, high CAPRIN1 expression（P = 0.019）, TNM stage III+IV （P = 0.005）, poor/moderate differentiation （P = 0.047）, and lymph node metastasis （P = 0.027） were all independent risk factors for prognosis in pancreatic cancer patients. In vitro experimental results showed that knockdown of LMO2 or CAPRIN1 significantly inhibited PANC-1 and BxPC-3 cell proliferation and migration ability, with combined knockdown showing more pronounced effects （P < 0.001）. Conclusion High expression of LMO2 and CAPRIN1 in pancreatic cancer tissue is associated with adverse clinicopathological features and poor survival outcomes. In vitro functional experiments further verified their critical roles in pancreatic cancer cell proliferation and migration, suggesting that LMO2 and CAPRIN1 may serve as potential prognostic biomarkers and therapeutic targets for pancreatic cancer.
- Pancreatic cancer,
- LIM-only protein 2,
- Cell cycle-associated protein 1,
- Immunohistochemistry,
- Clinicopathological characteristics,
- Cox proportional hazards regression,
- Prognosis

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