Expression and Clinical Significance of the Endoplasmic Reticulum PERK/eIF-2α Signaling Pathway in Cervical Cancer

Peipei HE; Ke CHEN; Jun XU; Zhixia WANG; Xuemei YAN

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Peipei HE, Ke CHEN, Jun XU, Zhixia WANG, Xuemei YAN. Expression and Clinical Significance of the Endoplasmic Reticulum PERK/eIF-2α Signaling Pathway in Cervical Cancer[J]. Journal of Kunming Medical University.

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Peipei HE, Ke CHEN, Jun XU, Zhixia WANG, Xuemei YAN. Expression and Clinical Significance of the Endoplasmic Reticulum PERK/eIF-2α Signaling Pathway in Cervical Cancer[J]. Journal of Kunming Medical University.

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Expression and Clinical Significance of the Endoplasmic Reticulum PERK/eIF-2α Signaling Pathway in Cervical Cancer

Department of Gynecology，Nanjing Mingji Hospital /Benji Hospital Affiliated toNanjing Medical University，Nanjing Jiangsu 210000，China

Received Date: 2025-07-14
Available Online: 2026-03-08

Abstract

Abstract

Objective To investigate the expression of the endoplasmic reticulum stress protein kinase R-like endoplasmic reticulum kinase （PERK）/eukaryotic initiation factor 2α （eIF-2α） signaling pathway in cervical intraepithelial neoplasia （CIN） and cervical carcinoma （CC）, and its impact on tumor progression. Methods A total of 58 cervical biopsy specimens from patients diagnosed with cervical cancer (CC) treated at Nanjing Mingji Hospital from January 2022 to April 2023 were collected as the cervical cancer group. Concurrently, 52 cases of cervical intraepithelial neoplasia (CIN II and CIN III) were selected as the control group, and 34 cases of healthy uterine tissues from the same period were chosen as the normal group. Western blot was used to detect the expression of endoplasmic reticulum-mediated related proteins; immunohistochemistry was employed to assess the staining status of PERK/eIF-2α in CC tissues. HeLa cells were divided into the following groups: control group, pcDNA3.1-NC group, overexpressed PERK group, sh-NC group, and PERK interference group. Cell proliferation activity was detected using the CCK-8 assay, and the expression of epithelial cadherin （E-cadherin） was observed by immunofluorescence staining. Apoptosis and cell cycle distribution were analyzed by flow cytometry, and cell migration and invasion ability were evaluated by Transwell assay. Results With increasing CIN grade, the expression of PERK/eIF-2α increased （P < 0.05）, and its expression in CC tissues was significantly higher than that in CIN tissues （P < 0.05）. Compared with the pcDNA3.1-NC group, cells in the PERK overexpression group enhanced proliferation activity, markedly reduced intracellular E-cadherin fluorescence expression （P < 0.05）, and downregulated E-cadherin protein expression （P < 0.05）, accompanied by promotion of cell proliferation, migration, and invasion, as well as inhibition of apoptosis （P < 0.05）. In contrast, compared with the sh-NC group, cells in the PERK interference group showed decreased proliferation activity, significantly increased intracellular E-cadherin fluorescence expression （P < 0.05）, upregulated E-cadherin protein expression （P < 0.05）, along with inhibition of cell proliferation, migration, and invasion, and promotion of apoptosis （P < 0.05）. Conclusion The expression of the endoplasmic reticulum stress-related PERK/eIF-2α pathway is upregulated in uterine intraepithelial neoplasia and CC tissues. Inhibition of its expression can reduce the tumor cell proliferation in CC, thereby slowing tumor progression.
- Cervical cancer,
- Endoplasmic reticulum stress,
- Protein kinase R-like endoplasmic reticulum kinase,
- Eukaryotic initiation factor 2α

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