Mechanism by Which T Cell Immune Structural Proteins Promote the Role of the Hippo Pathway in the Pathogenesis of Preeclampsia

Weiyi ZHANG; Liping YANG; Jiancai MA

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Weiyi ZHANG, Liping YANG, Jiancai MA. Mechanism by Which T Cell Immune Structural Proteins Promote the Role of the Hippo Pathway in the Pathogenesis of Preeclampsia[J]. Journal of Kunming Medical University.

Citation:

Weiyi ZHANG, Liping YANG, Jiancai MA. Mechanism by Which T Cell Immune Structural Proteins Promote the Role of the Hippo Pathway in the Pathogenesis of Preeclampsia[J]. Journal of Kunming Medical University.

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Mechanism by Which T Cell Immune Structural Proteins Promote the Role of the Hippo Pathway in the Pathogenesis of Preeclampsia

Department of Obstetrics，Handan Central Hospital，Handan Hebei 056001，China

Received Date: 2025-03-28

Abstract

Abstract

Objective To explore the mechanism by which T cell immunoglobulin and mucin domain-3 （TIM-3） promotes the Hippo signaling pathway in the pathogenesis of preeclampsia. Methods HTR-8/Svneo cells were divided into a control group （Con）, a recombinant human Tim-3 protein （Tim-3 Fc） group, a YAP1 inhibitor CA3 group and a Tim-3 Fc+CA3 group. Cell proliferation was analyzed by 5- ethynyl-2 ′-deoxyuridine （EdU）. Cell invasion and migration were evaluated by Transwell assay, and cell apoptosis was analyzed by terminal deoxynucleotidyl transferase dUTP nick end labeling （TUNEL） assay. Protein expression levels of TIM-3 and Hippo pathway components in HTR-8/SVneo cells were detected by Western blot. Results Compared to the Con group, protein expression levels of TIM-3, YAP1 and TAZ were up-regulated in HTR-8/SVneo trophoblast cells of the TIM-3 Fc group（P < 0.05）. Compared to the TIM-3 Fc group, protein expression levels of YAP1 and TAZ were down-regulated in trophoblast cells of the TIM-3 Fc+CA3 group （P < 0.05）. Compared to the Con group, the rate of EdU positive cells in HTR-8/SVneo trophoblast cells in TIM-3 Fc group was increased significantly （P < 0.05）. Compared to the TIM-3 Fc group, the rate of EdU positive cells and the number of apoptotic cells in HTR-8/SVneo trophoblast cells of the TIM-3 Fc+CA3 group were decreased significantly （P < 0.05）. Compared to the Con group, the numbers of HTR-8/SVneo trophoblast cells in TIM-3 Fc group were increased significantly （P < 0.05）. Compared to the TIM-3 Fc group, the number of HTR-8/SVneo trophoblast cells in TIM-3 Fc+CA3 group were decreased significantly （P < 0.05）. Conclusions Tim-3 activates the Hippo pathway by interacting with YAP1 in trophoblast cells, thus promoting cell proliferation, invasion and migration.
- T cell immunoglobulin and mucin domain-3,
- Preeclampsia,
- Hippo pathway,
- Trophoblast cell

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References(21)

References

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